From jemfly at optusnet.com.au Sat Sep 16 21:16:18 2006 From: jemfly at optusnet.com.au (Gwendolyn) Date: Sat Sep 23 06:24:35 2006 Subject: [Genstructure] GUS/GFP tagged promoters or GUS/GFP fusion proteins Message-ID: <1158459377.929755.7320@e3g2000cwe.googlegroups.com> I am a Genetics students and I've got the following doubt: I read some papers that described experiments where plants were transformed with both a GUS and a GFP tagged promoter (same promoter in both cases) or with a GUS and a GFP fusion protein (same protein in both cases). Why is it that two different reporter constructs were used for transformation? Perhaps because there is always the possibility that GFP will not fluoresce (due to tagging/fusion), or that the glucuronidase gene will not be (properly) transcribed, due to the same reasons? Cheers Gwen From d.carley at wtconference.org.uk Wed Sep 27 06:32:02 2006 From: d.carley at wtconference.org.uk (Debbie Carley) Date: Sat Sep 30 16:09:02 2006 Subject: [Genstructure] Open Lecture on Cellular Differentiation: Enabling Stem Cell Research. Wed 18th Oct 2006, Hinxton, Cambridge, UK Message-ID: <098B617579F1AC4696C59B41A8A01AAA0104073D@hhlmail.hinxton.wellcome.ac.uk> OPEN LECTURE SERIES Revolutionizing Life Sciences: Tools, Innovations & Insights USA West Coast | USA East Coast | United Kingdom | Singapore | Japan In celebration of the 25th anniversary of Applied Biosystems, The Applera Charitable Foundation is proud to sponsor an inspiring series of lectures entitled "Revolutionizing Life Sciences: Tools, Innovations & Insights." In this series, leading scientists and researchers will gather to discuss the past, present and future of important developments in the life sciences and the role technology has played in spurring these breakthroughs. Please join us at Hinxton, Cambridge, UK for our next event: Cellular Differentiation: Enabling Stem Cell Research DATE: Wednesday, October 18, 2006 TIME: 13:00 - 17:00 LOCATION: The Francis Crick Auditorium, Wellcome Trust Conference Centre, Hinxton, Cambridge, UK ( http://www.wtconference.org.uk ) MODERATORS: Michael Ashburner, Ph.D., Sc.D., F.R.S. University of Cambridge Elizabeth Robertson, Ph.D., F.R.S. Wellcome Trust Centre for Human Genetics SPEAKERS: Austin Smith, Ph.D., F.R.S. Chairperson, Institute for Stem Cell Biology University of Cambridge "The Ground State of Pluripotency" Roger Pedersen, Ph.D. Professor of Regenerative Medicine Department of Surgery and Cambridge Institute for Medical Research University of Cambridge "Mechanisms of Pluripotency and Epigenesis in Human Embryonic Stem Cells" Azim Surani, Ph.D., F.R.S. Mary Marshall and Arthur Walton Professor of Physiology and Reproduction University of Cambridge "Germ Line and Pluripotent Stem Cells" ************************************************************************ ******* Reception to follow. This event is FREE and open to the public, but seating is limited. Please register in advance at http://info.appliedbiosystems.com/sls and arrive early to secure your seat. The first 200 people to arrive will receive a special gift! ************************************************************************ ******* Dr Deborah Carley Science Programme Manager / Head of Conference Centre Wellcome Trust Genome Campus Hinxton Cambridge CB10 1RQ tel: +44 (0)1223 495006 fax: +44 (0)1223 495141 email: d.carley@wtconference.org.uk Check out the Wellcome Trust Conference Programme at www.wellcome.ac.uk/conferences and the Wellcome Trust Conference Centre at www.wtconference.org.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/genstruc/attachments/20060927/9178f968/attachment.html