From sbe at biochem.usyd.edu.au Sun Jun 1 22:02:49 1997 From: sbe at biochem.usyd.edu.au (Simon Easterbrook-Smith) Date: Mon Mar 7 07:29:59 2005 Subject: Affinity Constant References: <5CAC1671BB5@rna.bio.mq.edu.au> Message-ID: Hi Chris (1) Make Fab fragments from your Ab (I assume MAb, if not then you will not get a unique binding constant) and label the Fab with 125I. (2) Titrate labelled Fab into suspension of your ag-positive cells. (3) Measure free and bound Fab (from 125I radioactivity of cells and S/N) under equilibrium conditions (ie when [bound] is constant with time). (4) Plot [bound] vs [free], fit appropriate eqn to data (usually non-cooperative binding). (5) Best to correct for non-specific binding using labelled irrelevant (but isotype-matched) Fab. (6) Good luck. In article <5CAC1671BB5@rna.bio.mq.edu.au>, cweir@RNA.BIO.MQ.EDU.AU ("Chris Weir") wrote: || Hi all, || Could someone please be of assistance! || How do you determine the affinity constant of an antibody, when we || are unable to purify the antigen (as it is expressed on the surface || of the cell1)??? || || Thanks in advance || || Chris Weir || Macquarie University Simon -- _______________________________________________________________ Dr Simon B Easterbrook-Smith Voice: (+) 612 9351 3905 Department of Biochemistry FAX: (+) 612 9351 4726 University of Sydney Email: sbe@biochem.usyd.edu.au Sydney NSW 2006 AUSTRALIA _______________________________________________________________ Under U.S. Code, Title 47, Chapter 5, Subchapter II, ?227, all unsolicited commercial E-mail sent to my address from a U.S. account is subject to a fee of $500.00 U.S. By E-mailing me you accept these terms. cf. _______________________________________________________________ From EB15 at le.ac.uk Mon Jun 2 08:07:54 1997 From: EB15 at le.ac.uk (Dr E. Buxbaum) Date: Mon Mar 7 07:29:59 2005 Subject: Homemade Chemiluminescent Substrates? References: <338EFCA7.41C6@risotto.mit.edu> <338ECA5B.6690@uic.edu> Message-ID: <5mugja$1ds@falcon.le.ac.uk> Keld Sorensen wrote: >Say you use 10 ml of a 1:500 dilution of a primary antibody that cost >you $1,000/mg, then the antibody cost is $20 per blot. If you had to use >say a 1:250 dilution (weak affinity antibody) then the cost would be $40 >per blot!! This antibody solution would be reused many, many times. The costs per blot are, therefore, much lower. From ghermans at luc.ac.be Mon Jun 2 02:37:55 1997 From: ghermans at luc.ac.be (Guy Hermans) Date: Mon Mar 7 07:29:59 2005 Subject: Route of immunisation???? References: <338E908D.C9E@alpha2.curtin.edu.au> Message-ID: In article <338E908D.C9E@alpha2.curtin.edu.au>, Grace Ho wrote: > Hello everyone, > I am pretty confuse here about the route of immunisation!!! I am > currently working with a mouse model where I take my protein antigen in > Freund's adjuvant.... and immunised the victim via intraperitoneal route > .... in the hope of stimulating a good systemic response as well as good > T-cells reponse.... > However, I have been receiving advice from people (immunologists) > and found that ip. immunisation is not a good way to go about if I want > a good systemic/T-cells reponse..... They all recommended subcutaneous > injection or intradermal injection.... > Problem is I am so used to ip. route immunisation and my work have > been sort of based on ip. immunisation.... and things have not been > working very well for me... and on the other hand, if I change the route > of immunisation.. then my work is sort of down the drain!!!! > Does anyone have any insight to this!!!!????? Please!!!?? > Hello again Grace, there is a little insight available on this subject, yes. The route of immunisation is equally important in obtaining a 'good' immune respone as the choise of antigen & adjuvants. Apparently, the topological route of entry selects for a specific subset of antigen presenting cell. In other words; ip/freunds will preferentially use the intraperitoneal cavity macrophages as APC's, while the intrademal route will locate the antigen near the Langerhals cells there. These will take up the antigen and migrate to the draining lymph nodes, maturing to dendritic cells there. Both APC-derived cytokines and adhesion molecules on the APC seem to make a difference in the resulting response. The type of antigen presenting cell influences the response you'll get; therefore, route of entry will -indirectly- influence the response as well. Sorry to say , but your results obtained by ip immunisation will be hard to match with the ones obtained through id. However, you might make a point in your project by comparing the results of both methods. Best of luck, Guy -- Guy Hermans, PhD student Ms research Unit Immunology research group Dr. L. Willems-Institute Dept. of Physiology, LUC University Campus University Campus B-3590 Diepenbeek B-3590 Diepenbeek Belgium Belgium Voice ++32(0)11/26.92.07 Fax ++32(0)11/26.92.09 From mrc7 at cam.ac.uk Mon Jun 2 10:51:48 1997 From: mrc7 at cam.ac.uk (Mike Clark) Date: Mon Mar 7 07:29:59 2005 Subject: Affinity Constant References: <5CAC1671BB5@rna.bio.mq.edu.au> Message-ID: In article <5CAC1671BB5@rna.bio.mq.edu.au>, Chris Weir wrote: > > Hi all, > Could someone please be of assistance! > How do you determine the affinity constant of an antibody, when we > are unable to purify the antigen (as it is expressed on the surface > of the cell1)??? > > Thanks in advance > > Chris Weir > Macquarie University > Hi Chris, It really depends on how accurate you need to know the affinity constant? For most antibodies I usually refer to the avidity of binding ie the affinity constant for a whole antibody. The easiest way by far to get a very reasonable estimate of this constant which works for almost any detection system is to determine in a titration the concentration of antibody which gives 50% of maximal binding to antigen. If you then assume that the total input antibody is very nearly the same as free antibody (which is usually the case for antibody affinities) the kd is equal to this concentration. If you wish to express it as ka then just calculate the recipricol value. To get the single site affinity you could repeat the experiment with Fab fragments. Using computer curve fitting it is very easy to determine the 50% value with a high degree of accuracy and thus I rarely resort to transformations such as Scatchard Analysis. In fact the biggest error in this process is the determination of antibody concentration. Mike Clark, mrc7@cam.ac.uk http://www.path.cam.ac.uk/~mrc7/ -- o/ \\ // || ,_ o Dr. M.R. Clark, Division of Immunology <\__,\\ // __o || / /\, Cambridge University, Dept. Pathology "> || _`\<,_ // \\ \> | Tennis Court Rd., Cambridge CB2 1QP ` || (_)/ (_) // \\ \_ Tel.+44 1223 333705 Fax.+44 1223 333875 From cweir at RNA.BIO.MQ.EDU.AU Sun Jun 1 21:16:28 1997 From: cweir at RNA.BIO.MQ.EDU.AU (Chris Weir) Date: Mon Mar 7 07:29:59 2005 Subject: Affinity Constant Message-ID: <5CAC1671BB5@rna.bio.mq.edu.au> Hi all, Could someone please be of assistance! How do you determine the affinity constant of an antibody, when we are unable to purify the antigen (as it is expressed on the surface of the cell1)??? Thanks in advance Chris Weir Macquarie University From zjtangb at PUB.ZHANJIANG.GD.CN Mon Jun 2 08:48:43 1997 From: zjtangb at PUB.ZHANJIANG.GD.CN (zjtangb) Date: Mon Mar 7 07:29:59 2005 Subject: job-want Message-ID: <3393A77D.40CB@pub.zhanjiang.gd.cn> Dear Professor: My name is Tang Bin. I'm an associate professor of Immunology at Guangdong Medical College in Zhanjiang. I am writing to you to explore the possibility of spending one to two years working in your laboratory as a visiting scholar. Enclosed please find a complete CV of my education, training, researching , teaching experience, publications and references . As you can see from my CV, I have had experience in clinical immunology and immunopharmacology. I am now doing research in the role of cytokines(IL-4.6.8) & their receptors in chronic liver dieases, the molecular mechanism of several cytokines and adhesive molecules inducing glomerular mesangial cell apoptosis, the pathway of IFN-( inducing blood vessel endothelium cell growth and proliferation, etc. And also, doing research in immunological regulation of two kinds of BRMs(extracted from herbage). In the wake of these research, I am more interested in the following problems: 1) The clinical cytokines net. 2) The mechanism of cytokine and adhesive molecule as well as their mAbs in transducing singnals to lymphocytes or other cells. 3) The possibility of treating some diseases via regulating cytokine or adhesive molecule gene transcription and expression by biological response modifiers. I am eager to do something in these fields under your guidance. I am confident that I can do it better if you offer me this opportunity. I am confident of my ability to make a contribution, however small, to your ongoing research projects. My College has granted me an extended leave of absence, which enables me to obtain research experience and interaction abroad.I wish I could find words to express the importance of an appointment as a visiting scholar, for I know how I could learn, and how much China could gain from my working under your guidance. Please tell me whether or not you are in a position to offer me this opportunity. If convenient, please comment also on the possibility of my receiving any financial support. I hope all the above will give you a better idea of my background, and bring me a good luck. If you need any other additional information, please do not hesitate to contact me. Thank you for your consideration. I look forward to hearing from you soon. Yours sincerely, Dr Tang Bin Department of Microbiology & Immunology Guangdong Medical College Zhanjiang(524023), Guangdong PRC. E-mail: zjtangb@pub.zhanjiang.gd.cn Enclosure: Curriculum Vitae CURRICULUM VITAE ¢ñ. GENERAL INFORMATION Name: Tang Bin Description: 33 years old/male/married/178 cm/85 Kg Address: Department of Microbiology & Immunology, Guangdong Medical College, Zhanjiang(524023), Guandong, PRC Telephone: 86-0759-2281544-3035(Office) 86-0759-2234784-6003(Home) E-mail: zjtangb@pub.zhanjiang.gd.cn Fax: 86-0759-2284104 Citizen: PRC Date of birth: May 3, 1964 Place of birth: Guichi City, Anhui Province, PRC Health: excellent ¢ò. EDUCATION 1989-1992: Wannan Medical College (Wuhu city, Anhui province, PRC) Department of Microbiology & Immunology, Postgraduate student and then got M.D. Major Courses: Immunology, Immunopharmacology, Pharmacology, Medical Microbiology, Cellular Genetics, Molecular Biology, Nuclear Medicine, Computer Science, Biochemistry, Medical Statistics, English, etc. 1979-1982: Chaohu Medical School, Anhui, PRC. Majoring in program of Medical Labrotary Technique. 1975-1979: Yinhui Middle School, Anhui, PRC ¢ó. TRAINING 1. 17 April, 1995-27 August, 1995 Training program for cytokines research technique, Department of Immunology, Beijing Medical University, Beijing 2. 1 May, 1994-30 july, 1994 Training program for clinical immunology research technique, Clinical Immunology Laboratory of Changhai Hospital, Second Military Medical University, Shanghai ¢ô. RESEARCHING & TEACHING EXPERIENCE 1992-present: Department of Microbiology & Immunology at Guangdong Medical College Current courses: Molecular Immunology (for graduate students), Medical Microbiology & Immunology(for undergraduates), Clinical Immunology & Immunologic test (and also for undergraduates ) Current reasearch: Clinical Immunology and Immunopharmacology 1989-1992: Department of Microbiology & Immunology at Wannan Medical College Research area: Immunopharmacology 1982-1989: Clinical Immunology Laboratory of Anqing Psychiatric Hospital, Anhui, PRC ¢õ. PUBLICATIONS 1. Effect of stress on mice's immune function and hypothalamic-hypophyseal-thyroid axis. Shanghai Journal of Immunology. 1993; 13 (6): 340-341 2. The effect of TRH & Buzhong Yiqi Tang on RBC immunologic function recovery of amputation mice. Pharmacology and Clinics of Chinese Materia Medica. 1993; 9(4): 6-8 3. Effect of Buzhong Yiqi Tang on the recovery of red-cell immune adhesive function in amputated mouse. Journal of Guangdong Medical College. 1993; 11(4): 195-197 4. Advances in the immuno-pharmacological study of Astragalus mongolius. Journal of Guangdong Medical College. 1993; 11(3): 173-176 5. Advances in the study of anti-sperm immuno-sterility. Journal of Wannan Medical College. 1993; 12(3): 238-240 6. The effect of mixture of Astragalus mongolius and pig Ig on immunological function of mice. Journal of Wannan Medical College. 1993; 12(1): 5-7 7. Effect of TRH and Buzhong Yiqi Tang on NK activity and endocrine in stress mice. Chinese Journal of Integrated Traditional and Western Medicine. 1994; 14(2): 104-105 8. Effect of thyrotropin releasing hormone (TRH) on RBC immunologic function of mouse. Chinese Journal of Experimental & Clinical Immunology. 1994; 6(2): 4-6 9. Effect of thyrotropin releasing hormone on immune function in stressed mice. Chinese Journal of Pharmacology and Toxicology. 1994; 8(3): 233-234 10.Changes of red blood cell immune function in several kinds of nervous system infections diseases. Journal of Applied Clinical Pediatrics. 1994; 9(6):323-325 11.Advances in the experimental study of decoction Buzhong Yiqi. Journal of Guangdong Medical College. 1994; 12(4): 324-327 12.Investigation of red blood cell immune function in newborn common diseases. Journal of Proper Technique for Diagnosis and Therapy. 1994; 12(4): 4-5 13.£Ô£Ò£È¤È²¹ÖÐÒæÆøÌÀ¤ÎºÏÓäˤè¤ë¥¹¥È¥ì¥¹¥Þ¥¦¥¹NKϸ°û¤Î»îÐÔ¼°¤ÓÄÚ·ÖÃÚ¤Ë Ó뤨¤ëÓ°Ïì. The Journal of Chinese Traditional and Western Medicine(Japanese). 1995; 6(1): 93-95 14.Advances in the immunopharmacology study of Buzhong Yiqi Tang. Chinese Traditional Patent Medicine. 1995; 17(1): 42-43 15.Clinical significance of serum sIL-2R level in patients with cirrhosis. Chinese Journal of Clinical Hepatology. 1995; 11 (suppl): 16-17 16.Relationship between serum sIL-2R level and primary hepatocellular carcinoma. Shanghai Journal of Immunology. 1995; 15(2): 105-106 17.Erythrocytic immune function of idiopathic thrombocytopenic purpura. Chinese Journal of Hematology. 1995; 16(3): 130-131 18.Serum sIL-2R level in aged patients with primary hepatocellular carcinoma. Chinese Journal of Clinical Hepatology. 1995; 11(suppl):64-65 19.Clinical significance of Serum sIL-2R level variance in patients with heart failure. Chinese Journal of Critical Care Medicine. 1995; 15 (3): 4-6 20.Serum sIL-2R level in patients with digestive tract malignant tumor. Labeled Immunoaassays and Clinical Medicine. 1995; 2(2): 118-119 21.Experimental study on effect of Buzhong Yiqi Tang on red-cell immune function & hypothalamic-hypophyseal-thyroid axis in stress mice. Chinese Journal of Immunology. 1995; 11(Suppl): 300-302 22.Relationship between RBC-SOD, LPO and RBC immune function in patients with heart & brain vessel diseases. Chinese Journal of Immunology. 1995; 11(Suppl): 337-338 23.Study of relationship between sIL-2R and red-cell immune function in aged patients with gastric diseases. Chinese Journal of Gerontology. 1995; 15(special, second): 2-4 24. Clinical significance of serum sIL-2R variety in patients with heart & brain vessel diseases. Chinese Journal of Practical Medicine. 1995; 15(8): 474-475 25.Way to training experimental ability of undergraduates. Journal of Guangdong Medical College. 1995;13(4):369-370 26.Study of relationship between sIL-2R and red-cell immune function in patients with nasopharyngeal carcinoma. Journal of Clinal Immunology. 1995; 3(5):20-21 27.Study of relationship between sIL-2R and red-cell immune function in patients with kidney diseases. Shanghai Journal of Immunology. 1996; 16(1): 51 28.Role of TNF-¦Á in heart & brain vessel diseases developing. Chinese Journal of Gerontology. 1995; 16(1): 4-5 29. Regulatory effect of Lingqi Anshen liquor on erythrocytic immune function and antioxidation in immunosuppressed mice. Chinese Journal of Integrated Traditional and Western Medicine.1996;16(3) :167-169 30.Role of sIL-2R in aged chronic bronchopneumonia. Guangdong Medical Journal. 1996; 17(4):240-241 31.Distinctive diagnostic value of testing sIL-2R in patients with tuberculosis or cancerous thoracal accumulated liquid. Journal of Practical Pulmonology. 1996; 3(1): 30-32 32.Clinical significance of erythrocytic immune adhensive function test in childhood's common diseases. Journal of Guangdong Medical College. 1996; 14(2): 127-128 33.Preliminary investigation of the erythrocytic immune function in children with malnutrition . Journal of Guangdong Medical College. 1996;14(3):236 34.A preliminary study on the relationship between serum interleukin-6 and acute cerebral infarction. Journal of Guangdong Medical College. 1996; 14(3): 216-217 35.Relationship between Serum sIL-2R level and red-cell immune fuction in patients with digestive ulcer. Shanghai Journal of Immunology. 1996; 16(3): inside front cover 36.Serum soluble IL-2 receptor level and RBC immune function in patients with chronic gastritis. Chinese Journal of New Gastroenterology. 1996; 4(9): 509-510 37.Study of RBC immune fuction in patients with bronchopneumonia.Chinese Clinical Medical. 1996; 1: 353-354 38.Pharmacological study on Lingqi Anshen liquor. Chinese Traditional Patent Medicine. 1996; 18(11):33-35 39.Role of IL-8 in chronic renal failure. Chinese Journal of Kidney. 1996; 12(6): 268-270 40.Serum level of IL-8 in systemic lupus erythematosus.Chinese Journal of Dermatology.1997;30(1):31 41.Role of TNF-¦Á, IL-6 and IL-8 in primary hepatic carcinoma and cirrhosis. (has been accepted by Journal of Guangdong Medical College) 42.Study on the serum TNF-(, IL-6 & IL-8 levels before and after operation in patients with digestive tract tumor. (has been accepted by Shanghai Journal of Immunology) 43.Role of IL-6, IL-8 & C-GSF in the children's idiopathic thrombocytopenia purpura. (has been accepted by Chinese Journal of Hematology) ¢ö. REFERENCES Chen Qun, Professor of Microbiology & Immunology, Chairman of the Department of Microbiology & Immunology, Guangdong Medical College, Zhanjiang(524023),Guangdong, PRC Wu Minyu, Professor of Immunology, my tutor, Chairman of the Department of Microbiology & Immunology, Wannan Medical College, Wuhu(241001), Anhui, PRC From amcbride at bilbo.bio.purdue.edu Mon Jun 2 14:01:52 1997 From: amcbride at bilbo.bio.purdue.edu (Ali McBride) Date: Mon Mar 7 07:29:59 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> Message-ID: <339318A0.270E@bilbo.bio.purdue.edu> David Aldridge wrote: > > In article <338EFCA7.41C6@risotto.mit.edu>, Thomas Cameron > wrote: > > > Company kits end up costing $3-5 per western blot which is 90% of the > > cost of the whole procedure. > > > > People must have some good homemade HRP (and AlkPhos) Chemiluminescent > > Detection protocols or references? > > > > Could you send me one or point me to it? Thanks. > > *Luminol 4mg/ml in DMSO 1ml > *p-iodophenol 1mg/ml in DMSO 1ml > 1M Tris.HCL pH 7.5 0.6ml > H2O2 (30%) 5 microL > H2O 7.5ml > > *Store at -20 > Mix this stuff up fresh a few minutes before use. > Works for me! Where can I buy the luminol and the p-iodophenol from Thanks in advance Ali From jrbagley at fas.harvard.edu Mon Jun 2 13:32:08 1997 From: jrbagley at fas.harvard.edu (Jessamyn Bagley) Date: Mon Mar 7 07:29:59 2005 Subject: IL-4 antibody Message-ID: <5mv3j8$eqk$1@news.fas.harvard.edu> I'm looking for a source of non-neutralizing anti-murine/human IL4. I need quite a bit of it, so I'd really like to get a hybridoma and purify the antibody myself. Any suggestions would be greatly appreciated! -- Jessamyn Bagley jrbagley@husc.harvard.edu From ecastro at physci.ucla.edu Mon Jun 2 11:20:19 1997 From: ecastro at physci.ucla.edu (Edmundo Castro) Date: Mon Mar 7 07:29:59 2005 Subject: Homemade Chemiluminescent Substrates? References: <338EFCA7.41C6@risotto.mit.edu> <338ECA5B.6690@uic.edu> Message-ID: <3392F2C3.7E7C@physci.ucla.edu> I have reused primary Ab >15 times with good results. Save $ =) EC From ahfell at netmatters.co.uk Mon Jun 2 11:38:10 1997 From: ahfell at netmatters.co.uk (Andrew Fell) Date: Mon Mar 7 07:29:59 2005 Subject: Route of immunisation???? References: <338E908D.C9E@alpha2.curtin.edu.au> Message-ID: <3392F6F2.5988@netmatters.co.uk> Guy Hermans wrote: > > Apparently, the topological route of entry selects for a specific subset > of antigen presenting cell. In other words; ip/freunds will preferentially > use the intraperitoneal cavity macrophages as APC's, while the intrademal > route will locate the antigen near the Langerhals cells there. These will > take up the antigen and migrate to the draining lymph nodes, maturing to > dendritic cells there. Both APC-derived cytokines and adhesion molecules > on the APC seem to make a difference in the resulting response. > > The type of antigen presenting cell influences the response you'll get; > therefore, route of entry will -indirectly- influence the response as > well. > > Sorry to say , but your results obtained by ip immunisation will be hard > to match with the ones obtained through id. However, you might make a > point in your project by comparing the results of both methods. > Grace, I should do some Medline searching on this. I think there is literature out there on route of immunisation and this is one of those subjects where every Ph.D. student re-invents the wheel. BTW, an excellent way to use Medline is the NCBI Pubmed search engine at http://www.ncbi.nlm.nih.gov/PubMed Personally, I think material injected into the PEC drains into the blood quickly and then gets pretty much everywhere. However, I guess the Freunds might keep it in the PEC as a big oily blob. Andy Fell From liawas at pc.jaring.my Mon Jun 2 17:25:54 1997 From: liawas at pc.jaring.my (Lea Johnsiul) Date: Mon Mar 7 07:29:59 2005 Subject: OUTBREAK OF ACUTE VIRUS INFECTION IN SARAWAK, MALAYSIA. Message-ID: <33934871.4A65@pc.jaring.my> An outbreak of an acute virus infection was detected in Sarawak, Malaysia beginning from 14 April 1997. Up till today (2 June 1997), 15 children have died from this disease. Their ages ranged from 5 months to 3.5 years; nine were males and six were females. All the cases came from different localities, but six were from within the same district. All cases were very ill when admitted to hospital and died within 24 hours of admission. All presented with similar clinical features which were as follows:- Clinical features: - fever of 2-3 days duration - involvement of the nervouse system which manifested as fits and paralysis of one side of the upper limbs - poor systemic perfusion - end stage : cardiogenic shock due to myocarditis. Echocardioram shows poor contractability of left ventricle Other significant findings, Chest x-ray - pulmonary oedema Cerebro-spinal fluid -suggestive of aseptic meningitis the following have been excluded serologicay : Japanese encephalitis Dengue Yellow fever Virus culture: Serum and cerebro-spinal fluid cultured in: 1. baby hamster kidney cells medium MEM with 1% foetal bovine serum 2. pig kidney cells (PS) medium Leibovitz 15 with 1% foetal bovine serum 3. mosquito cells C6/36 medium Leibovitz 15 with 1% foetal bovine serum Cytopathic effect (CPE) noted in medium (3) for cerebrospinal fluid from one patient Throat and rectal swabs will be cultured in vero-primate cell line today (2/5/1997) As we have yet to come to a conclusive diagnosis of this condition, we would bevery grateful if anyone could provide some information or suggestion as to what organism we are dealing with. This is essential for control purposes. Please contact : Sarawak Health Department Email : JKNS2@po.jaring.my Tel: 60-82-256566 Fax: 60-82-424959 From ronlab at UIC.EDU Mon Jun 2 15:25:13 1997 From: ronlab at UIC.EDU (ronlab) Date: Mon Mar 7 07:29:59 2005 Subject: Q: protein A instead of a secondary Ab Message-ID: <33932CC9.3DA@uic.edu> Hi! I would like to know if there are any limitations on using protein A or protein G instead of a secondary Ab for immunostaining and FACS. Does anyone has relevant experience (positive or negative)? Are there any published protocols for these applications? Your suggestions and comments will be greatly appreciated. Eugene Kandel. U09577@uic.edu From tore001 at pn.itnet.it Mon Jun 2 16:09:50 1997 From: tore001 at pn.itnet.it (AT) Date: Mon Mar 7 07:29:59 2005 Subject: Helicobacter sito ITALIANO Message-ID: <5mvcs9$seq@dns2.IT.net> Helicobacter - sito ITALIANO For ITALIAN people: the most complete site on Helicobacter pylori is at: VI SFIDO A TROVARE UN SITO CON MAGGIORI INFORMAZIONI ------------------------------------------- dr Alberto Torelli (1997) tel/sgr/fax 019-506054 Alberto.Torelli@pn.itnet.it ------------------------------------------- From dmike at ix.netcom.com Mon Jun 2 22:15:43 1997 From: dmike at ix.netcom.com (David J. Mike) Date: Mon Mar 7 07:30:00 2005 Subject: Need help! for parisitic illness in Thailand Message-ID: <5n01sf$4q0@dfw-ixnews6.ix.netcom.com> I am looking for help for a young missionary in Thailand as follows: TO MY "URGENT PRAYER WARRIOR" NETWORK: While at the 24 hour prayer desk at the U.S. Center for World Mission today we received this URGENT PRAYER e-mail for David and Michelle Allen, missionaries to Thailand. I think they are with MAF. Their e-mail address snadvm@flash.net.>817-451-0257 (I assume the numbers are a phone number: THE NEED FOR PRAYER David has an invasion of his body by unknown pasasites and apparently only has 2 months to live if God does not intervene. He is a young missionary to Thailand with a 4 month old daughter, Brianna. He comes from a long line missionary family. Please lift David and his family before the throne of grace. Below is quoted from David: "My condition is quite serious now. The body is beginning to break down because I have no more fat or nutrient reserves. My diet consists mostly of vegebroth, gatorade, and saltines. I tried homemade bread a few weeks ago and ended up in the emergency room. I am in constant pain and have to take pain killers regularly. In the last 3 days there have been sharp pains in both kidneys. So far, eight Drs. have not been able to diagnose the parasites. So they are now running tests to see if my kidneys are infected.... They have found two foreign agents, but no one can ID them. One of the effects is to prevent my GI tract from absorbing nutrition. CDC in Atlanta is 3-6 months behind, so they can not help in time. The Dr's. are trying everyone else- they have pictures of the parasite in circulation, trying to get it identified. I am not doing well. I feel like I am in a very dark valley right now. I have been praying for so long for help with no response, that I have become discouraged in prayer. This is a first for me in my life. My prayers are now very elemental:"Father save me!" But the pain continues each day, and I continue to lose weight (lost over 30# to date). Please pray not only for my body but for my spirit. I have not known fear like this before. I don't want to be fearful, and I don't need to be fearful because I am confident in my salvation. I think my fear is related more to the thought of not being with my wife and new baby. This was the happiest time in my life before I became sick." Do you know anyone who can offer David help? Perhaps you can forward this e-mail to someone who is knowlegeable in the area of parasitic disease. I would appreciate any help you can offer. Thank you David J. Mike From Keith.Cienkus at add.ssw.abbott.com Tue Jun 3 13:47:19 1997 From: Keith.Cienkus at add.ssw.abbott.com (Cienkus,Keith) Date: Mon Mar 7 07:30:00 2005 Subject: Veterinary Immunology Message-ID: <0017000001005968000002L082*@MHS> Does anyone know of information relating to complement testing of goats and/or know of any companies that perform this type of testing? My e-mail address is cienkkr.add@notes.abbott.com From KeldS at uic.edu Tue Jun 3 03:45:43 1997 From: KeldS at uic.edu (Keld Sorensen) Date: Mon Mar 7 07:30:00 2005 Subject: Q: protein A instead of a secondary Ab References: <33932CC9.3DA@uic.edu> Message-ID: <3393D9B6.18C7@uic.edu> Remember protein A does not like all subclasses, so probably protein G would be preferred. Protien A and Protein G will pick up Fc receptors on cell surfaces - so will intact secondary Ab (most people would use Fab or Fab2 fragments). Keld. From arruda at SVN.COM.BR Tue Jun 3 16:30:00 1997 From: arruda at SVN.COM.BR (sergio arruda) Date: Mon Mar 7 07:30:00 2005 Subject: NK and CD1 Message-ID: <3394C9C5.23E@svn.com.br> Netters, Anyone up there knows about papers or reviews about human NK and CD1 molecules. Thanks for help me arruda@svn.com.br From PERLA at MAILBOX.HSCSYR.EDU Tue Jun 3 17:48:57 1997 From: PERLA at MAILBOX.HSCSYR.EDU (Andras Perl) Date: Mon Mar 7 07:30:00 2005 Subject: POSTDOCTORAL POSITIONS Message-ID: RESEARCH POSITIONS APPLICATIONS ARE INVITED FOR RESEARCH POSITIONS TO STUDY 1) THE STRUCTURE AND EXPRESSION OF THE HUMAN TRANSALDOLASE GENE AND AN ASSOCIATED HUMAN REPETITIVE ELEMENT (J. BIOL. CHEM. 269: 2847-2851, 1994) 2) OLIGODENDROCYTE-SPECIFIC EXPRESSION AND AUTOANTIGENICITY OF HUMAN TRANSALDOLASE IN PATIENTS WITH MULTIPLE SCLEROSIS (J.EXP.MED. 180:1649-1663, 1994; J. Clin. Invest. 99:123801250, 1997), AND 3) ROLE OF TRANSALDOLASE IN APOPTOTIC SIGNALING (J. BIOL. CHEM, 271:32994-33001, 1996) WITH A SPECIAL EMPHASIS ON OLIGODENDROCYTES AND LYMPHOID CELLS. EXPERIENCE IN MOLECULAR BIOLOGY, IMMUNOLOGY, AND/OR SIGNALING MECHANISMS IS REQUIRED. APPLICANTS SHOULD SEND A LETTER EXPRESSING THEIR INTEREST AND QUALIFICATIONS, A CURRICULUM VITAE, AND THE NAMES, ADDRESSES, AND TELEPHONE NUMBERS OF THREE REFERENCES TO: DR. ANDRAS PERL, ASSOCIATE PROFESSOR OF MEDICINE AND MICROBIOLOGY AND IMMUNOLOGY, STATE UNIVERSITY OF NEW YORK HEALTH SCIENCE CENTER, SYRACUSE, NY 13210, USA From EB15 at le.ac.uk Tue Jun 3 07:26:37 1997 From: EB15 at le.ac.uk (Dr E. Buxbaum) Date: Mon Mar 7 07:30:00 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> Message-ID: <5n12ht$prs@falcon.le.ac.uk> Ali McBride wrote: >Where can I buy the luminol and the p-iodophenol from >Thanks in advance >Ali 4-Iodophenol is available from Aldrich, Luminol from Fluka. Warning: Do not buy Luminol from Sigma, we had a complete failure with that stuff once. From hk-miami at ix.netcom.com Tue Jun 3 20:02:30 1997 From: hk-miami at ix.netcom.com (HK) Date: Mon Mar 7 07:30:00 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> Message-ID: <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> x-no-archive: yes In <5n12ht$prs@falcon.le.ac.uk> "Dr E. Buxbaum" writes: >Ali McBride wrote: >>Where can I buy the luminol and the p-iodophenol from >>Thanks in advance >>Ali >------------------------------------- >4-Iodophenol is available from Aldrich, Luminol from Fluka. Warning: >Do not buy Luminol from Sigma, we had a complete failure with that stuff once. ---------------------------------------------- Maybe it wasn't the luminol...Maybe you mixed or measured something incorrectly, since you said that you only tried it once. I buy Luminol from Sigma...it works just fine. In a side-by-side test of my solutions and the ones in the Amersham ECL kit, my solutions work equally as well. Helene From CURRYBROWN at aol.com Tue Jun 3 22:30:07 1997 From: CURRYBROWN at aol.com (CURRYBROWN@aol.com) Date: Mon Mar 7 07:30:00 2005 Subject: goats and complement Message-ID: <970603232912_677702572@emout09.mail.aol.com> Does anyone have experience with measuring complement in goat sera? Also are there companies and/or universities that have a working complement assay for goats? Rick From maxmass at iol.it Wed Jun 4 15:14:52 1997 From: maxmass at iol.it (Massimo Massaia) Date: Mon Mar 7 07:30:00 2005 Subject: TCR-transfected mature T cells Message-ID: <3395CE9B.18ED@iol.it> Does anybody know what happens if I try to transfect a CDR3-specific sequence in a mature T cells, already expressing a self-rearranged TCR? Does it make sense? Massimo Massaia maxmass@iol.it From synt at IBCH.SIOBC.RAS.RU Wed Jun 4 13:50:42 1997 From: synt at IBCH.SIOBC.RAS.RU (Trakhanova Marina) Date: Mon Mar 7 07:30:00 2005 Subject: Announce: WWW Syntesome GmbH for glycoscientists Message-ID: <3395B9AC.2C2B@ibch.siobc.ras.ru> Dear Colleagues! We have the pleasure to inform you that Syntesome has now a home page (http://www.syntesome.ru/) where you can find information about the following: Reagents for glycobiology Application of neoglycoconjugates in academic and industrial laboratories Custom synthesis of saccharides The company's possibilities in the field of development of assay systems Fields of potential cooperation of Syntesome with pharmaceutical companies in glycotechnology Individual section of this home page are dedicated to: Assay Selectins Glycosyltransferases Generation of antibodies Cytochemistry/Histochemistry Hematology/Blood typing Trasplantation/Xenotransplantation Oncology: markers, targeting, vaccines Virology Neurobiology Study of lectins-receptors and search of their antagonists ------------------------------------------------------------------------------------------------------------------ Syntesome, Gesellschaft fur medizinische Biochemie mbH was founded in 1993 and is active in Glycobiology and Glycotechnology, important and growing fields in life sciences. Our catalogue includes about 200 products, mainly glycoconjugates, which are the result of many years of experience, which our scientific staff has acquired in the academic world. It is the company's ambitious endeavour to provide top performance and to occupy and maintain a leading position in this field. Our commitment is not only to find high level solutions to problems by developing high quality carbohydrate-based biochemicals, but to offer our customers in the scientific world a collection of new and unique products, giving them the possibility to design and perform experiments which were impossible previously. The range of products and services we offer is mainly orientated on the study of the functions of carbohydrates as well as the search and study of carbohydrate-binding proteins in biological processes, and on the discovery of new carbohydrate-mediated interactions. Due to the great flexibility and reproducibility of our synthetic technology, we are in the position to rationaly design and produce new glycoconjugates perfectly suited for each particular applications. We already have a great experience in collaborative research with pharmaceutical companies and academic institutions. At any time we are ready to help our customers in the design and production of a new glycoconjugates, in the development of test-systems for diagnostics and pharmaceutical screening, or in the study of the fine specificity of carbohydrate-binding proteins. --------------------------------------------------------------------- For FREE catalogue mail us synt@ibch.siobc.ras.ru Trakhanova Marina synt@ibch.siobc.ras.ru Syntesome GmbH http://www.syntesome.ru/ From Schmidtl at PPRZ02.HRZ.UNI-MARBURG.DE Wed Jun 4 15:49:27 1997 From: Schmidtl at PPRZ02.HRZ.UNI-MARBURG.DE (Fabian Schmidt) Date: Mon Mar 7 07:30:00 2005 Subject: Surface antigens in fetal mouse tissue Message-ID: <3396215B.3C6@stud-mailer.uni-marburg.de> To whoever can help me : I am looking for a up-to-date overview of the time of appearence and expression of surface antigens in fetal mice. Specially of those involved in the thymic development and the development of paraganglia. I am thankful for every hint. Fabian Schmidt University of Marburg, Germany From yksuen at netvigator.com Wed Jun 4 10:19:54 1997 From: yksuen at netvigator.com (Suen Yick Keung) Date: Mon Mar 7 07:30:00 2005 Subject: Dissolve Zinc Chloride in Cell Culture Medium Message-ID: <01bc70f7$cfe11900$a5628bd0@default> I am going to test the possible inhibitory effect of zinc ions on apoptosis of murine macrophage cell line. It is well known that zinc can inhibit apoptosis in many cell types and I found there are many papers showing reduction of apoptosis of culture cells in the presence of zinc chloride. At the beginning of my experiment, I faced the most fundamental but important problem: the solubility of zinc chloride in neutral pH culture medium. Obviously, the zinc chloride cannot dissolve in the medium when the pH is near neutral (around pH 7.2). No paper (as far as I have obtained) mentioned this (may be it is too simple). And I was unable to find the solution from any chemistry books. Do anyone know the answer ? Please Email to me. Thanks !! Y.K.Suen Email : yksuen@netvigator.com From mandywj at mail.utexas.edu Wed Jun 4 09:55:39 1997 From: mandywj at mail.utexas.edu (WILLIAM J MANDY) Date: Mon Mar 7 07:30:00 2005 Subject: goats and complement References: <970603232912_677702572@emout09.mail.aol.com> Message-ID: <5n3vlb$jbv$1@geraldo.cc.utexas.edu> In article <970603232912_677702572@emout09.mail.aol.com>, CURRYBROWN@aol.com says: Rick: Apparently goats and sheep have problems with unusually high levels of anti-complimentary activity. You may be better off with a different assay if possible. What are you testing? Bill ( mandywj@mail.utexas.edu ) > >Does anyone have experience with measuring complement in goat sera? Also are >there companies and/or universities that have a working complement assay for >goats? > >Rick From EB15 at le.ac.uk Wed Jun 4 12:16:40 1997 From: EB15 at le.ac.uk (Dr E. Buxbaum) Date: Mon Mar 7 07:30:00 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> Message-ID: <5n47to$188@falcon.le.ac.uk> hk-miami@ix.netcom.com(HK) wrote: >Maybe it wasn't the luminol...Maybe you mixed or measured something >incorrectly, since you said that you only tried it once. >I buy Luminol from Sigma...it works just fine. In a side-by-side >test of my solutions and the ones in the Amersham ECL kit, my >solutions work equally as well. I would not say things like this if I hadn't done the ovious tests. In general I had quite a few problems with Sigma's chemicals over the years, for example SDS which was insoluble in water, inactive enzyme... Looks like a quality control problem to me. From o.h.brekke at bio.uio.no Wed Jun 4 08:57:05 1997 From: o.h.brekke at bio.uio.no (Ole Henrik Brekke) Date: Mon Mar 7 07:30:00 2005 Subject: Post Doc position Message-ID: <5n3s7h$2ie$1@ratatosk.uio.no> UNIVERSITY OF OSLO Postdoc position in Immunology/Cell biology (up to 3 yrs) EC-TMR Postdoc position for EC citizen and associated states. Participation in TMR Research Networks: "Intracellular mechanisms of antigen processing and presentation by the MHC Class I and Class II molecules" and "Sorting of endosomal and lysosomal proteins by molecular machineries" ERBFMRXCT960069 og ERBFMRXCT960058 (http://www.cordis.lu/tmr/home.html). Possible research visit to several collaborating laboratories. Salary: aprox. 3000 ECU/mth Only citizens of EC member state + Iceland and Israel. Further info and applic.: Dr. Oddmund Bakke, Dep. of Molecular Cell Biology, Box 1050, University of Oslo, N- 0316 Oslo. email: obakke@bio.uio.no. Phone +47 22855787 fax: +47 22854605 From o.h.brekke at bio.uio.no Wed Jun 4 08:51:24 1997 From: o.h.brekke at bio.uio.no (Ole Henrik Brekke) Date: Mon Mar 7 07:30:00 2005 Subject: Post Doc position in Norway Message-ID: <5n3rss$2hd$1@ratatosk.uio.no> UNIVERSITY OF OSLO Postdoc position in Immunology/Cell biology (up to 3 yrs) EC-TMR Postdoc position for EC citizen and associated states. Participation in TMR Research Networks: "Intracellular mechanisms of antigen processing and presentation by the MHC Class I and Class II molecules" and "Sorting of endosomal and lysosomal proteins by molecular machineries" ERBFMRXCT960069 og ERBFMRXCT960058 (http://www.cordis.lu/tmr/home.html). Possible research visit to several collaborating laboratories. Salary: aprox. 3000 ECU/mth Only citizens of EC member state + Iceland and Israel. Further info and applic.: Dr. Oddmund Bakke, Dep. of Molecular Cell Biology, Box 1050, University of Oslo, N- 0316 Oslo. email: obakke@bio.uio.no. Phone +47 22855787 fax: +47 22854605 From dseegert at aol.com Wed Jun 4 15:40:31 1997 From: dseegert at aol.com (Dirk Seegert) Date: Mon Mar 7 07:30:00 2005 Subject: Wanted: TNFa knock out macrophages Message-ID: <5n3k16$sb2$2@news.fhg.de> Hi there, I'm involved in studying regulatory mechanisms of TNFa expression. For this pupose I have made some TNF construct which I want now to transfect into macrophage cellines to see whether they are inducible by different stimuli. What I'm looking for are one or more human macrophage cellines (THP-1 or U937 prefered) which are not able to produce TNFa after stimulation with LPS or IFNg. Is anybody able to provide me with such cells. May be a collaboration is possible!? Looking forward for your answer Dirk Seegert, Ph.D. Fraunhofer Institute Dept. Immunobiology Nikolai-Fuchs-Str. 1 D-30625 Hannover Fax: +49 (511) 5350 155 Tel. +49 (511) 5350 255 From ayelod at post.tau.ac.il Wed Jun 4 04:43:49 1997 From: ayelod at post.tau.ac.il (oded singer) Date: Mon Mar 7 07:30:00 2005 Subject: X-gal assay for lymphocytes Message-ID: <5n3dcl$2l0@mserv1.dl.ac.uk> I am trying to express beta-gal in lymphocytes. I am using a plasmid that contains the beta-gal gene attached to CMV promotor and this plasmid was electroporated into a T-lymphocytes cell line (JJhan). This plasmid was expressed efficiently in non-lymphoid cell line. I could not detect any beta-gal activity in the T-lymphocytes using the X-gal staining method. Does any one know about any successful X-gal assay protocol for lymphocytes or any reason why this assay is not working in lymphocytes? I must say that we are using electroporation (gene pulser) routinely to transfect T-lymphocytes with plasmids and transfected plasmid DNA can be easily detected in southern blott. Thanks, Oded From pnavarro at inav.net Tue Jun 3 23:19:09 1997 From: pnavarro at inav.net (Pedro A Navarro) Date: Mon Mar 7 07:30:00 2005 Subject: RPA kits from Ambion Message-ID: <01bc709e$baca6020$e46b78c7@pnavarro.inav.net> Does anybody know if Ambion's "Direct Protect Kit" offers any real advantages over their RPA II kit, or any comparable RPA kit? I wonder if it works on all the tissues. My research involves working on blood, liver, and lymphoid organs. The idea of not having to isolate the RNA prior to the assay seems attractive, but I wonder if there is any downside to this. Thanks for your time! _ _____________________________________ _ / ) | Pedro A Navarro | ( \ _( (_ | The Univ.of Iowa, Micro Dept. | _) )_ (((\ \>|_/->_____________________________<-\_| Message-ID: <339578C8.5F2C@whoi.edu> David Aldridge wrote: > *Luminol 4mg/ml in DMSO 1ml > *p-iodophenol 1mg/ml in DMSO 1ml > 1M Tris.HCL pH 7.5 0.6ml > H2O2 (30%) 5 microL > H2O 7.5ml > > *Store at -20 > Mix this stuff up fresh a few minutes before use. > Works for me! I assume this is for HRP, does it work for AP, too? If not, does anybody have one for AP? Eli Hestermann -- Eli V. Hestermann ehestermann@whoi.edu Woods Hole Oceanographic Institution Massachusetts Institute of Technology "Vita brevis est, ars longa" - Seneca From knecht at uconnvm.uconn.edu Wed Jun 4 12:54:43 1997 From: knecht at uconnvm.uconn.edu (David Knecht) Date: Mon Mar 7 07:30:01 2005 Subject: Macrophage or neutrophil chemotaxis to fMLP Message-ID: <3395ABB4.13B@uconnvm.uconn.edu> We are trying to repeat some old neutrophil chemotaxis experiments with neutrophils and macrophages responding to fMLP. We have isolated mouse peritoneal cells before and after thioglycolate injection adn then simply looked for random motility stimulated by uniform fMLP in buffer or media. The cells come out very round and rarely attach to glass or plastic, spread or move before or after fMLP addition. Is there some trick we are missing here that gives activatable cells? Thanks, Dave From marann at bconnex.net Wed Jun 4 19:48:59 1997 From: marann at bconnex.net (Dr. Martin Nemec) Date: Mon Mar 7 07:30:01 2005 Subject: Seeking post-doc in immunology Message-ID: <01bc7149$155d0420$a90e05d1@martin.bconnex.net> I am looking for my first post-doctoral post. I received my Ph.D. in immunology from the University of Glasgow, Scotland in the summer of 1996. I am interested in all areas of immunology, especially the mechanisms involved in intracellular signaling. I have used a variety of methods for studies at the cell, as well as molecular level. Also, I was involved in development, characterization and application of monoclonal antibodies. I have extensive experience in computing, including hardware and software setup and programming. For my academic and employment history please see my resume at: http://www.bconnex.net/~marann/mcv.html. Sincerely, Martin Nemec. From syslan2 at net.disbumad.es Thu Jun 5 00:03:24 1997 From: syslan2 at net.disbumad.es (Javier Casas Ciria) Date: Mon Mar 7 07:30:01 2005 Subject: Chlamydia pneumoniae and atherosclerosis Message-ID: <3396489C.15ED@net.disbumad.es> READ THE FULL TEXT ARTICLE The Link Between Chlamydia pneumoniae and Atherosclerosis ------------------------------------------------------------- There is a growing body of evidence suggesting that coronary artery disease is caused by an infectious agent. The leading candidate is Chlamydia pneumoniae, based on seroepidemiologic and histopathologic studies. in this page: When the representative articles on this topic are analyzed surprising data and apparently contradictory can be found. A datum that Saikku et al. considered fundamental and carries to them to establish this association, is that the patients with acute myocardial infarction or with coronary hearth disease were presenting geometric average of IgG greater than control group (P. Saikku, 1998). However, in 1993, Kuo et al demonstrate the presence of C. pneumoniae through PCR and electron microscope in plaques of atheroma from coronary. Precisely in those that previously to their death were presenting low titles of IgG or absence (C.C. Kuo, 1993). This apparent discrepancy seems very difficult to conciliating through clinic experiment and perhaps alone could now to speculate as of indirect data to explain these publications. A possible explanation would be in the immune capacity of the body against to the systemic infection by C. pneumoniae. A good immune response to an infection by chlamydia can produce distortion of membranes of chlamydia, losing its typical morphology and in a way aberrant appearance with difficulty identificable to the optical microscope. In this case certainly it will appear in blood antibodies in microimmunofluorescence. If is not produced an adequate immune response chlamydia maintains its morphology and antibodies can be not detected in serum by microimmunofluorescence (R. Malinverni, 1996). On the need noted by Muhlestein of infectious animal models of appearance of atherosclerotic plaque by the infection by C. pneumoniae to consider a causal relationship of C. pneumoniae already it has been published by Fong et al. in Journal of Clinical Microbiology, January of 1997 (I.W. Fong, 1997). On the other hand if we take as certain the causal association of C. pneumoniae with the coronary pathology it should be of outlining the boarding of this problem. A first possibility would be the vaccine. But as occurs with the trachoma and C. trachomatis there can not to solve the problem, since in one moment of the evolution of trachoma, immune stimulation is prejudicial in the evolution of the process. Other possibility would be the antibiotic treatment. On this has been said that upon trying a great infected population resistances can be created to erythromycin or tetracyclines. This phenomenon can not prevent its therapeutical use in this process as either prevents it in trachoma. Just as in trachoma, in advanced and very chronic injuries the antibiotic treatment there can not to alter the evolution of the coronary disease caused by this germ. For this is necessary to make an early diagnosis of the chronicle infection by C. pneumoniae. Thus we should develop a exact diagnostics technique for the diagnosis of the chronicle infection by C. pneumoniae. Malinverni R. 1996. The role of cytokines in chlamydial infections. Curr Opin Infect Dis 9, 150-155. Kuo CC, Shor A, Campbell LA, Fukushi H, Patton DL, Grayston JT. 1993. Demonstration of Chlamydia pneumoniae in atherosclerotic lesions of coronary arteries. J. Infect. Dis. 167(4):841-9. Saikku p, leinonen m, mattila k, ekman mr, nieminen ms, makela ph, huttunen jk, valtonen v. 1988. Serological evidence of an association of a novel Chlamydia, TWAR, with chronic coronary heart disease and acute myocardial infarction. Lancet. 2(8618):983-6 Fong IW, Chiu B, Viira E, Fong MW, Jang D, Mahony J. 1997. Rabbit model for Chlamydia pneumoniae infection. J. Clin. Microbiol. 1997;35:48-52 From mcpart at nccn.net Thu Jun 5 13:03:17 1997 From: mcpart at nccn.net (Brian P. McPartland, O.D.) Date: Mon Mar 7 07:30:01 2005 Subject: Is there a way to deminish IL-2 excess, without disrupting T-cell and NK cell activity? From a novice immunologist. Message-ID: <5n6v15$mkt@nccn3.nccn.net> Any ideas? Perhaps via attempts to regulate CD 8+/CD 28 molecules? does this make any sense? Brian From lambu at biochem.iisc.ernet.in Thu Jun 5 11:58:05 1997 From: lambu at biochem.iisc.ernet.in (Ashok M S) Date: Mon Mar 7 07:30:01 2005 Subject: hemophagocytosis Message-ID: <5n6r6t$88o@mserv1.dl.ac.uk> hi, i would like to know what is hemophagocytosis. any reference about it or the protocol of it would be helpful. thankyou pl reply to george@biochem.iisc.ernet.in or by newsgroup. karthik dept of biochemistry indian institute of science bangalore india From ans033 at sysc.abdn.ac.uk Thu Jun 5 10:45:45 1997 From: ans033 at sysc.abdn.ac.uk (m.g.blaylock) Date: Mon Mar 7 07:30:01 2005 Subject: IL-4 positive CD3-ve cells Help? Message-ID: <5n6mv9$221@info.abdn.ac.uk> I have been doing some flow cytometry on T cells and i am trying to find the balance of T helper cell one and two subgroups by measuring the amount of il-4 and IFN GAMMA, i have been having trouble getting any IL-4 staining with a FITC anti human IL-4, on CD4 positive cells, so to test my antibody i did the test with a different antibody to IL-4 conjugated with PE, but as i only had a FITC CD3 antibody t wasn't specific for T helper cells. I found i had good staining for IL-4 both on CD3 positive and negative cells, and i am having trouble finding out what group of cells are CD3 negative and produce IL-4 on stimulation. I have gated on lymphocytes only on the flow cytometer so they are not anything else such as mast cells. i would be grateful for any suggestions, and would like to know if any-one else has had trouble getting IL-4 production from activated T cells from healthy volunteers. Anna From assist at soback.kornet.nm.kr Thu Jun 5 05:12:00 1997 From: assist at soback.kornet.nm.kr (Oh Hwa-gyun) Date: Mon Mar 7 07:30:01 2005 Subject: Looking for the source of CD5 and CD3 Message-ID: <01bc7198$c38b8680$17d07ea8@KORNET.kornet.nm.kr> i'm looking for the source of human CD5 and CD3 molecules. i have been anti-human CD5 monoclonal ab. with jurkat cell-line but it was not good immunogen. so, i have found the purified human CD5 and expressed it in the prokaryotic system. badly, Expressed human CD5 was not efficient for the immunogen. i need it , i must get it,,,, please, any comments would be so greatly appreciated ! Hawgyun Oh From sergey at guiness.ksisti.alma-ata.su Thu Jun 5 00:05:26 1997 From: sergey at guiness.ksisti.alma-ata.su (Sinenko Sergey Anatolievich) Date: Mon Mar 7 07:30:01 2005 Subject: New MAB to superantigene Message-ID: Dear audience, We have obtained monoclonal antibody to immobilized ( not free ) superantigen staphilococcal enterotoxin A (SEA). We are interested the research by MAb of action mechanism SEA as immunomodulator (action on NK cells) . We search for to the interested researchers therefore to a question, form of collaboration can be from the granting MAb up to joint Grants. We wait for the proposals. Thank you Sergey Sinenko A. Junior scientific employee 480012, Kazakstan, Almaty, Ajtkhozhin`s Institute of Molecular Biology and Biochemistry (IMBB), 86 Michurin St. Tel. 7(3272)347092, E-male: adm@bioch.academ.alma-ata.su From vblasch at gwdg.de Wed Jun 4 23:13:42 1997 From: vblasch at gwdg.de (Volker Blaschke) Date: Mon Mar 7 07:30:01 2005 Subject: Quantification of T-cell infiltrate Message-ID: <33963CF6.25D9@gwdg.de> Dear fellow researchers, I need to quantify the T-cell infiltrate in biopsies and thought about CD3 as a marker. Now, since I am using quantitative RT-PCR, I am wondering which of the chains is best for this purpose? Are all equally useful? Thanks for the input! Volker -- ------------------------------------------------------------ Dr. Volker Blaschke Dept. of Dermatology vblasch@gwdg.de Georg-August-University Tel. xx49-551-396410 von-Siebold-Str. 3 (then have me paged) D-37075 Goettingen Germany From mdalton at worldnet.att.net Wed Jun 4 23:34:45 1997 From: mdalton at worldnet.att.net (Mark Dalton) Date: Mon Mar 7 07:30:01 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> Message-ID: <339641E5.819@worldnet.att.net> I tried this once it was much less sensitive than super signal from Pierce Chemicals. By the way Pierce is 1/2 the price of ECL from Amersham, and is more sensitive. I suggest you try the homemade stuff for yourself but make sure you do the controls the chemicals were very cheap around $20. I found protocol in the Red Book. It is listed in the other replies. Good Luck Mark Thomas Cameron wrote: > > Company kits end up costing $3-5 per western blot which is 90% of the > cost of the whole procedure. > > People must have some good homemade HRP (and AlkPhos) Chemiluminescent > Detection protocols or references? > > Could you send me one or point me to it? Thanks. > > -- > Thomas Cameron From frank.mullens at mcmail.vanderbilt.edu Mon Jun 2 16:57:35 1997 From: frank.mullens at mcmail.vanderbilt.edu (Frank Mullens) Date: Mon Mar 7 07:30:01 2005 Subject: plasmid DNA prep using CsCl-EtBr Message-ID: <339341CF.7F8D@mcmail.vanderbilt.edu> I have been troubled recently with my plasmid DNA prep using CsCl gradient method. I have noticed that there were significant amount of EtBr stuck to the DNA in some preparations (but not in all cases). EtBr seemed bound to DNA very tight and tolerated repeat extraction using 1-butanol, even when I titrated pH to 10.00 in an attempt to neutralize the positive group on EtBr. I wonder if these molecules somehow covelently bound to the plasmid DNA. I am not certain how these residual EtBr molecules may do to my experiment. Will they mess up my measurement of DNA concentration? cause toxicity once being introduced to cells? Can someone experienced offer me some hint on these questions and how to avoid this problem? Kink From flefever at ix.netcom.com Wed Jun 4 23:05:06 1997 From: flefever at ix.netcom.com (F. Frank LeFever) Date: Mon Mar 7 07:30:01 2005 Subject: Route of immunisation???? References: <338E908D.C9E@alpha2.curtin.edu.au> Message-ID: <5n5dti$bh5@dfw-ixnews5.ix.netcom.com> >>Hello everyone, >> I am pretty confuse here about the route of immunisation!!! I am >>currently working with a mouse model where I take my protein antigen in >>Freund's adjuvant.... and immunised the victim via intraperitoneal route >>.... in the hope of stimulating a good systemic response as well as good >>T-cells reponse.... >> However, I have been receiving advice from people (immunologists) >>and found that ip. immunisation is not a good way to go about if I want >>a good systemic/T-cells reponse..... They all recommended subcutaneous >>injection or intradermal injection.... >> Problem is I am so used to ip. route immunisation and my work have >>been sort of based on ip. immunisation.... and things have not been >>working very well for me... and on the other hand, if I change the route >>of immunisation.. then my work is sort of down the drain!!!! >> Does anyone have any insight to this!!!!????? Please!!!?? > >>Thank you!!!! > >>Sincerely, >>Grace Ho PhD student >>Curtin University of Technology >>Western Australia >>Email: EHO17@alpha1.curtin.edu.au > > >If the only reason you have to continue with i.p. immunization is that you >are used to it, maybe you should review your entire approach to research. > Omar O. Barriga Maybe Omar should review HIS approach to research. My inference is that she does not want to change the route IN THE MIDDLE OF A STUDY. Besides the merely "formal" consideration that one should not change details of procedure from subject to subject, there is the very real possibility that route may affect potency in ways you don't imagine. Don't have the reference right here, but I believe Bruce McEwen (current pres., Society for Neuroscience) was one author on recent paper (POSSIBLY in Proc. Nat. Acad. Sci) on impact of stress on immunization. One route may be more stressful than the other (and not just for the researcher!). Frank LeFever New York Neuropsychology Group From rjjensen at inav.net Wed Jun 4 23:14:27 1997 From: rjjensen at inav.net (Robert J Jensen) Date: Mon Mar 7 07:30:01 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> <5n47to$188@falcon.le.ac.uk> Message-ID: <33963D23.42B2@inav.net> Over the years I have had very good luck with chemicals from Sigma. In my book they are a top notch comapny. rjj From hk-miami at ix.netcom.com Wed Jun 4 19:01:15 1997 From: hk-miami at ix.netcom.com (HK) Date: Mon Mar 7 07:30:01 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> <5n47to$188@falcon.le.ac.uk> Message-ID: <5n4vkb$st0@sjx-ixn4.ix.netcom.com> x-no-archive: yes In <5n47to$188@falcon.le.ac.uk> "Dr E. Buxbaum" writes: > >hk-miami@ix.netcom.com(HK) wrote: > >>Maybe it wasn't the luminol...Maybe you mixed or measured something >>incorrectly, since you said that you only tried it once. >>I buy Luminol from Sigma...it works just fine. In a side-by-side >>test of my solutions and the ones in the Amersham ECL kit, my >>solutions work equally as well. > >I would not say things like this if I hadn't done the ovious tests. In >general I had quite a few problems with Sigma's chemicals over the years, >for example SDS which was insoluble in water, inactive enzyme... Looks >like a quality control problem to me. ----------- I'm not sure what a more oBvious test would be than testing it side by side with the reagents I was trying to duplicate and seeing that both worked equally well. What other test would you suggest? Helene > From swede at biodec.wustl.edu Thu Jun 5 15:42:19 1997 From: swede at biodec.wustl.edu (Marci Swede) Date: Mon Mar 7 07:30:01 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> <5n47to$188@falcon.le.ac.uk> <5n4vkb$st0@sjx-ixn Message-ID: <5n78bb$kdf$1@newsreader.wustl.edu> 4.ix.netcom.com> Some attributes got messed up here--x the complaint was about Sigma's luminol not working. The request was for testing if the failure was for Sigma luminol or some other reason that batch didn't work. HK (hk-miami@ix.netcom.com) wrote: : x-no-archive: yes : In <5n47to$188@falcon.le.ac.uk> "Dr E. Buxbaum" writes: : > : >hk-miami@ix.netcom.com(HK) wrote: : > : >>Maybe it wasn't the luminol...Maybe you mixed or measured something : >>incorrectly, since you said that you only tried it once. : >>I buy Luminol from Sigma...it works just fine. In a side-by-side : >>test of my solutions and the ones in the Amersham ECL kit, my : >>solutions work equally as well. : > : >I would not say things like this if I hadn't done the ovious tests. In : >general I had quite a few problems with Sigma's chemicals over the : years, : >for example SDS which was insoluble in water, inactive enzyme... Looks : >like a quality control problem to me. : ----------- : I'm not sure what a more oBvious test would be than testing it side by : side with the reagents I was trying to duplicate and seeing that both : worked equally well. What other test would you suggest? : Helene : > From u7x11ai at sunmail.lrz-muenchen.de Fri Jun 6 06:16:46 1997 From: u7x11ai at sunmail.lrz-muenchen.de (Dr. Sigrid Nikol) Date: Mon Mar 7 07:30:01 2005 Subject: Seek for an antibody Message-ID: <3397F19E.1387@sunmail.lrz-muenchen.de> Hi ! I am seeking an antibody against the bacterial neo-resistance gene product, usually used in eukaryotic expression vectors for selection in G418. Is there a way to get it ? Your suggestions and comments will be greatly appreciated. Alexander Maier From huckcho at pop.jaring.my Fri Jun 6 09:12:10 1997 From: huckcho at pop.jaring.my (Huckcho) Date: Mon Mar 7 07:30:01 2005 Subject: Website on suspected Coxsackie outbreak in Sarawak, Malaysia Message-ID: <5n95rq$25q@news2.jaring.my> Updates on suspected Coxsackie outbreak in Sarawak, Malaysia is available at http://www.jaring.my/jkns/outbreak/virus1.htm Huckcho From fof1 at chclu.chemie.uni-konstanz.de Fri Jun 6 10:14:53 1997 From: fof1 at chclu.chemie.uni-konstanz.de (Frank O. Fackelmayer) Date: Mon Mar 7 07:30:01 2005 Subject: Dissolve Zinc Chloride in Cell Culture Medium References: <01bc70f7$cfe11900$a5628bd0@default> Message-ID: In article <01bc70f7$cfe11900$a5628bd0@default>, "Suen Yick Keung" wrote: > I am going to test the possible inhibitory effect of zinc ions on apoptosis > of murine macrophage cell line. It is well known that zinc can inhibit > apoptosis in many cell types and I found there are many papers showing > reduction of apoptosis of culture cells in the presence of zinc chloride. > At the beginning of my experiment, I faced the most fundamental but > important problem: the solubility of zinc chloride in neutral pH culture > medium. Obviously, the zinc chloride cannot dissolve in the medium when > the pH is near neutral (around pH 7.2). No paper (as far as I have > obtained) mentioned this (may be it is too simple). And I was unable to > find the solution from any chemistry books. > > Do anyone know the answer ? Please Email to me. > > Thanks !! > > Y.K.Suen > > Email : yksuen@netvigator.com Try zinc sulfate, it dissolves up to 1M (at least). Hope this helps, Frank -- Dr. Frank O. Fackelmayer Division of Biology University of Konstanz D-78434 Konstanz Germany From yksuen at netvigator.com Fri Jun 6 10:47:04 1997 From: yksuen at netvigator.com (Suen Yick Keung) Date: Mon Mar 7 07:30:01 2005 Subject: Anti-DNase I and DNase II Message-ID: <01bc7291$0cc39560$c76b8bd0@default> Hi, Do anyone know any commercial supply of anti mouse DNase I and DNase II antibody ? Thank you for your attentions. Y.K.Suen. yksuen@netvigator.com From internetalias at mayo.edu Fri Jun 6 10:59:24 1997 From: internetalias at mayo.edu (Hongyu Yang) Date: Mon Mar 7 07:30:01 2005 Subject: human T cell lines without TCR Message-ID: <5n9c4s$d6k$1@tribune.mayo.edu> Hi, There. Does anyone know if there is a Human T cell line without the T cell receptor? I want use such a cell line to transfect my desired T cell receptor constructs. The only known Human T cell line without TCR alpha chain is a mutant Jurkat cells. but I don't know if there is a cell without both the Alpaha and beta chain. any comments and suggestions are highly appreciated. Hongyu From dmullins at vt.edu Fri Jun 6 09:30:38 1997 From: dmullins at vt.edu (David Warren Mullins) Date: Mon Mar 7 07:30:01 2005 Subject: interleukin designations Message-ID: What is the offical (Int. Union Imm. Soc.) interleukin designation upto? Is it IL-18? At the AAI meeting in San Fran, some people were referring to IL-18, and others were not stating that it's "not official." Also, can anyone tell me where to locate this information in the future? Many thanks, David ****************************************************************************** David W. Mullins Ph.D. Candidate Microbiology and Immunology Section, Department of Biology Virginia Polytechnic Institute and State University (Virginia Tech) 2119 Derring Hall Blacksburg, VA 24061-0406 voice: (540) 231-8933 fax: (540) 231-9307 http://www.vt.edu:10021/D/dmullins From devvlin at darwin.stanford.edu Sun Jun 8 19:23:59 1997 From: devvlin at darwin.stanford.edu (Brian Devlin) Date: Mon Mar 7 07:30:01 2005 Subject: IL-4 positive CD3-ve cells Help? References: <5n6mv9$221@info.abdn.ac.uk> Message-ID: <339B4D1C.5F00@darwin.stanford.edu> Good staining on CD3 positive and negative? Do you mean that it stains all cells? If thats the case, then something is thouroughly messed up. If you can't see a negative and a positive population for a stain, then its a worthless stain. From devvlin at darwin.stanford.edu Sun Jun 8 19:25:34 1997 From: devvlin at darwin.stanford.edu (Brian Devlin) Date: Mon Mar 7 07:30:02 2005 Subject: Is there a way to deminish IL-2 excess, without disrupting T-cell and NK cell activity? From a novice immunologist. References: <5n6v15$mkt@nccn3.nccn.net> Message-ID: <339B4D7B.3601@darwin.stanford.edu> anti IL-2 antibodies would be the common method (if they are available). From vim at usa.net Sat Jun 7 08:18:08 1997 From: vim at usa.net (Victoria) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! References: <01bc7293$7fcc84c0$LocalHost@jaring> Message-ID: <33995F90.1C17@usa.net> Chau Huang Kuan wrote: > > Dear everybody, > E. Excel international have successfully found a > method of using a drop of blood to check whether you got cancer or not. We > already apply the patent of this machine. Next year, 1998, every hospital > in the world will buy this machine from E. Excel. You will find a label on > that machine " made in E. Excel". Surprise!!! > This is really a amazing news.. Do you know that we can check out the > cancer > before it become serious? How many life we can save? snip... This is wonderful....however blood test for cancer is not new. CA125 is one. In my personal experience they is not always indicative. I had cancer and my blood screens (and pap smears) were all clear. According to the tests, I had no cancer. My reality was, I did have cancer. Victoria vim@usa.net From hkchua at pc.jaring.my Fri Jun 6 21:02:47 1997 From: hkchua at pc.jaring.my (Chau Huang Kuan) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! Message-ID: <01bc7293$7fcc84c0$LocalHost@jaring> Dear everybody, E. Excel international have successfully found a method of using a drop of blood to check whether you got cancer or not. We already apply the patent of this machine. Next year, 1998, every hospital in the world will buy this machine from E. Excel. You will find a label on that machine " made in E. Excel". Surprise!!! This is really a amazing news.. Do you know that we can check out the cancer before it become serious? How many life we can save? Another most important thing is all of our products have been proved that will be useful to prevent cancer. E. Excel has found the main reason of suffering from cancer. It's because our body cannot repair DNA which has been damaged by cancer cells. After the experiment, we found that all of our products have the ability to repair DNA, that means that we can prevent the cancer permanently. E. Excel International : http://www.geocities.com/hotsprings/8854/ Chau Huang Kuan Email: hkchua@pc.jaring.my URL: http://www.geocities.com/collegepark/union/1649/ From rom at hedda.uio.no Sun Jun 8 08:07:01 1997 From: rom at hedda.uio.no (Rolf Melheim) Date: Mon Mar 7 07:30:02 2005 Subject: Allergy Solution Message-ID: <1997060815070190219@xyplex05.uio.no> Allergy Solution Open letter to everyone interested: 15 years ago I became allergic, which for periods has been a hell. The doctor concluded after testing that I was over-sensible to birch, asp and alder - and I went every winter through a desensibilation program with injections of pollen extracts. During the worst allergy season in spring I also had to use antihistamines. But for a years I generally suspected other things to be the main reason for the increase of allergy problems - in many urban societies. Among things I suspected were substances in the food. This spring I have tested myself more thoroughly and has done the following, terrible conclusion: It is the drinking water! When I only drink water I buy on bottles (spring water), even for cooking coffee, and use no water from the tap, every trace of my allergy problem is gone! What's the main culprit I don't know, but one may suspect some chemicals used by the water treatment plants. If these chemicals may reach milk or other foodstufs I don't know. If also asthmatics may be helped by avoiding tap water I don't know, but it should be worth a try! Best regards - with more vitality Rolf Melheim Torvbakkgata 2 C 0550 Oslo Norway rolfme@forsok.vgs.no This far I have got 3 comments: 1. Rolf: That allergies to flouride and other chemicals used to treat public drinking water occure is well documented. Also, I'm not familar with how they process drinking water in Norway, so I don't know what they might be putting in it. Stagger Lee Stagger-lee@usa.net 2. Hi An even more common reason would surely be chlorine? Best wishes Alexa alexa@pcug.org.au 3. Subject: Reply to Allergy Solution Date: Thu, 05 Jun 1997 15:19:55 -0500 From: Jo Ann Faber To: "Rolf Melheim" Thank you for sharing your allergy solution with us. I have not come across any published information linking allergies and asthma with tap water. We have a member of the College in Oslo, Norway, (Kjell Aas, M.D., Voksentoppen Allergy & Asthma Institute) and I am forwarding your open e-mail letter to him. Sincerely, Jo Ann Faber ==================================== American College of Allergy Asthma & Immunology ACAAI Web Page: http://allergy.mcg.edu ==================================== From monk at postech.ac.kr Sun Jun 8 03:50:02 1997 From: monk at postech.ac.kr (Seung woo Lee) Date: Mon Mar 7 07:30:02 2005 Subject: Is there anyone who have an experience of CTL assay with rat lymphocyte ? Message-ID: <339b263a.8256999@news.postech.ac.kr> Hi guys! I had hard times to detect CTL response to specific antigen with rat (Buffalo rat; RT1b haplotype). I'm gonna use the hepatoma cell line, but I think it isn't good idea. So is there anyone who have an experience of CTL assay with rat lymphocyte ? I'll appreciate any idea to solve this problem. Thanks! From blethrow at cats.ucsc.edu Thu Jun 5 13:11:08 1997 From: blethrow at cats.ucsc.edu (Justin Blethrow) Date: Mon Mar 7 07:30:02 2005 Subject: Need diptheria toxin DNA for immunotoxin Message-ID: <3397013B.3FD2@cats.ucsc.edu> Hello all. An associate of mine is trying to make an immunotoxin project work on a shoestring budget. If anyone would be willing to send him DTA sequence, it would save a couple hundred dollars and would be enormously helpfull. Thaks in advance- Justin Blethrow From dennis_goos at mindlink.net Sat Jun 7 15:00:28 1997 From: dennis_goos at mindlink.net (dennis_goos@mindlink.net) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! References: <01bc7293$7fcc84c0$LocalHost@jaring> Message-ID: <3399badd.150314024@news.mindlink.net> "Chau Huang Kuan" wrote: >:|Dear everybody, >:| E. Excel international have successfully found a >:|method of using a drop of blood to check whether you got cancer or not. We >:|already apply the patent of this machine. Next year, 1998, every hospital >:|in the world will buy this machine from E. Excel. You will find a label on >:|that machine " made in E. Excel". Surprise!!! >:| This is really a amazing news.. Do you know that we can check out the >:|cancer >:|before it become serious? How many life we can save? >:| Another most important thing is all of our products have been proved that >:|will be useful to prevent cancer. E. Excel has found the main reason of >:|suffering from cancer. It's because our body cannot repair DNA which has >:|been >:|damaged by cancer cells. After the experiment, we found that all of our >:|products have the ability to repair DNA, that means that we can prevent the >:|cancer permanently. >:| E. Excel International : http://www.geocities.com/hotsprings/8854/ >:| >:|Chau Huang Kuan >:|Email: hkchua@pc.jaring.my >:|URL: http://www.geocities.com/collegepark/union/1649/ >:| >:| >:| And where do we read the peer reviews on this ? From paulroda at epix.net Sat Jun 7 11:55:39 1997 From: paulroda at epix.net (Paul I. Roda, M.D., F.A.C.P.) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! References: <01bc7293$7fcc84c0$LocalHost@jaring> Message-ID: <3399928B.50464246@epix.net> Chau Huang Kuan wrote: > Dear everybody, > E. Excel international have successfully found a > method of using a drop of blood to check whether you got cancer or > not. We > already apply the patent of this machine. Next year, 1998, every > hospital > in the world will buy this machine from E. Excel. You will find a > label on > that machine " made in E. Excel". Surprise!!! > This is really a amazing news.. Do you know that we can check > out the > cancer > before it become serious? How many life we can save? > Another most important thing is all of our products have been > proved that > will be useful to prevent cancer. E. Excel has found the main reason > of > suffering from cancer. It's because our body cannot repair DNA which > has > been > damaged by cancer cells. After the experiment, we found that all of > our > products have the ability to repair DNA, that means that we can > prevent the > cancer permanently. > E. Excel International : http://www.geocities.com/hotsprings/8854/ > > Chau Huang Kuan > Email: hkchua@pc.jaring.my > URL: http://www.geocities.com/collegepark/union/1649/ Wonderfull, another source of shoulder pork and ham on this net. First of all, DNA is not damaged by cancer cells, but damage to DNA is just one mechanism that leads to cancer. Other mechanisms include defects in inherited genes which modulate cell growth (oncogenes such as p53 and bcr -1), enviromental signals that cause metaplasia (tobacco, fat -- derived estrogens), and failure of senescent cells to undergoe apoptosis (programmed cell death). As far as a single test to detect cancer -- 30 years ago CEA was thought to be the test. It's helpful in known cancer cases, but isn't sensitive enough to be used as a screen. Ten years ago, a researcher published magnetic resonance data (never confirmed). Five years ago, everyone thought that CA-125 screening could detect early ovarian cancer. However, anything which irritated the pelvis could cause a mild elevation while a higher cutoff misses some early cases. In other words, I doubt you have the "gold standard" single test for cancer (and yes, I checked out your web page. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/immuno/attachments/19970607/198b194a/attachment.html From devvlin at darwin.stanford.edu Sun Jun 8 19:38:46 1997 From: devvlin at darwin.stanford.edu (Brian Devlin) Date: Mon Mar 7 07:30:02 2005 Subject: Route of immunisation???? References: <338E908D.C9E@alpha2.curtin.edu.au> Message-ID: <339B5091.74E7@darwin.stanford.edu> "victim"? From paris at merck.com Mon Jun 9 12:18:52 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: Need help! for parisitic illness in Thailand References: <5n01sf$4q0@dfw-ixnews6.ix.netcom.com> Message-ID: <339C3AFC.42DA@merck.com> > David J. Mike I would also consider animal parasites in this case since it does happen to be thailand. Have him also contact a veterinarian that may be able to look into these parasites. Most parasites can also cross over into humans and may have been entered through the GI tract (eaten) if he has GI problems. Have the doctors or vets consider nematodes such as Haemonchus, Trichostrongylus, or Ostertagi which are common to Cattle. Since it doesn't seem to be in his lungs I would rule out any dog or cat parasites. Anyway its a suggestion. Paris The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From paris at merck.com Mon Jun 9 12:11:20 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: OUTBREAK OF ACUTE VIRUS INFECTION IN SARAWAK, MALAYSIA. References: <33934871.4A65@pc.jaring.my> Message-ID: <339C3938.493A@merck.com> > > Please contact : > > Sarawak Health Department > Email : JKNS2@po.jaring.my > Tel: 60-82-256566 Fax: 60-82-424959 Were the children located in any type of damp living conditions, that would preclude mold growth? There was an outbreak similar to what you describe reported here in the US in Ohio, it turned out not to be viral but rather a mold that was present in the homes of these children. Adults were not affected only growing children between the ages of 2 months and 5 years, and not all within the same radius. Could be a lead that you may not have thought about. Good luck, Paris The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From paris at merck.com Mon Jun 9 12:37:19 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! References: <01bc7293$7fcc84c0$LocalHost@jaring> Message-ID: <339C3F4F.1CF2@merck.com> After the experiment, we found that all of our > products have the ability to repair DNA, that means that we can prevent the > cancer permanently. > E. Excel International : http://www.geocities.com/hotsprings/8854/ > > Chau Huang Kuan > Email: hkchua@pc.jaring.my > URL: http://www.geocities.com/collegepark/union/1649/ Out for the Quik Buck, aren't we? Hmmmmmm........ The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From nobody at cc.unp.ac.za Mon Jun 9 09:01:08 1997 From: nobody at cc.unp.ac.za (nobody@cc.unp.ac.za) Date: Mon Mar 7 07:30:02 2005 Subject: Wanted: anti rat IgE / anti rabbit IgE Message-ID: Hi all, I would be most grateful if somebody would be willing to part with some of their anti-rat-IgE or anti-rabbit-IgE. Please let me know if you have some availible. Many thank's in advance, Rory Morty e-mail: MortyR@biochem.unp.ac.za From name at see.message.body Mon Jun 9 11:11:28 1997 From: name at see.message.body (Nigel C. Eastmond) Date: Mon Mar 7 07:30:02 2005 Subject: LPS -> IFN-g? Message-ID: <339C2B30.4EA@see.message.body> Hi, I have a problem with some of my expts, and am wondering if anyone can help. Unfortunately, it would be foolish of me to detail any protocols here, however some insight into one key question would be of enourmous help: Let's suppose you administered ip. LPS. Would you expect to get an rise in plasma/peritoneal IFN-g? If anyone wishes to answer this question in a more interactive way, then please email me directly as instructed below. Yours, Nige. -- Nigel C. Eastmond, Dept. Pharmacology, University of Liverpool. WWW: http://www.liv.ac.uk/~nce/> Mail: nce@liv.ac.uk If you took all the nephrons in your kidneys, and laid them out across the college quad', you'd be dead. From paris at merck.com Mon Jun 9 12:29:54 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: goats and complement References: <970603232912_677702572@emout09.mail.aol.com> <5n3vlb$jbv$1@geraldo.cc.utexas.edu> Message-ID: <339C3D92.44A5@merck.com> WILLIAM J MANDY wrote: > > In article <970603232912_677702572@emout09.mail.aol.com>, CURRYBROWN@aol.com says: > > Rick: Apparently goats and sheep have problems with unusually high > levels of anti-complimentary activity. You may be better off with a > different assay if possible. What are you testing? > > Bill ( mandywj@mail.utexas.edu ) > > > > >Does anyone have experience with measuring complement in goat sera? Also are > >there companies and/or universities that have a working complement assay for > >goats? > > > >Rick I believe the University of Colorado is working with goat sera. The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From paris at merck.com Mon Jun 9 12:24:37 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: Veterinary Immunology References: <0017000001005968000002L082*@MHS> Message-ID: <339C3C55.30F@merck.com> Cienkus,Keith wrote: > > Does anyone know of information relating to complement testing of goats > and/or know of any companies that perform this type of testing? My e-mail > address is cienkkr.add@notes.abbott.com Can I ask what type of complement testing you are inquiring about? I do know of one company that will be working with goats but the testing is done outside at a university. Please respond via newsgroup, Paris and no it isn't merck. The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From hkchua at pc.jaring.my Sun Jun 8 20:04:09 1997 From: hkchua at pc.jaring.my (Chau Huang Kuan) Date: Mon Mar 7 07:30:02 2005 Subject: Founder of Using a drop of blood to check CANCER!!! Message-ID: <01bc7466$ee2c5420$LocalHost@jaring> Dr. Jau-Fei Chen's profile Obtained Bachelor Degree in Microbiology and Chemistry at the age of 19. Obtained Master Degree in Microbiology, with emphasis in Immunology and Biochemistry at the age of 21. Obtained Ph. D. Degree in Microbiology at the age of 26, taught upper immunology 512 for both Master and PhD program in Brigham Young University. Founded E. Excel International at Utah, U.S.A. in 1987. Invited to present research paper at the conference of International Federation of Immunologist in Washington D. C. in 1987 and 1988. Selected to receive the Global Overseas Chinese Best Young Person Award in 1992. Selected to receive Martin De La Cruz Award. The Highest Honor in the sixth International Congress on Traditional and Folk Medicine held in 1992. Selected to receive the Second Annual Model of Overseas Chinese Youth Entrepreneur in 1993. Selected as one of the 100 most influential Chinese in U.S.A. in 1994. Invited to attend "The Asia Outstanding Chinese New Year Eve Dinner" hosted by U.S.A. President Bill Clinton in 1996. Received "outstanding woman of the year" award in 1996. The Senate of California state designated March 8, 1996 as "Jau-Fei Chen's Day". Received first place award in thesis categories in the Third Annual Conference of World Traditional Medicine in 1996. Named as one of Ten Outstanding Young Americans for 1997 by the United States Junior Chamber of Commerce at their annual congress on January 9-11, 1997 in Washington DC. Further Information, please visit Nutritional Immunology Home Page. URL : http://www.geocities.com/hotsprings/8854/ Regards, -- Chau Huang Kuan e-mail: hkchua@pc.jaring.my From monk at postech.ac.kr Sun Jun 8 21:50:47 1997 From: monk at postech.ac.kr (Seung woo Lee) Date: Mon Mar 7 07:30:02 2005 Subject: Is there anyone who have an experience of CTL assay with rat? Message-ID: <339c205e.4790154@news.postech.ac.kr> Hi guys! I had hard times to detect CTL response to specific viral antigen in rat (Buffalo rat; RT1b). The major problem is the target cell. I used the hepatoma cell line of BUF rat, but this cell has high percentage of spontaneous Cr release and I wonder whether this cell has right component such as MHC, accessory molecules etc. Is there anyone who have an experience of CTL assay with rat or give me any idea to solving this problem? Seung woo Lee monk@postech.ac.kr From ghermans at luc.ac.be Tue Jun 10 03:06:00 1997 From: ghermans at luc.ac.be (Guy Hermans) Date: Mon Mar 7 07:30:03 2005 Subject: T cell epitope prediction References: Message-ID: In article , bunce@lincoln.ac.nz (Michael Bunce) wrote: > Does anyone know where I can find some online algorithms to map out possible T > cell epitopes from a primary sequence? I have found Epiplot, but would like > to get hold of TSites and some other programs that are mentioned in the > literature. I sent an e-mail to a few groups concerning Tsites a few weeks ago. I'll mail you a copy on private e-mail - I don't want to overload usenet servers all over the planet with big attachments. By the way, there is a WWW site (www.dejanews.com) that will locate 'old' e-mail concerning virtually any topic. You can even check the author's e-mail profile! A little bit too big brother to my taste, but helpfull if you want to know who you're dealing with. And no, I'm not connected to these people. But this site would have helped you - that's all I wanted to say. The T-sites program is on it's way to you right now. Only Mac format available, no source code available at the moment to cross-compile, unfortunately. See you, Guy -- Guy Hermans, PhD student Ms research Unit Immunology research group Dr. L. Willems-Institute Dept. of Physiology, LUC University Campus University Campus B-3590 Diepenbeek B-3590 Diepenbeek Belgium Belgium Voice ++32(0)11/26.92.07 Fax ++32(0)11/26.92.09 From jalcorn427 at aol.com Tue Jun 10 11:22:53 1997 From: jalcorn427 at aol.com (JAlcorn427) Date: Mon Mar 7 07:30:03 2005 Subject: Need anti-protein kinase G Message-ID: <19970610162200.MAA12647@ladder02.news.aol.com> I am currently conducting follow up experimentation on the effects of testosterone on corpus cavenosal SMC. I would like to do western blot analysis if I can obtain Ab. Please e-mail me with any information. Thank you, John From fclement at allserv.rug.ac.be Tue Jun 10 06:19:28 1997 From: fclement at allserv.rug.ac.be (frederic clement) Date: Mon Mar 7 07:30:03 2005 Subject: Wanted:KCG Message-ID: <339D383E.6481@allserv.rug.ac.be> Can anyone tell me where to buy Kathon CG (a bacteriocide) ? Please, mail me on this address: evkersch@allserv.rug.ac.be From bunce at lincoln.ac.nz Tue Jun 10 14:20:12 1997 From: bunce at lincoln.ac.nz (Michael Bunce) Date: Mon Mar 7 07:30:03 2005 Subject: T cell epitope prediction Message-ID: Does anyone know where I can find some online algorithms to map out possible T cell epitopes from a primary sequence? I have found Epiplot, but would like to get hold of TSites and some other programs that are mentioned in the literature. Thanks in advance, Mike Bunce AVSG Lincoln University New Zealand Email: Bunce@lincoln.ac.nz From kshreder at znet.com Tue Jun 10 17:01:26 1997 From: kshreder at znet.com (Kevin Shreder) Date: Mon Mar 7 07:30:03 2005 Subject: The Antibody Resource Page Message-ID: <339DCEB6.4303@znet.com> The Antibody Resource Page (ARP) has recently moved to a new URL. The ARP is divided up into 7 sections: 1. Educational Resources - links to pages on antibodies that will interest the novice and expert alike 2. Online Databanks and Databases - links to scientific databases in the area of sequence analysis and hybridoma work 3. Online Journals 4. How to Find an Antibody - a section for ways to find commercial sources (online or otherwise) of antibodies. If you are a researcher who works with antibodies, you cannot afford to miss this section. 5. Online Companies - a large list of online companies (over 90) that sell antibodies or antibody related products. There is also a section for companies that are not online. 6. Miscellaneous - links to various immunological and biotechnology webpages 7. Antibody Gallery - a new addition to the ARP. This is a section where researchers can donate pictures of antibodies for educational purposes. If you have something to donate, please contact me. The ARP is designed for both beginners and experts who are looking for information about antibodies. I am always looking for new links, so if you know of something, please contact me. Or just contact me to let me know what you think of the page! The URL for the Antibody Resource Page is: http://www.antibodyresource.com/ Kevin Shreder, Ph.D. kshreder@znet.com From mkoziel at WEST.BIDMC.HARVARD.EDU Wed Jun 11 00:02:50 1997 From: mkoziel at WEST.BIDMC.HARVARD.EDU (margaret koziel MD) Date: Mon Mar 7 07:30:03 2005 Subject: Euthanasia effects on CTL? Message-ID: <339D6E4E.8E@west.bidmc.harvard.edu> Hello all. I am currently starting some murine experiments after several years of doing only human studies. Specifically, I am interested in immune responses in mice that express a viral protein in the liver. Most of the labs I know use cervical dislocation alone (seems like that's because they have done it for 15 years). Our animal care committee strongly disapproves of cervical dislocation as a method of euthanasia. However, in the course of writing protocols for our animal care committee, I reviewed some of the literature on methods of euthanasia and the effect of different methods on cellular proliferation and CTL assays. I couldn't find much, but a couple of studies showed alterations of both Th and CTL responses when other anesthestics were combined with cervical dislocation (eg methoxyflurane, pentobarb, or CO2). Does anyone have any experience with different methods of murine euthanasia? If there were any differences, were they significant or trivial? Also, since one of the readouts of the experiments is hepatitis, has anyone using methoxyflurane encountered this as a significant problem? Thanks, Margaret Koziel Beth Israel Deaconess Med. Ctr. Boston MA From shelly1811 at aol.com Wed Jun 11 17:37:49 1997 From: shelly1811 at aol.com (Shelly1811) Date: Mon Mar 7 07:30:03 2005 Subject: Urea Breath Test Message-ID: <19970611223701.SAA02512@ladder02.news.aol.com> Hi, I'm a student writting a paper on the UBT (C13). I have found information all over the place as to the sensitivity/specificity. I would like to hear from anyone using the test to let me know of and false positives or the reliability of the test in the low range...2.4 - 4.0. Thanks for your time. shelly1811@aol.com From betts at BOISDARC.TAMU-COMMERCE.EDU Wed Jun 11 13:33:38 1997 From: betts at BOISDARC.TAMU-COMMERCE.EDU (Gordon Betts) Date: Mon Mar 7 07:30:03 2005 Subject: Need a progestin inhibitor Message-ID: <3.0.1.32.19970611133601.006e5e40@boisdarc.tamu-commerce.edu> Does anyone know where I can find RU486 or another progestin inhibitor? thanx gordon ====================================================== J. Gordon Betts, Ph.D. Department of Biological Sciences Texas A&M University - Commerce Commerce, TX 75429-3011 formerly East Texas State University Voice 903/886-5369 FAX 903/886-5991 E-Mail: betts@boisdarc.tamu-commerce.edu ====================================================== From dhavilan at IMM2.IMM.UTH.TMC.EDU Wed Jun 11 12:17:28 1997 From: dhavilan at IMM2.IMM.UTH.TMC.EDU (David L. Haviland, Ph.D.) Date: Mon Mar 7 07:30:03 2005 Subject: Euthanasia Message-ID: <3.0.32.19970611121853.006ecce4@imm2.imm.uth.tmc.edu> At 09:26 6/11/97 -0400, Jeff Frelinger wrote: >Several years ago we did a couple of side by side expts (of course using >different mice) and the metaphane responses seemed to be lower. It was >enough that we were convinced. It is my personal opinion that the ACUC >are usually more concerned with esthetics for the experimenter than >anything else. Somehow sticking a mouse in a jar is better than >breaking their necks. Jeff: I agree with your conclusion as far as results. From jfrelinger at atlas.niaid.nih.gov Wed Jun 11 08:26:12 1997 From: jfrelinger at atlas.niaid.nih.gov (Jeff Frelinger) Date: Mon Mar 7 07:30:03 2005 Subject: Euthanasia Message-ID: <339EA774.7B07@atlas.niaid.nih.gov> Several years ago we did a couple of side by side expts (of course using different mice) and the metaphane responses seemed to be lower. It was enough that we were convinced. It is my personal opinion that the ACUC are usually more concerned with esthetics for the experimenter than anything else. Somehow sticking a mouse in a jar is better than breaking their necks. Jeff Frelinger From brett at BORCIM.WUSTL.EDU Wed Jun 11 01:52:23 1997 From: brett at BORCIM.WUSTL.EDU (brett) Date: Mon Mar 7 07:30:03 2005 Subject: Hot Young *** Sucking Teens * Message-ID: <199706101524.KAA23291@borcim.wustl.edu> >Check out this site, it has tons of Nude Teenagers ****ing >and Sucking ****. > >Are you ready to *** all over a teenagers face? Then Check out: > > > http://www.nasty-schoolgirls.com > > http://www.nasty-schoolgirls.com > > http://www.nasty-schoolgirls.com ALRIGHT, I CALL AGAIN FOR MODERATION. OTHERWISE, I'LL STOP SUBSCRIBING. WHO WANTS THIS GARBAGE DELIVERED *DAILY* TO THEIR MAILBOX?!? Brett Lindenbach Program in Immunology Washington University - St Louis brett@borcim.wustl.edu From janhan at cbs.dtu.dk Thu Jun 12 06:44:55 1997 From: janhan at cbs.dtu.dk (Jan Hansen) Date: Mon Mar 7 07:30:03 2005 Subject: T cell epitope prediction References: Message-ID: <339FE137.6201@cbs.dtu.dk> Michael Bunce wrote: > > Does anyone know where I can find some online algorithms to map out possible T > cell epitopes from a primary sequence? I have found Epiplot, but would like > to get hold of TSites and some other programs that are mentioned in the > literature. > > Thanks in advance, > > Mike Bunce > AVSG > Lincoln University > New Zealand > Email: Bunce@lincoln.ac.nz Please see: Parker tool http://www-bimas.dcrt.nih.gov/molbio/hla_bind/index.html epimatrix tool http://www.epimatrix.com/hiv -- Jan Hansen Center for Biological Sequence Analysis Department of Physical Chemistry The Technical University of Denmark Building 206 DK-2800 Lyngby Denmark Phone: +45 4525 2485 Fax: +45 4593 4808 E-mail: janhan@cbs.dtu.dk WWW: http://www.cbs.dtu.dk/janhan/homepage.html From Oviedo-Orta at cardiff.ac.uk Thu Jun 12 15:37:28 1997 From: Oviedo-Orta at cardiff.ac.uk (Ernesto Oviedo Orta) Date: Mon Mar 7 07:30:03 2005 Subject: Post Message-ID: I am a young MD, with 5 years of experience in immunology research. I looking for a PhD studenship or an immunology related post in UK. I have also experience in Molecular and Cell Biology techniques. CV on resquest. Please if you are interest write me to the following address. Ernesto Oviedo Orta, M.D Medical Research Council Intercellular Signaling Team, Department of Medical Biochemistry, University of Wales Coll. of Medicine, Heath Park, Cardiff CF4 4XN, U.K e-mail: oviedo-orta@cardiff.ac.uk Tel: 01222-747747 Exts: 2284, 2802 Direct Line: 01222-742284, 01222-742802 Fax: 01222-766276 From levy at uab.edu Fri Jun 13 18:02:30 1997 From: levy at uab.edu (David N. Levy) Date: Mon Mar 7 07:30:03 2005 Subject: What HLA type is Jurkat cell line? Message-ID: <5nsji6$7ej@maze.dpo.uab.edu> Anyone know what the HLA type of the T lymphocytic cell line Jurkat is? Thanks. David N. Levy University of Alabama at Birmingham Birmingham, AL 35294-0007 levy@uab.edu From hkchua at pc.jaring.my Fri Jun 13 12:45:19 1997 From: hkchua at pc.jaring.my (Chau Huang Kuan) Date: Mon Mar 7 07:30:03 2005 Subject: Company of using BLOOD to check CANCER!!! Message-ID: <01bc781d$c41dc000$LocalHost@jaring> Professional Achievements of E. Excel International In 1987, Dr. Jau-Fei Chen took her science of Nutritional Immunology one step further by founding E. Excel International, Inc. -- a company specializing in the formulation and manufacture of nutritionally superior herbal food products -- as a way to provide people with the nutrition that they need to maintain a properly functioning immune system. Dr. Chen currently serves as President of E. Excel International, Inc. E. Excel International is a worldwide research and manufacturing company, with facilities and offices around the world. E. Excel's products are the finest in the world, and every formulation has been created not from ancient manuscripts, but from the latest scientific research and technology combined with traditional herbal knowledge. By retesting, rethinking, researching, and then rejecting any shortcomings until every formulation is perfect, E. Excel has developed the most nutritionally excellent products available. The mission of E. Excel is to give the gift of health through its line of products, and the gift of knowledge through continued research in Nutritional Immunology. Each member of the E. Excel research team and staff is dedicated to a high level of excellence, exemplified by none other than Dr. Chen, and to living up to what the company name stands for -- double the excellence! It is not mere happenstance that E. Excel headquarters is located in Springville, Utah. On the contrary, Dr. Chen carefully selected the 15 acre lot on which she built her offices and manufacturing facility because of its pristine environment. Nestled at the base of the breathtaking Wasatch mountains, among the clearest water and freshest air, E. Excel headquarters is located on beautiful wetlands where the land, water, and wildlife are preserved an protected from the pollution of modern day life -- making it one of the choicest locations available. Meticulous care is taken at E. Excel headquarters to manufacture the highest quality products in the industry. The facility conforms to strict governmental regulations, including wall and floor sanitation. The manufacturing facility is separate from all other facilities to ensure purity, and each product is packaged and handled with the utmost care. E. Excel has designed its own, highly technical machinery to produce these products without compromising the essential nutrients inside plant foods. This equipment is also used to ensure the lowest possible microbial exposure levels keeping E. Excel products free from contamination. Daily research is performed in E. Excel's own in-house laboratory. The products are tested at each stage of manufacture to guaranty the highest quality available. Each member of the E. Excel personnel is well-trained and conscientious about their responsibilities. It is by the combined efforts of scientists, engineers, computer specialists, and many others that E. Excel products are produced and distributed. In addition to its extensive manufacturing plant, E. Excel maintains a spacious warehouse and shipping facility to ensure its products will be readily available to meet the global demand. The entire E. Excel product line is the result of years of research performed by Dr. Chen to ensure enhanced immune system function through maximum nutritional benefits. With each passing year, E. Excel continues to expand. It has truly been the vehicle by which Dr. Chen is accomplishing her goal of providing better health to all people. Further Information, please visit Nutritional Immunology Home Page. URL : http://www.geocities.com/HotSprings/8854/ Regards, -- Chau Huang Kuan e-mail: hkchua@pc.jaring.my From derek.gray at surgery.oxford.ac.uk Fri Jun 13 18:16:07 1997 From: derek.gray at surgery.oxford.ac.uk (Derek Gray) Date: Mon Mar 7 07:30:03 2005 Subject: Euthanasia effects on CTL? References: <339D6E4E.8E@west.bidmc.harvard.edu> Message-ID: <33A1D4B7.2BD4@surgery.oxford.ac.uk> margaret koziel MD wrote: > > Hello all. I am currently starting some murine experiments after several > years of doing only human studies. Specifically, I am interested in > immune responses in mice that express a viral protein in the liver. > Most of the labs I know use cervical dislocation alone (seems like > that's because they have done it for 15 years). Our animal care > committee strongly disapproves of cervical dislocation as a method of > euthanasia. However, in the course of writing protocols for our animal > care committee, I reviewed some of the literature on methods of > euthanasia and the effect of different methods on cellular proliferation > and CTL assays. I couldn't find much, but a couple of studies showed > alterations of both Th and CTL responses when other anesthestics were > combined with cervical dislocation (eg methoxyflurane, pentobarb, or > CO2). Does anyone have any experience with different methods of murine > euthanasia? If there were any differences, were they significant or > trivial? Also, since one of the readouts of the experiments is > hepatitis, has anyone using methoxyflurane encountered this as a > significant problem? > > Thanks, > > Margaret Koziel > Beth Israel Deaconess Med. Ctr. > Boston MA Margaret, Will your animal care committee accept CO2 narcosis as an acceptable euthanasia method for mice? This is rapid, (1-2 minutes) appears to cause no suffering and involves no use of synthetic anaesthetic agent. It is accepted by the UK Home Office as a schedule 1 killing method, and I would suspect we have the strictest licencing procedures in the World. Derek Gray Nuffield Department of Surgery Oxford From ptbmb at liverpool.ac.uk Fri Jun 13 05:56:49 1997 From: ptbmb at liverpool.ac.uk (Bernadette Brooks) Date: Mon Mar 7 07:30:04 2005 Subject: CLIP ab Message-ID: <33A12771.5B09@liverpool.ac.uk> Can anyone tell me if there is an antibody available to CLIP ? Thanks From r1623 at erols.com Thu Jun 12 19:47:56 1997 From: r1623 at erols.com (Rick and Marilyn Schuman) Date: Mon Mar 7 07:30:04 2005 Subject: goat complement Message-ID: <33A098BC.4E73@erols.com> Does anyone know of a source of newborn goat complement? Any suggestions appreciated. Thanks, Rick Schuman From plextech at ix.netcom.com Fri Jun 13 22:38:03 1997 From: plextech at ix.netcom.com (Victor Holland) Date: Mon Mar 7 07:30:04 2005 Subject: Beckman Biomek Interface Message-ID: <01bc7873$db553d60$bce1d3c6@dell-laptop> Has anyone had any experience interfacing RS232 serial communication instruments to Beckman Biomek 2000s? I would like to put a ICN Titertek Multidrop on mine. It has a fairly good set of RS232 commands. I just need to figure out how to get the Biomek to talk to it. Any help would be appreciated. Thank you. - Victor plextech@ix.netcom.com From friedr at med.unc.edu Fri Jun 13 22:30:34 1997 From: friedr at med.unc.edu (Randall H. Friedline) Date: Mon Mar 7 07:30:04 2005 Subject: T cell epitope prediction References: Message-ID: <33A2105A.C48F9DDA@med.unc.edu> Michael Bunce wrote: > Does anyone know where I can find some online algorithms to map out > possible T > cell epitopes from a primary sequence? I have found Epiplot, but > would like > to get hold of TSites and some other programs that are mentioned in > the > literature. > > Thanks in advance, > You can try Ken Parker's HLA site: http://bimas.dcrt.nih.gov/molbio/hla_bind/ -- Cheers, Randy friedr@med.unc.edu " I am Homer of Borg. (.sig stolen from someone) Resistance is..........Mmmmmm..donuts!!" From rjjensen at inav.net Sat Jun 14 09:59:51 1997 From: rjjensen at inav.net (Robert J Jensen) Date: Mon Mar 7 07:30:04 2005 Subject: goat complement References: <33A098BC.4E73@erols.com> Message-ID: <33A2B1E7.7BFA@inav.net> Have you tried Pierce Chemical, or Sigma Chemical? From paimm at netgate.net Sun Jun 15 12:13:47 1997 From: paimm at netgate.net (PAIMM) Date: Mon Mar 7 07:30:04 2005 Subject: Symposium: Translation & Stability of mRNA Message-ID: <33A422CB.419B@netgate.net> San Francisco Symposium '97 Translation & Stability of mRNA October 12-14, 1997 Airport Hilton San Francisco, CA USA ORGANIZED BY: Joe Harford PhD National Cancer Institute Michael Katze PhD University of Washington James W. Larrick MD PhD Palo Alto Institute of Molecular Medicine Confirmed Speakers Joel Belasco, New York University Thomas Dever, NICHD Tom Donahue, Indiana University Gideon Dreyfuss, Univ. Pennsylvania Ellie Ehrenfeld, NIH Stan Fields, Univ. of Washington Elizabeth Goodwin, Northwestern Univ. Joe Harford, National Cancer Institute Matthias Hentze, EMBL,Heidelberg Allan Jacobson, Univ. of Massachusetts Michael Katze, Univ. of Washington Jack Keene, Duke University Ruth Lehmann, New York University Lynne Maquat, Roswell Park Cancer Inst. William Merrick, Case Western Reserve Joel D. Richter, Worcester Foundation Jeff Ross, McArdle Laboratory Alan Sachs, UC Berkeley Paul Schimmel, MIT Daniel R. Schoenberg, Ohio State Univ. Robert Singer, A. Einstein College of Med. Nahum Sonenberg, McGill University Palo Alto Institute of Molecular Medicine 2462 Wyandotte Street Mountain View, CA 94043, USA TEL: 415--694-1420; FAX: 415--694-7717 E-mail: paimm@netgate.net www.pano.com/paimm From uncleal at uvic.ca Sun Jun 15 10:18:16 1997 From: uncleal at uvic.ca (Uncle Al Schwartz) Date: Mon Mar 7 07:30:04 2005 Subject: Laboratory Robotics Show References: <01bc7944$c2e5ac20$5c775ecf@dell-laptop> Message-ID: <33A407B8.138@uvic.ca> Andy Zaayenga wrote: > > DON'T MISS IT! Thirty-one vendors will present their latest technologies > and services to The Laboratory Robotics Interest Group on Vendors Night > this Thursday, June 19 from 5:00 to 8:30 pm! Admission is free - and there > will be free hors d'oeuvre courtesy of the vendors. A cash bar will be > available. This is a great opportunity for you to meet with your peers in > the laboratory automation community. Come on out and enjoy an evening with > the LRIG! [massive mercantile snip] What happens to your products' software, firmware, and embedded microprocessesors when the calendar flips to January 2000? Those elegant automated doodads might be a very short-term investment. They tend to track validation and calibration dates, you know. ISO 9002! Not just a screwup, but a perfectly documented SOP FUBAR screwup. -- Alan "Uncle Al" Schwartz UncleAl0@ix.netcom.com ("zero" before @) uncleal@uvic.ca (summer only, cAsE-sensitive!) http://www.ultra.net.au/~wisby/uncleal.htm (Toxic URL! Unsafe for children, Democrats, and most mammals) "Quis custodiet ipsos custodes?" The Net! From tcckd at pc.jaring.my Sun Jun 15 12:44:14 1997 From: tcckd at pc.jaring.my (Tay Chon Chong) Date: Mon Mar 7 07:30:04 2005 Subject: Company of using BLOOD to check CANCER!!! References: <01bc781d$c41dc000$LocalHost@jaring> Message-ID: <01bc79b3$b380c980$e1d58ea1@jaring> Chau Huang Kuan wrote in article <01bc781d$c41dc000$LocalHost@jaring>... > Professional Achievements of > E. Excel International > It is mainly commercialized rather than Nutritional! I known some of the E. Excel International 'representatives' who claim their products can activates human immunity system. Scientific medicines are harmful their are save and can cure all types of illness..... I saw patient's DM deteriorated, hypertension worsening, pocket more empty because money is sucked by them. From tedl at top.spamblock.net Sun Jun 15 18:22:37 1997 From: tedl at top.spamblock.net (Ted Leonard) Date: Mon Mar 7 07:30:04 2005 Subject: Company of using BLOOD to check CANCER!!! References: <01bc781d$c41dc000$LocalHost@jaring> <01bc79b3$b380c980$e1d58ea1@jaring> Message-ID: In article <01bc79b3$b380c980$e1d58ea1@jaring>, "Tay Chon Chong" wrote: > Chau Huang Kuan wrote in article > <01bc781d$c41dc000$LocalHost@jaring>... > > Professional Achievements of > > E. Excel International > > > > It is mainly commercialized rather than Nutritional! > > I known some of the E. Excel International 'representatives' who claim > their products can activates human immunity system. Scientific medicines > are harmful their are save and can cure all types of illness..... > > I saw patient's DM deteriorated, hypertension worsening, pocket more empty > because money is sucked by them. Who gives a damn what your spammed crap is or ain't! Take it out of sci.bio.herp. -- Ted Leonard tedl@top.net http://www.top.net/tedl/standingbear Signature space for rent. Inquire within. From Oz at upthorpe.demon.co.uk Sun Jun 15 15:38:23 1997 From: Oz at upthorpe.demon.co.uk (Oz) Date: Mon Mar 7 07:30:04 2005 Subject: Company of using BLOOD to check CANCER!!! References: <01bc781d$c41dc000$LocalHost@jaring> <01bc79b3$b380c980$e1d58ea1@jaring> Message-ID: In article <01bc79b3$b380c980$e1d58ea1@jaring>, Tay Chon Chong writes > > >Chau Huang Kuan wrote in article ><01bc781d$c41dc000$LocalHost@jaring>... >> Professional Achievements of >> E. Excel International >> > >It is mainly commercialized rather than Nutritional! > >I known some of the E. Excel International 'representatives' who claim >their products can activates human immunity system. Scientific medicines >are harmful their are save and can cure all types of illness..... > >I saw patient's DM deteriorated, hypertension worsening, pocket more empty >because money is sucked by them. > For some strange reason I am not surprised by this post. Whoever would have guessed that Excel Int might not be completely bona-fide? Could it be that real companies with good products don't seem to need to promote their products with self-laudatory posts to newsgroups? No, surely not. -- 'Oz "Is it better to seem ignorant and learn, - or seem wise and stay ignorant?" From zaayenga at lab-robotics.org Sat Jun 14 23:33:11 1997 From: zaayenga at lab-robotics.org (Andy Zaayenga) Date: Mon Mar 7 07:30:04 2005 Subject: Laboratory Robotics Show Message-ID: <01bc7944$c2e5ac20$5c775ecf@dell-laptop> DON'T MISS IT! Thirty-one vendors will present their latest technologies and services to The Laboratory Robotics Interest Group on Vendors Night this Thursday, June 19 from 5:00 to 8:30 pm! Admission is free - and there will be free hors d'oeuvre courtesy of the vendors. A cash bar will be available. This is a great opportunity for you to meet with your peers in the laboratory automation community. Come on out and enjoy an evening with the LRIG! Participating Vendors ---------------------------------------- Ace Glass- Custom Labware Argonaut Technologies, Inc. - Organic Compound Synthesis The Automation Partnership Beckman / Sagian BioDot Inc. (possible) Bohdan Automation Brandel Incorporated Corning / Costar CRS Robotics CyberLab Incorporated Denville Scientific Dynex (formerly Dynatech) - MLX-1000 luminometer EG&G Wallac Incorporated Hewlett Packard Hudson Control Group Inc. InnovaSystems Labman Automation Ltd Matrix Technologies Corporation Millipore Corporation New England Nuclear Packard Instrument Company Perceptive Biosystems Qiagen Robbins Corporation Source For Automation Tecan TomTec TiterTek / Labrepco Incorporated Waters Corporation Whatman Zymark Corporation ------------------------------------------------------- The Laboratory Robotics Interest Group Topical Group of the North Jersey American Chemical Society June 1997 Meeting The officers of the Laboratory Robotics Interest Group are pleased to announce: The Third Annual Vendor's Night Date: Thursday, June 19, 1997 Place: The Morris Room The Embassy Suites Hotel Route 202 Parsippany, New Jersey Time: 5:00 to 8:30 PM Pre-Registration: not required Extensive hors d'oeuvre, courtesy of the vendors, will be available as well as a cash bar. The proceeds from this vendor funded exhibition are also used to finance mailings and pay for various costs of running the group. In this way the LRIG can operate without collecting dues. Please support the group by attending this informative and entertaining meeting. Thirty-one vendors of laboratory automation software and hardware will be present, demonstrating their latest products and services. Last year's Vendor's Night was extremely successful and we hope to surpass that turnout. For vendor registration and more information contact Ed Kanczewski, Vice Chairman, or any of the LRIG officers listed below. There will be rooms available for attendees who wish to stay overnight. Note: If you are receiving this mailer in paper form, please get your email address to us if possible! The Officers: Chairman: Dennis France dennis.france@pharma.novartis.com Novartis (201)503-6030 Vice Chairman: Ed Kanczewski kanczee@aa.wl.com Warner-Lambert (201)540-6479 Secretary: Andy Zaayenga zaayenga@ix.netcom.com Zymark Corporation (908)302-1038 Treasurer: William Haller bhaller@ompus.jnj.com Ortho-McNeil Pharmaceutical (908)218-6341 The LRIG web site is still evolving! Check us out at http://www.lab-robotics.org We offer meeting announcements, a message board, and career opportunities. There are also many links to industry related meetings and conferences, automation web sites, newsgroups, manufacturers, consultants, and our members' companies. Email is becoming very important to us as we try to keep mailing costs down. If you have an email address, please either log on to the web site and leave us a message or send email to zaayenga@ix.netcom.com. DIRECTIONS: Embassy Suites Hotel 909 Parsippany Blvd. Parsippany, NJ 07054 Tel: (201)334-1440 Fax:(201)402-1188 From anastas at MAIL.CYBERLINK.BG Sun Jun 15 15:39:53 1997 From: anastas at MAIL.CYBERLINK.BG (Anastas Pashov) Date: Mon Mar 7 07:30:04 2005 Subject: Route of immunisation???? Message-ID: <33A4514B.7096@mail.cyberlink.bg> Dear Grace, Consider the following "rules": - i.d. -> Th1 - i.p. -> Th2 - CFA -> Th1 - IFA -> Th2 I hope this is of any help. Best regards! Anastas Pashov From Slides at webtv.net Sat Jun 14 20:04:37 1997 From: Slides at webtv.net (Rose Kingsley) Date: Mon Mar 7 07:30:05 2005 Subject: Help! I Got Pinworms From My Kids! Message-ID: <5nvf35$4le$1@newsd-106.bryant.webtv.net> Hello Everyone, I got pinworms while I was teaching a special education class 2 years ago. I have taken Mebendazole, garlic (synthetic and whole cloves) and I still have them. I started my treatments late in my infestation. I did not know what I had for about a year. I just experienced anal itching intermittently for the first year, then it intensified. I started the mebendazole at that point. How can I get rid of these parasites? Has anybody out there tried the product called Clear? Does it really work? Any recommendations on treatments or medications would be appreciated. Please reply by E-Mail, if possible. Address E-Mail to: Slides@WebTV.Net Thank you in advance for any help you can give me. Sincerely, Rose Kingsley From teitelba at aecom.yu.edu Mon Jun 16 08:37:33 1997 From: teitelba at aecom.yu.edu (Rachel Teitelbaum) Date: Mon Mar 7 07:30:05 2005 Subject: Route of immunisation???? References: <33A4514B.7096@mail.cyberlink.bg> Message-ID: More rules: -mucosally administered, Th1 -other routes, Th2 On 15 Jun 1997, Anastas Pashov wrote: > Dear Grace, > > Consider the following "rules": > - i.d. -> Th1 > - i.p. -> Th2 > - CFA -> Th1 > - IFA -> Th2 > I hope this is of any help. > > Best regards! > > Anastas Pashov > > From R.Batrla at DKFZ-Heidelberg.de Mon Jun 16 14:38:35 1997 From: R.Batrla at DKFZ-Heidelberg.de (Richard Batrla) Date: Mon Mar 7 07:30:05 2005 Subject: dendritic cells: MANUAL Message-ID: <33A596A6.7BD7@DKFZ-Heidelberg.de> Hi, does anyone know of a manual for working with dendritic cells? I would be grateful to get these information. Thanx From nightfires at geocities.com Mon Jun 16 10:13:52 1997 From: nightfires at geocities.com (Sigel Bolverk VinDuran) Date: Mon Mar 7 07:30:05 2005 Subject: The Realm Message-ID: <5o3lc5$igg$4@node2.frontiernet.net> Come and visit this great new web site http://www.geocities.com/Athens/Acropoplis/5113/ From jyoung at camelot.bradley.edu Mon Jun 16 15:18:42 1997 From: jyoung at camelot.bradley.edu (James Young) Date: Mon Mar 7 07:30:05 2005 Subject: Acetylcholine antibodies Message-ID: <5o4732$jop@camelot.bradley.edu> Does anyone know where an enterprising research student could acquire some monoclonal antibodies against acetylcholine? Is there even such thing out there? Thanks in advance Jim Young jyoung@camelot.bradley.edu From ttera at libra.bekkoame.or.jp Mon Jun 16 03:51:17 1997 From: ttera at libra.bekkoame.or.jp (Tetsuo Teranishi) Date: Mon Mar 7 07:30:05 2005 Subject: How to obtain the p53 ? Message-ID: <33A4FE82.46FE@libra.bekkoame.or.jp> Please teach me how to obtain the p53 proteins (wild and mutant). 1, Factory or laboratory name 2, Adress, Phone and Fax number From frauwirt at mendel.Berkeley.EDU Mon Jun 16 15:18:49 1997 From: frauwirt at mendel.Berkeley.EDU (Ken Frauwirth BioKen) Date: Mon Mar 7 07:30:05 2005 Subject: CLIP ab References: <33A12771.5B09@liverpool.ac.uk> Message-ID: <5o4739$62k@agate.berkeley.edu> In article <33A12771.5B09@liverpool.ac.uk>, Bernadette Brooks wrote: >Can anyone tell me if there is an antibody available to CLIP ? >Thanks There are two antibodies that I know of which recognize human CLIP: CerCLIP (Peter Cresswell, Yale) detects CLIP free or bound to MHC Class II, but not whole Ii. 30-2 (Alexander Rudensky, UWash - Seattle) detects CLIP only in the context of the murine A(b) molecule. There is some cross-reactivity with murine CLIP, and it can also detect some of the larger Ii fragments (SLIP, LIP) bound to A(b). As far as I know, there is no monoclonal specifically against mouse CLIP, although the epitope for P4H5 overlaps with CLIP (P4H5 can detect whole Ii, but not CLIP-deleted fragments). I believe P4H5 is available from ATCC. Hope that helps, Ken Frauwirth -- Ken Frauwirth (MiSTie #33025) _ _ frauwirt@mendel.berkeley.edu |_) * |/ (_ |\ | http://www.ocf.berkeley.edu/~frauwirt/ |_) | () |\ (_ | \| DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs From michael at demon.demon.co.uk Tue Jun 17 04:40:05 1997 From: michael at demon.demon.co.uk (michael dalton) Date: Mon Mar 7 07:30:05 2005 Subject: Acetylcholine antibodies References: <5o4732$jop@camelot.bradley.edu> Message-ID: In article <5o4732$jop@camelot.bradley.edu>, James Young writes >Does anyone know where an enterprising research student could acquire >some monoclonal antibodies against acetylcholine? Is there even such >thing out there? > >Thanks in advance >Jim Young >jyoung@camelot.bradley.edu > I would have thought the acetyl choline molecule was too small, and too essential for there to be a possibility to make antibodies against them -- michael dalton From fclement at allserv.rug.ac.be Tue Jun 17 06:32:23 1997 From: fclement at allserv.rug.ac.be (frederic clement) Date: Mon Mar 7 07:30:05 2005 Subject: IgM-Elisa Message-ID: <33A675C5.6C02@allserv.rug.ac.be> Hello, Has anyone a good Elisa-protocol for the detection of antigen specific IgM's in human serum ? Is a blocking like BSA needed ? Has anyone experience with very good blockers ? Please, mail me on this address: evkersch@allserv.rug.ac.be From aronsev at post.tau.ac.il Tue Jun 17 08:45:14 1997 From: aronsev at post.tau.ac.il (Evgeny Arons) Date: Mon Mar 7 07:30:05 2005 Subject: tk luc plasmid Message-ID: <5o64da$e4o@mserv1.dl.ac.uk> Hi! Does anybody knows where I can find a map of "tk luc" plasmid? Please e-mail me with any information. Thanks, Evgeny Arons Tel Aviv University From eped at sn.no Tue Jun 17 18:48:19 1997 From: eped at sn.no (Bjoern K. Pedersen) Date: Mon Mar 7 07:30:05 2005 Subject: Antibodies on the NET Message-ID: <33A72243.F40@sn.no> There is a Norwegian company that has recently appeared on the Web: http://www.diatec.com They offer high quality Mabs for research use, and seems to be the first company to base the sales of such products entirely on the Internet. They accept order on the Web, they communicate with you directly through e-mail, they ship by DHL and they supply you Mabs at a reasonable price. So far they have only launched 2 products, but the list of forthcoming products seems promising. I recommend you visit their site! -- - Bj?rn Pedersen From stalib at ubmedg.buffalo.edu Tue Jun 17 11:24:14 1997 From: stalib at ubmedg.buffalo.edu (stalib@ubmedg.buffalo.edu) Date: Mon Mar 7 07:30:05 2005 Subject: Culturing Dendritic cells from CD34+ cells Message-ID: <866560631.10027@dejanews.com> I am a doctoral student at Roswell Park in Buffalo and am currently trying to culture Dendritic cells from CD34+ cells. I am using Iscove's DMEM media supplemented with 10% FBS and the following cytokines, 100ng/ml GM-CSF and stem cell factor and 50ng/ml of TNFalpha. I would like to know what conditions are best for culturing dendritic cells. For eg. media, serum type (human vs bovine), concentration of cytokines used, days of culturing, etc. Also how many cells should I start out iwht and how many cells will I get in the end. Any help is really appreciated. Thanks shaema -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From serge at influenza.spb.su Tue Jun 17 05:40:20 1997 From: serge at influenza.spb.su (Sergey Shevtsov) Date: Mon Mar 7 07:30:05 2005 Subject: immunology Message-ID: subscribe bionet.immunology From cwebster at broombio.demon.co.uk Tue Jun 17 10:06:19 1997 From: cwebster at broombio.demon.co.uk (Craig Webster) Date: Mon Mar 7 07:30:05 2005 Subject: CRP Measurements Message-ID: Is there any utility in measuring CRP levels below 10 mg/L. I believe there are some sensitive assays on the market that make this possible. Are there other comments about CRP measurements in general ? -- Craig Webster |Tel: 01245 514013 Senior Clinical Biochemist |Fax: 01245 514077 Broomfield Hospital |email: cwebster@broombio.demon.co.uk Chelmsford, Essex, UK |http://www.broombio.demon.co.uk/ From g.briars at mailbox.uq.edu.au Tue Jun 17 00:50:57 1997 From: g.briars at mailbox.uq.edu.au (Graham Briars) Date: Mon Mar 7 07:30:05 2005 Subject: Help! I Got Pinworms From My Kids! References: <5nvf35$4le$1@newsd-106.bryant.webtv.net> Message-ID: <5o58k1$dk2$1@nargun.cc.uq.edu.au> In article <5nvf35$4le$1@newsd-106.bryant.webtv.net>, Slides@webtv.net (Rose Kingsley) says: > > > >Hello Everyone, > >I got pinworms while I was teaching a special education class 2 years ago. I have taken Mebendazole, garlic (synthetic and whole cloves) and I still have > >Has anybody out there tried the product called Clear? Does it really work? Any recommendations on treatments or medications would be appreciated. Please r > >Thank you in advance for any help you can give me. > > Sincerely, > > Rose Kingsley > You could try piperazine. It sounds like reinfection. Did you treat your whole household in paralell From martha59 at TERRACOM.NET Mon Jun 16 19:11:19 1997 From: martha59 at TERRACOM.NET (Martha Reilly) Date: Mon Mar 7 07:30:05 2005 Subject: Message-ID: Subscribe immuno From sni1 at le.ac.uk Wed Jun 18 05:25:45 1997 From: sni1 at le.ac.uk (S.N. Imlach) Date: Mon Mar 7 07:30:05 2005 Subject: Why more HLA-DR ? Message-ID: <5o8d39$ka7@hawk.le.ac.uk> Why is more HLA-DR, in general, expressed on the surface of APC. According to my reading so far the same mechanisms for regulating the expression of HLA-DR used for DP and DQ. Does this mean that the promptor activity in DR is increased over the other Class II gene ? Does this overrepresentation of HLA-DR confer any immunological advantage ? Thanks in advance Stuart From sni1 at le.ac.uk Wed Jun 18 05:19:48 1997 From: sni1 at le.ac.uk (S.N. Imlach) Date: Mon Mar 7 07:30:05 2005 Subject: Why more HLA-DR ? Message-ID: <5o8co4$i9o@hawk.le.ac.uk> Why is it that in general more HLA-DR molecules are found on the surface of APC when according to my reading the mechanism for the expression of HLA Class II genes are the same. Does the overrepresentation of HLA-DR confer some advantage to the immune system? Thanks in advance From sni1 at le.ac.uk Wed Jun 18 05:13:26 1997 From: sni1 at le.ac.uk (S.N. Imlach) Date: Mon Mar 7 07:30:05 2005 Subject: Why more HLA-DR Message-ID: <5o8cc6$etj@hawk.le.ac.uk> Does anyone know why HLA-DR molecules are generally expressed at higher levels on From marann at bconnex.net Tue Jun 17 20:34:22 1997 From: marann at bconnex.net (Dr. Martin Nemec) Date: Mon Mar 7 07:30:05 2005 Subject: !Postdoctoral Position Wanted! Message-ID: <01bc7b86$6c048180$7e0e05d1@martin.bconnex.net> I am looking for my first post-doctoral post. I received my Ph.D. in immunology from the University of Glasgow, Scotland in the summer of 1996. I am interested in all areas of immunology, especially the mechanisms involved in intracellular signaling. I have used a variety of methods for studies at the cell, as well as molecular level. Also, I was involved in development, characterization and application of monoclonal antibodies. I have extensive experience in computing, including hardware and software setup and programming. For my academic and employment history please see my resume at: http://www.bconnex.net/~marann/mcv.html. Sincerely, Martin Nemec. From s294039 at ccs.sogang.ac.kr Wed Jun 18 05:54:26 1997 From: s294039 at ccs.sogang.ac.kr (Junhong Min & Sangwoo Lee) Date: Mon Mar 7 07:30:05 2005 Subject: conjugation with IgG & NBD-Cl Message-ID: <33A7BE62.2168@ccs.sogang.ac.kr> Does someone know how to conjugate IgG with NBD-Cl? From otalora at GOLIAT.UGR.ES Wed Jun 18 08:13:51 1997 From: otalora at GOLIAT.UGR.ES (Fermin Otalora) Date: Mon Mar 7 07:30:05 2005 Subject: (none) Message-ID: <199706181314.PAA06011@goliat.ugr.es> The "7th International Conference on the Crystallisation of Biological Macromolecules" will be held in Granada (Spain) during May 3-8, 1998. We hope to see you here to enjoy both the Conference and this exceptional city. Scientific Program will include: =B7 Nucleation behaviour =B7 Growth kinetics and growth mechanisms=20 =B7 Solution studies=20 =B7 Mass transport processes=20 =B7 Mass spectrometry=20 =B7 Molecular engineering=20 =B7 Crystallisation of membrane proteins=20 =B7 2-d crystallisation of proteins=09 =B7 Surface modifications to facilitate crystallisation=20 =B7 Physical properties of protein crystals=20 =B7 Microgravity and related techniques=20 =B7 Design of crystallisation experiments and protocols=20 =B7 Crystal characterisation=20 =B7 Industrial processes=20 =B7 Mosaicity and resolution limit=20 =B7 Colloid crystallisation and protein crystallisation=09 Please contact us for more information Mail: ICCBM 7 Secretariat IACT Campus Fuentenueva (Fac. Ciencias) 18002,Granada (SPAIN)=09 Phone +34-58-243360=09 FAX +34-58-243384=09 Email Iccbm7@ugr.es=09 WWW http://iccbm7.ugr.es/home.html From bostwick at cas.chemistry.gatech.edu Wed Jun 18 12:50:24 1997 From: bostwick at cas.chemistry.gatech.edu (David Bostwick) Date: Mon Mar 7 07:30:06 2005 Subject: RESULT: sci.bio.immunocytochem passes 189:18 References: <865618925.661@isc.org> <864638704.28462@isc.org> Message-ID: <866656222.12938@isc.org> RESULT unmoderated group sci.bio.immunocytochem passes 189:18 There were 189 YES votes and 18 NO votes, for a total of 207 valid votes. There was 1 abstain. For group passage, YES votes must be at least 2/3 of all valid (YES and NO) votes. There also must be at least 100 more YES votes than NO votes. There is a five day discussion period after these results are posted. If no serious allegations of voting irregularities are raised, the moderator of news.announce.newgroups will create the group shortly thereafter. Newsgroups line: sci.bio.immunocytochem Immuno-labelling of biological material. The voting period ended at 23:59:59 UTC, 16 Jun 1997. This vote was conducted by a neutral third party. Questions about the proposed group should be directed to the proponent. Proponent: Amanda Wilson Mentor: Jonathan Grobe Votetaker: David Bostwick RATIONALE: sci.bio.immunocytochem Immunohistochemists and immunocytochemists already enjoy the benefits of online communication, utilizing e-mail, accessing web sites, and subscribing to specialised mailing lists. Usenet newsgroups are also popular, but this is less obvious because articles with immunocytochemical/immunohistochemical content get posted to many different newsgroups. Most articles are posted to a favourite five or six newsgroups including bionet.cellbiol, sci.med.immunology and sci.techniques.microscopy, but often articles get posted to any one of fourteen or fifteen newsgroups in the sci. and bionet. heirarchies. Some of these are listed in the distribution list at the end of this proposal. In my view, no existing newsgroup fulfils the criteria necessary to attract all the various immunocytochemistry postings. I do not wish to draw users away from other newsgroups, only to encourage scientists to share their knowledge and expertise on immunocytochemistry in the most effective manner. Immunocytochemistry and immunohistochemistry are not subdivisions of immunology, molecular biology or chemistry. Microscopy, although essential, is only a small part of the story. Immunocytochemistry and immunohistochemistry are multi- disciplinary, therefore discussions are destined to stay distributed amongst the different newsgroups until they are all brought together under one umbrella. This would then act as a focus point for all the immunocytochemists who are already Internet users, and encourage new subscribers to Usenet. CHARTER: sci.bio.immunocytochem This is a newsgroup for the exchange of information relating to immunocytochemistry and immunohistochemistry, and all forms of related affinity labelling methods, such as lectins and in-situ hybridisation. These unique research tools are used to locate and identify specific molecules in biological material, at the microscopical level. Articles posted to this group must be relevant to one or more aspects of the above. The kind of subjects that may be discussed include techniques, theory, presentation of results, requests for collaboration, history, equipment, publication references, notice of events, tips and trouble-shooting, jobs offered andwanted, jokes, stories and new ideas, so long as the posting bears a direct relevance to the central theme. There will be a list of Frequently Asked Questions (FAQs) to help newcomers. A relevant posting could just be a simple question or answer, for example "Has anyone got any experience with this reagent ?"or "Which course could I attend to learn more about immunogold labelling?". There will be articles reminding people to read the list of FAQs prior to posting their own article. Usenet readers may get involved in complex discussions about, for example, multiple labelling, proper use of control experiments, microwave antigen retrieval or quantitative measurements. Remember that articles posted to a newsgroup are intended for a wide readership, so if you have information which concerns only one or two people then please don't use this newsgroup, use e-mail. Commercial advertisements for services, equipment or reagents violate the charter unless one or more of the following apply: (a)The advertisement is part of a comprehensive article designed specifically to address issues raised in earlier articles posted to the group (b)A general reference to the type of product does not suffice for technical reasons and it is necessary to specify the exact commercial product (c) The information is offered primarily for the benefit of the readers (d)The advertisement is for second-hand equipment specific to immunocytochemistry (e) Requests or offers for free products are acceptable if they are not part of a sales promotion. END CHARTER. DISTRIBUTION: Pointers directing readers to this CFV will be posted in these groups: bionet.diagnostics bionet.molbio.proteins bionet.neuroscience bionet.plants sci.bio.microbiology sci.med sci.med.laboratory sci.misc sci.nanotech sci.bio.immunocytochem Final Vote Ack Voted Yes ------------------------------------------------------------------------------ a8803349@unet.univie.ac.at Martin Offterdinger acalvo@mail2.cti.unav.es acalvo@mail2.cti.unav.es ag414@freenet.carleton.ca Colin Leech ahenry@dircon.co.uk Andrew Henry ajpiekny@acs.ucalgary.ca Alisa J. Piekny akarim@sghms.ac.uk A.KARIM allergy@biochem.iisc.ernet.in P.V.Subbarao anne@cfjf.dyn.ml.org Anne Voice apirkic@zg.tel.hr Ahmed Pirkic ar229@freenet.carleton.ca Allison Haggarty ax041@freenet.carleton.ca Rachelle Leger barryh@norfolk.infi.net Barry H. Hellman, Jr., M.D. baskin@biosci.mbp.missouri.edu Tobias Baskin bbray@netside.com Beth Bray bcelasun@neuron.ato.org.tr Bulent Celasun beebed@am.seer.wustl.edu David C. Beebe belfry@unb.ca Susan Belfry benchaib@rockefeller.univ-lyon1.fr Benchaib Mehdi BERND.BOHRMANN@Roche.COM Dr. Bernd Bohrmann bill.burns@daltile.com Bill Burns bob@play.psych.mun.ca Robert Brown bohmfalk@tcgcs.com John Bohmfalk BoJ@bot.ku.dk Bo Johansen Bonnie3031@aol.com Bonnie Whitaker boutrossw@em.agr.ca Sam Boutros bradt@tmfs.mpgfk.tu-dresden.de Jens Bradt bray@iupui.edu Bruce D. Ray Carol_Bobrowitz.PATHOLOGY@qmail.path.mcw.edu Carol Ann Bobrowitz cbishop@brynmawr.edu Caroline Bishop ccantina@iafrica.com C Cantina cemerson@morgan.ucs.mun.ca Carolyn J. Emerson CHELACK@admin3.usask.ca Brian Chelack childs@mbiweb.utmb.edu Gwen V. Childs, Ph.D. clending@acs.brockport.edu Craig Lending cmrgalle@usc.es Rosalma Gallego Gsmez Colin.Veitch@dwt.csiro.au Colin Veitch d.kiely@ieee.org Don Kiely Decalchem@aol.com Cliff Berger delorme@univ-lyon1.fr Delorme dennis@sydpcug.org.au Dennis Hardgrove deschuyt@sbbio.be Michel Deschuyteneer dfris@primenet.com Dr Sharon M Brookes dgreenwood@hort.cri.nz Dr David R. Greenwood dianavd@eye.usyd.edu.au Diana van Driel dimmicmj@aston.ac.uk Michael Dimmick djab@soton.ac.uk David Brownlee dlazard@netvision.net.il Daniel Lazard dolber@cs.duke.edu Paul C. Dolber drtoad@silcom.com Michae. A. Richardson, M.D. FCAP drtoad@silcom.com edavis@pathserv.Stanford.EDU R. Eric Davis, M.D. elaine.levy@well.ox.ac.uk Elaine Levy elynk@Capital.Net Edgar Lynk em@rz.uni-sb.de Eberhard Morgenstern eric.hines@ento.csiro.au Eric Hines ezudaire@mail2.cti.unav.es Enrique Zudaire fort@ura1195-6.univ-lyon1.fr FORT ganesh@biochem.iisc.ernet.in K.A.GANESH gbza40@udcf.gla.ac.uk Laurence Tetley gkrause@cms.cc.wayne.edu Gary Krause goldmrkr@fast.net Donald P. Cox goode@zool.umd.edu Dennis Goode GOWEN@hera.EMBL-Heidelberg.DE Brent Gowen grbharathan@ucdavis.edu Geeta Bharathan gsanchez@df.uba.ar Gustavo Sanchez gsfraley@vetmed.wsu.edu Gregory S. Fraley gstrout@obsnsrv1.bio.uoknor.edu Greg Strout guymacon@deltanet.com Guy Macon hales@medcor.mcgill.ca Pat Hales hall@aecom.yu.edu David H. Hall Hatton@Ifn-Magdeburg.de Christopher Hatton HeathDA@agresearch.cri.nz Heath, Derek holm@medizin.uni-leipzig.de Max Holzer hraich@marccri.marc.cri.nz Ian Hallett hrapws@marccri.marc.cri.nz Paul Sutherland hstruse@marlin.utmb.edu Dr. H. Struse ivpm26@cc.uab.es Santiago Lopez jacob@uia.ua.ac.be W.A. Jacob jim@hteqa.demon.co.uk Jim Elsam jk09@swt.edu Joseph R. Koke jkiernan@julian.uwo.ca J. A. KIERNAN jklimm@capecod.net John C. Klimm jlah@emory.edu James J. Lah jlitt@capecod.net Gerald J. Litt johna@scientist.com John G. Aghajanian, Ph.D.>=20 jorobins@magnus.acs.ohio-state.edu John M. Robinson josean@mail2.cti.unav.es Jose A. Rodriguez jpo@ipass.net John P. O'Donnell jrs8232@ggr.co.uk John Spaull Judy.Callaghan@med.monash.edu.au judy callaghan judy@playfair.utoronto.ca Judy Trogadis kab35@cornell.edu Kathie Berghorn kjoseph@odont.aau.dk Kaj Josephsen kmackie@u.washington.edu Ken Mackie kmauck@post.its.mcw.edu Kimberly Mauck krerikss@aton.abo.fi Krister Eriksson kshah@uscom.com Keyur Shah kszaruba@mmm.com Karen S. Zaruba lameye@ulb.ac.be AMEYE lani@lava.net Lani Teshima-Miller Laurent.Delepine@esa5017.u-bordeaux2.fr Laurent DELEPINE lesley@unixg.ubc.ca Lesley Weston lesleyjane@ndirect.co.uk Lesley Jane lizard@okway.okstate.edu Ginger R. Baker LYMFNOD@worldnet.att.net Richard Miller m.millar@ed-rbu.mrc.ac.uk Mike Millar MAJOHNS@MKG.COM Michael Johnson making@nervm.nerdc.ufl.edu Michael King marc.espeel@rug.ac.be Espeel mark.baptista@pi.net Mark Baptista mcauliff@UMDNJ.EDU Geoff McAuliffe mcbgu@leonis.nus.sg Gerald Udolph medlab3@leonis.nus.sg Mary Ng Mah Lee mh@nwu.edu Mark Harms millers@em.agr.ca Shea Miller millro29@IDT.NET Rodney T. Miller, M.D. MLKITC@novusint.com MLKITC MLOOTS@medic.up.ac.za Marius Loots MMASSAAD@medcor.mcgill.ca Michel Massaad mmhenson@med.unc.edu Miriam Henson MontagueDonnaC@exchange.uams.edu Donna C. Montague montedeb@montana.campus.mci.net Deborah Berglund Monty_Hyten@bmc.boehringer-mannheim.com Monty J. Hyten mp.rastaldi@mail.asianet.it Maria Pia Rastaldi mroper@globalnet.co.uk Mark Roper msoysal@mistik.express.net Mustafa Soysal MS57 nospam@fwb.gulf.net Jules Dubois Nubsbio@aol.com Noelle Patterson nunns03@msumusik.mursuky.edu Stacey A. Nunn ota@nutr.med.tokushima-u.ac.jp Fusao Ota patph@south-01.novell.leeds.ac.uk Patricia Harnden patra@poseidon.physik.tu-berlin.de Michael Patra paul.webster@yale.edu Paul Webster paxil@inorth.on.ca Trevor Tymchuk pbrunner@u.washington.edu Paulette Brunner phdubois@ulb.ac.be Philippe Dubois Pierre.Aubineau@esa5017.u-bordeaux2.fr AUBINEAU pixeleye@sn.no S. W. Tengelsen PO@stonebow.otago.ac.nz Allan Mitchell r.pitman@zetnet.co.uk RICHARD WILLIAM PITMAN r.sharp@pgrad.unimelb.edu.au Robert ralf@ark.franken.de Ralf W. Stephan rbonshek@fs1.scg.man.ac.uk Richard Bonshek rburry@osu.edu Richard W. Burry rharjula@sun3.oulu.fi Riitta Harjula rhh1@airmail.net Ronald H Houston richard.lander@stonebow.otago.ac.nz Richard Lander rmoss@sghms.ac.uk R.F.MOSS robarias@mail2.cti.unav.es Roberto Arias de Manuel RONALD.COHN@roche.com Ronald Cohn ; ronald.cohn@roche.com rondouc@duke.usask.ca Ronald Doucette roses@capecod.net Dolores Scaldini Klimm rosmanha@pinkinc.com H. A. Rosman rschmitt@discover.net Robert L. Schmitt RSCHOONH.SPH@MHS.UNC.EDU ROBERT SCHOONHOVEN rzs@plantpath.wisc.edu Russell N. Spear samsonw@wadsworth.org William A. Samsonoff savidge@unb.ca Rod Savidge shahn@mail.med.upenn.edu Neelima Shah ShawR@agresearch.cri.nz Richard Shaw simonsmit@aol.com Simon Smith slcritte@facstaff.wisc.edu Sarah Crittenden slipper@net-gate.com Diana G. Goodwin spectrum@pacifier.com Luther DeGado srussell@ou.edu Scott Russell stamper@stamper.com Chris Stamper steffens.b@calc.vet.uga.edu W. L. Steffens strande@UMDNJ.EDU Louise Strande stuart@cosc.canterbury.ac.nz Stuart Yeates t247849@tip.nl Bert Oosting, PhD-student tabone@lyon.fnclcc.fr Eric Tabone terao@bani.ucl.ac.be Terao thomas.hagenloch@student.uni-tuebingen.de Thomas Hagenloch Thorben.Lundsgaard@plbio.kvl.dk Thorben Lundsgaard TOMANDBOB@aol.com Thomas J. Kuwahara ToothDoc@pouch.com Cliff Baynon tpk@rri.sari.ac.uk Tim King tpp3@po.CWRU.Edu Theresa P Pretlow trevarro@uoneuro.uoregon.edu Bill Trevarrow twiadrow@health.adelaide.edu.au Todd Wiadrowski u2174805@acsusun.acsu.unsw.edu.au D Yeung ukaempf@hannover.sgh-net.de Udo Kaempf VANWAGD@cesmtp.ccf.org David Van Wagoner Ph.D. vectorca@marquis.netinc.ca Patrick Keogh virginia@oncology.uthscsa.edu Virginia Boucher vpdura@hiwaay.net Victor Dura W.vanHeumen@vthrc.uq.edu.au WALTER VAN HEUMEN wgschech@med.uni-tuebingen.de Wolfgang Schechinger windoff@goofy.zdv.Uni-Mainz.de Reinhard Windoffer zed@cjnetworks.com Ned Fleming Voted No ------------------------------------------------------------------------------ booda@datasync.com Martin H. Booda darber@smtplink.Coh.ORG Daniel A. Arber euthke@siam.muc.de Ekkehard Uthke gpr96002@uconnvm.uconn.edu G.P. Ryan kimdv@netcom.com Kim DeVaughn legcjjk@_spaamtraap_lusta.latrobe.edu.au Jason King marquez@pacbell.net Aaron Marquez masters@mail.deltanet.com Nacho Masters naddy@mips.rhein-neckar.de Christian Weisgerber olav@viking.mv.com Olav Nieuwejaar patl@lcs.mit.edu Patrick J. LoPresti richsong@vcn.bc.ca Richard Songhurst rick@bcm.tmc.edu Richard Miller stainles@bga.com Dwight Brown steiners@primenet.com Jason Steiner T.Bowden@Queens-Belfast.AC.UK Tony Bowden tonyb@null.net Tony Basoglu una@doliolum.biology.yale.edu Una Smith Abstained ------------------------------------------------------------------------------ kirberg@nki.nl joerg kirberg From Christa.Baumstark-Khan at dlr.de Thu Jun 19 07:58:25 1997 From: Christa.Baumstark-Khan at dlr.de (Christa Baumstark-Khan) Date: Mon Mar 7 07:30:06 2005 Subject: NATO ARW on Fundamentals for the Assessment of Risks from Environmental Radiation Message-ID: <33A92D23.75CE@DLR.DE> ANNOUNCEMENT NATO-Advanced Workshop Fundamentals for the Assessment of Risks from Environmental Radiation October 6-10, 1997 An Advanced Research Workshop of the North Atlantic Treaty Organization (NATO) on Fundamentals for the Assessment of Risks from Environmental Radiation will be held October 6-10, 1997, in Brno, Czech Republic. The purpose of the ARW is (1) to discuss the current state of knowledge of the fundamental radiobiological processes and concepts in risk estimation and radiation protection and (2) to define future research work needed for the assessment of risks to human health and ecosystems from environmental - ionizing as well as UVB - radiation. The review papers, the recommendations and the selected contributions will be published in the NATO Science Series. Limited financial support for participants is available. For further information please contact: Executive Secretary Dr. C. Baumstark-Khan DLR, Institute of Aerospace Medicine Linder H=F6he D-51170 K=F6ln, Germany Phone: +49-2203-601-0 or -3595 Fax: +49-2203-61970 e-mail: christa.baumstark-khan@DLR.de Co-Directors Dr. Gerda Horneck DLR, Institute of Aerospace Medicine 51170 Cologne, Germany Tel.: +49 22036013594=20 Fax: +49 2203 61970 E-mail: Gerda.Horneck@dlr.de Dr. Stanislav Kozubek=20 Institute of Biophysics, Academy of=20 Sciences=20 Kralovopolska 135, 612 65 Brno, Czech Republic Tel/Ans/Fax: +420 5 41240498 E-mail: kozubek@ibp.cz World-Wide Web: http://www.me.kp.dlr.de/Aktuel_info/Nato.html --=20 =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Dr. Christa Baumstark-Khan Inst. F=FCr Luft- und Raumfahrtmedizin/DLR Abteilung Strahlenbiologie Linder Hoehe D-51147 Koeln Tel.: +49 2203 601 3145 Fax: +49 2203 61970 E-Mail: Christa.Baumstark-Khan@DLR.DE =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D From mark_wolcott at nbs.gov Thu Jun 19 15:34:29 1997 From: mark_wolcott at nbs.gov (Mark Wolcott) Date: Mon Mar 7 07:30:06 2005 Subject: Antibodies on the NET References: <33A72243.F40@sn.no> Message-ID: <33A997D5.32A3@nbs.gov> Bjoern K. Pedersen wrote: > > There is a Norwegian company that has recently appeared on the Web: > http://www.diatec.com > They offer high quality Mabs for research use, and seems to be the first > company to base the sales of such products entirely on the Internet. > They accept order on the Web, they communicate with you directly through > e-mail, they ship by DHL and they supply you Mabs at a reasonable price. > So far they have only launched 2 products, but the list of forthcoming > products seems promising. > > I recommend you visit their site! > -- > - Bj?rn Pedersen I'm not sure how a web site with only two products justifies the announcement Bjorn but I'm sure you have you reasons. From scicentral at scicentral.com Fri Jun 13 10:36:39 1997 From: scicentral at scicentral.com (SciCentral) Date: Mon Mar 7 07:30:06 2005 Subject: BEST IMMUNOLOGY DIRECTORIES Message-ID: <33A16907.6216@scicentral.com> Dear Colleagues, We invite you to attend an opening in your honor at: http://www.scicentral.com This unique Web site has been created for scientists and engineers by scientists who know your needs. Just three clicks will get you everywhere. http://www.scicentral.com includes only the most valuable directories on the Web. They currently constitute a gateway to over 50,000 scientific sites pertaining to over 120 specialties in science and engineering ranging from health, biological, earth, physical, and engineering sciences, to government and institutional listings, and including the Commerce Business Daily and Medline--all free. We envision http://www.scicentral.com as the science and engineering hub of the World Wide Web, a place for professionals to gather and discuss the complex issues facing us all as we approach the 21st century. This is where you can make your opinions known. We plan editorials, chat rooms, news. We will continue to be responsive to your suggestions. Please visit http://www.scicentral.com often and bring your colleagues. Ellen S. Uffen, Ph.D. President, SciLink, Inc. Guy Orgambide, Ph.D. Chief Executive Officer SciLink, Inc. Robert L. Uffen, Ph.D Professor Emeritus Michigan State University From aronsev at post.tau.ac.il Wed Jun 18 20:03:50 1997 From: aronsev at post.tau.ac.il (Evgeny Arons) Date: Mon Mar 7 07:30:06 2005 Subject: tk luc plasmid Message-ID: <5oa0hm$bui@mserv1.dl.ac.uk> Hi! Does anybody knows where I can find a map of "tk luc" plasmid? Please e-mail me with any information. Thanks, Evgeny Arons Tel Aviv University From alikadic at emirates.net.ae Thu Jun 19 06:38:12 1997 From: alikadic at emirates.net.ae (Pilot) Date: Mon Mar 7 07:30:06 2005 Subject: Test Message-ID: <33A91A24.16BA@emirates.net.ae> An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/immuno/attachments/19970619/4e5bba66/address.htm From biohelp Fri Jun 20 04:00:05 1997 From: biohelp (BIOSCI Administrator) Date: Mon Mar 7 07:30:06 2005 Subject: BIOSCI/bionet miniFAQ & Fundraiser Message-ID: <199706200900.CAA29812@net.bio.net> (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From ogsdw at ssa.bris.ac.uk Fri Jun 20 10:42:09 1997 From: ogsdw at ssa.bris.ac.uk (S.D. Wainwright) Date: Mon Mar 7 07:30:06 2005 Subject: Detection of antibody with protein-G on blots Message-ID: Dear All Does anybody have a method for the detecting of the primary antibody on western blots using either protein-G HRP or protein-G biotin. I cannot use anti-mouse HRP as I want to identify proteins precipitated by a mouse monoclonal antibody with another mouse monoclonal antibody. Thanks in advance Shane D Wainwright From nigel.osborn at zetnet.co.uk Fri Jun 20 17:22:07 1997 From: nigel.osborn at zetnet.co.uk (Nigel J.Osborn) Date: Mon Mar 7 07:30:06 2005 Subject: Anti idiotype antibodies Message-ID: <5oevqf$m3s$1@irk.zetnet.co.uk> Can anyone out there explain about anti-idiotype antibodies especially antibodies raised against the paratope of an antibody itself raised against hte paratope of an antibody. My understanding is that you can effectively clone proteins this way i.e. they can have the same biological activity as the original protein. From tacollet at tezcat.com Fri Jun 20 13:20:26 1997 From: tacollet at tezcat.com (Thomas A. Collet) Date: Mon Mar 7 07:30:06 2005 Subject: Opinions on Aastrom/In vitro expansion of stem cells? Message-ID: I am considering joining a company called Aastrom. They have technology consisting of a clinical cell culture system for use in the stem cell therapy market. They just completed a clinical study,in which bone marrow cells grown from a small amount of material outside a patient's body (ex vivo) retained the stem and other key immune cells needed to restore vital tissues completely. The implication is that what is an in-patient procedure now can be converted to a much cheaper out-patient procedure. What are the key questions one should ask in assessing the viability of this technology? I do have a biotech background, but have worked in a management mode for the last four years and my science is somewhat rusty. Where else should I post to get an answer? Cheers, Thomas P.S. Please also email me to tacollet@tezcat.com in addition to replying to the posting! From chuckleberry at webtv.net Fri Jun 20 19:54:31 1997 From: chuckleberry at webtv.net (edan portaro) Date: Mon Mar 7 07:30:06 2005 Subject: Why more HLA-DR ? References: <5o8co4$i9o@hawk.le.ac.uk> Message-ID: <5of8o7$6s8$1@newsd-106.bryant.webtv.net> Nature generates mitstakes. What you see as noise is talk: :I am going to the store when really you are staying home. To be mystified by the discoveries of HLA antigens...means you do not think nature can gossip. When you think about survival you should conisder DNA Gossip.... no DNA...no HLA. From stavrosz at med.auth.gr Sun Jun 22 01:43:16 1997 From: stavrosz at med.auth.gr (Stavros P. Zanos) Date: Mon Mar 7 07:30:07 2005 Subject: References in Neuro-Immuno-Endocrinology Message-ID: <33ACC984.5F7@med.auth.gr> Starting at September, I will be participating in a NIE research project, concerning the effects of cytokines in the hypothalamic- pituitary- gonadal glands axis, and I would like to built a minimal background on the field, beyond the standard undergraduate level we are taught in med school. Any introductory or advanced references on this, would be welcome. I already have a good laboratory background in biophysics, cellular neurobiology and neurophysiology-neuropharmacology. Upon receiving an adequate number of replies, I' ll compile a list and post it to the newsgroup. Best regards, and thank you in advance. -- Stavros Zanos Depts. of Experimental Physiology and Neuroradiology Aristotle Univ. School of Medicine Thessaloniki, Greece http://www.med.auth.gr/~stavrosz/index.htm From qball at wickerman.demon.co.uk Sat Jun 21 17:32:45 1997 From: qball at wickerman.demon.co.uk (qball@wickerman.demon.co.uk) Date: Mon Mar 7 07:30:07 2005 Subject: Aspergillus and Nitric Oxide Message-ID: <33ac55e2.408808@news.demon.co.uk> Does anyone know where I might find information on aspergillus and nitric oxide, i.e. macrophage production of nitic oxide to combat aspergillus. I have limited access to journals and need to find some stuff on the web. Any suggestions much appreciated. From John at warbler.demon.co.uk Sun Jun 22 06:40:28 1997 From: John at warbler.demon.co.uk (John Bates) Date: Mon Mar 7 07:30:07 2005 Subject: Antibody Bound Materials Message-ID: <+dss8FAs8QrzEwrE@warbler.demon.co.uk> Can anyone suggest a manufacturer of antibody bound micro-particulate materials (eg microsphers). Any suggestions would be apprecieted. John Bates ******* end of message ****** PHARMACEUTICAL technology Ltd Project Support to the Pharmaceutical and Biotechnology Industries Registered Office : Tamarisk House High Street Colne Huntingdon Cambridgeshire PE17 3ND Tel (44) 01487 740 147 Fax (44) 01487 842 517 Mobile 0468 767 518 E-mail John@warbler.demon.co.uk Web site http://www.warbler.demon.co.uk Director John Bates Ph.D ******* end of signature ****** From LBKing* at mail.med.upenn.edu Mon Jun 23 12:15:47 1997 From: LBKing* at mail.med.upenn.edu (Leslie King) Date: Mon Mar 7 07:30:07 2005 Subject: antibodies to SERCA, MARCKS Message-ID: I am interested in obtaining (either commercially or through a collaboration) antibodies specific for murine MARCKS or murine SERCA isoforms. If anybody knows of a good source, could they please respond to LBKing*@mail.med.upenn.edu (REMOVE THE ASTERISK FROM THE EMAIL ADDRESS FIRST!!) Thanks very much!!! From mnatesan at geocities.com Mon Jun 23 11:26:10 1997 From: mnatesan at geocities.com (Mohan Natesan) Date: Mon Mar 7 07:30:07 2005 Subject: Antibody Bound Materials References: <+dss8FAs8QrzEwrE@warbler.demon.co.uk> Message-ID: <33AEA3A2.F0E4C0E5@geocities.com> Seradyn, Inc. 1200 Madison Ave. Indianapolis IN 46225 800-428-4007 317-266-2991 I think they also have a webpage. Mohan N. John Bates wrote: > Can anyone suggest a manufacturer of antibody bound micro-particulate > materials (eg microsphers). Any suggestions would be apprecieted. > > John Bates > > ******* end of message ****** > > PHARMACEUTICAL technology Ltd > > Project Support to the Pharmaceutical > and Biotechnology Industries > > Registered Office : > > Tamarisk House > High Street > Colne > Huntingdon > Cambridgeshire > PE17 3ND > > Tel (44) 01487 740 147 > Fax (44) 01487 842 517 > Mobile 0468 767 518 > E-mail John@warbler.demon.co.uk > Web site http://www.warbler.demon.co.uk > Director John Bates Ph.D > > ******* end of signature ****** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/immuno/attachments/19970623/436c6624/attachment.html From pjareo at creighton.edu Mon Jun 23 15:17:09 1997 From: pjareo at creighton.edu (Patti W. Jareo) Date: Mon Mar 7 07:30:07 2005 Subject: GTPase assay for low molecular weight GTP binding proteins Message-ID: <33AED9C5.6044@creighton.edu> I am attempting to determine whether the low molecular weight GTP-binding protein I have detected is also exhibiting GTPase activity. I have found numerous references for assays, mostly measuring activity in subunit-type G proteins, however. I am still following up some leads in the literature, but if anyone could point me at a specific assay, it would help tremendously! I do have another question, as well. I repeatedly see that App(NH)p (adenylyimidophosphate) and Gpp(NH)p (guanylylimidophosphate) are used in these assays, and I cannot, for the life of me, figure out WHY one is used rather than the other, or exactly what they are supposed to do! The Gpp... is a nonhydrolyzable GTP analog, and the App... has a similar relationship to ATP. If a nonhydrolyzable ATP analog "plugs up" any ATPases present, I can understand how that would eliminate the potential contribution of any ATPases to the hydrolysis of labeled GTP added as substrate. The GTP analog is supposed to be a Gs activator... but if it is a nonhydrolyzable analog, I'm confused as to what it is supposed to be doing in this reaction! Does anyone know WHY these compounds are used in GTP hydrolysis assays, and how would that impact an assay specifically intended to measure hydrolysis by low molecular weight G proteins rather than the larger subunit type G-proteins?????? I have Sigma fact sheets. I have a ream of papers. ANY help would be greatly appreciated! And I will continue to look, as well.. I'll hit the library again tomorrow morning..... Thanks, folks.... ::sigh:: Patti W. Jareo, Ph.D. Creighton University Omaha NE 68178 From s.harbron at experts.co.uk Tue Jun 24 14:43:42 1997 From: s.harbron at experts.co.uk (Stuart Harbron) Date: Mon Mar 7 07:30:07 2005 Subject: Upgrade your assays with Enzyme Amplification Message-ID: <33b021e8.1300296@news.nildram.co.uk> Up-Grade Your Assays: New Detection We help you get more out of your existing assays, so that you are able to exploit new areas and gain a bigger market share. By replacing existing detection systems with Enzyme Amplification Technology, you benefit from assays which are much faster, or more sensitive, or a combination of both. · It is an impressive, sensitive and rapid assay which can detect as little as 0.03 amol of alkaline phosphatase-labelled analyte in just 20 minutes. · It is ideal for manual and automated assays, and is currently used on Dade’s aca-plus analyser. · It is quantitative, with colorimetric, fluorimetric and luminometric endpoints available. · It is easy to use, having a single-pot assay format - perfect for antibody or gene-probe assays. We have particular expertise in Enzyme Amplification Technology which we helped to develop. This technology is covered by granted patents owned by London Biotechnology Ltd, and we can arrange non-exclusive licences for you. Make use of our experience to adapt this technology to your particular requirements: we work alongside your people in your labs, or we arrange contract research. This means that you get a much-improved product, without high devel-opment costs. A slide-show style presentation of this amplification system is on our site at: http://www.experts.co.uk/present.htm Stuart Harbron, PhD Experts http://www.experts.co.uk Tel: +44 (0) 1442 873012 Fax: +44 (0) 1442 384084 From jlm01 at mailclub-internet.fr Tue Jun 24 13:44:21 1997 From: jlm01 at mailclub-internet.fr (Martin) Date: Mon Mar 7 07:30:07 2005 Subject: Immunodeficit caused by lymphopenia t3 t4 (hiv - ) Message-ID: <01bc80ce$4a8e7680$d6c775c2@clubinternet.club-internet.fr> Immunodeficit caused by lymphopenia T3 T4 (HIV -) Summary : great CD 8 deficit and impossibility to stimulate these cells moderate CD 4 deficit there is not doubt that these anomalies are the cause or predisposed to HPV disease (important PS : HIV -) Study of a clinical case Female ,45 years old Medical history : 1. from her childhood epidermodysplasia verruca*, HPV8 bound *epidermis dyspasia like warts 2. 30 years old : ocular toxoplasmosis 3. 36 years old : breast tumor, treated with surgery, radiotherapy, chimiotherapy (6 treatments with Velbé + methotrexate + fluorouracile + cortisone) 4. 44 years old : cervix uteri biopsy : HPV related to 31.35.39 (probably HPV 51) vagina biopsy : HPV related to 31.35.39 and HPV related to 6.11.42 vulva biopsy : HPV related to 6.11.42 Conclusions of Immological studies T Lymphocytes Results Mean number % /mm3 /mm3 Lymphocytes total 7 500 1000-4000 CD 3+ 800-2500 CD 2+ 800-2500 CD 4+ 56.5 280 500-2000 CD 8+ 14.5 70 250-1000 Ratio CD 4+/CD 8+ 3.9 1.5-3 Lymphocyte phenotyping confirms great T lymphopenia (76.5 % CD 3 + cells, say 310mm3); there is CD4 lymphopenia worthy of note : 62 %, say 250 CD 3+ + CD 4+ cells/mm3 ; CD 8+ lymphopenia is great : 13 % CD 3+ + CD 8+ cells, say 59/mm3. We found again a decrease of CD45RA cells among CD 3+ ones : 5.5 % (whereas 92% of CD 3 + cells are CD45RA). Expression deficit of CD45RA is probably for its greater part on CD 4+ cells, since 30% of CD 8+ cells express CD45RA (we were not able to determine these labels on CD 4, because we did not possess the anti-CD 4 antibody marked with proper fluorochrome. Relating to activating labels, we found 18 % of CD 3+ cells with CD 25 and 20% of CD4 ones ; in the contrary CD25 expressing is very low on CD 8 + cells 1 %. CD 69 molecule is not a constituent element of T cells (CD 4 or CD 8). We purified mononucleated cells of Mrs X and cultivated them, during 18 hours without and with various otimuli. There is spontaneously for cells cultivated without stimulus, cellular activation as 63.5 % of CD 4+ cells express CD 69 molecule after 18 hours (compared to 20 % for normal cells), when stimulated with PHA 90 % of CD 4 cells but 34 % of CD 8 express CD 69 (compared to 80-98 % on CD 8 normal cells). For cells stimulated with anti-CD 3, CD 59 expressing is difficult to interpret because soluble CD 3 was more effective than fixed one. Conclusion : There is greater CD 8 lymphopenia, prevalence of memory cells through CD 4 cells seems, this cells spontaneously activated. On the contrary, there is probably insufficiency of CD 8+ cells activation. The remainder of the study have not reveal specific anomaly. The amount of gammaglobulins is normal : serum Ig G = 10 g/l ; normal Ig A, Ig M amounts ; normal serum complement. There is no autoantibody (anti-nuclear, anti DNA, anti-cardiolipin, rumatholoid factor) ; the intradermoreaction (10 units) is negative but Mrs X was not BCG-vaccinated ; blood count and formula are normal except lymphopenia. There is not specific anormaly of myelogram from spinal cord biopsy. I have no explanation for this immunological deficit but its features are compatible with specific decrease of anti-virus and anti-tumor defences. Do you know a treatment for increasing the immunity of this patient ? Do you know similar cases ? Where to obtain further information ? Answer to mee. jlm01@club-internet.fr Best tanks. From ethan at unixg.ubc.ca Tue Jun 24 17:28:12 1997 From: ethan at unixg.ubc.ca (Dr. Neil Reiner) Date: Mon Mar 7 07:30:07 2005 Subject: PDF position University of British Columbia Message-ID: <33B049FC.C9E@unixg.ubc.ca> PDF position, microbial pathogenesis, University of British Columbia A position is available to study gene expression in macrophages infected with M. tuberculosis and other mycobacteria. This position is in the Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, Canada. Contact Dr. Neil Reiner by email: ethan@unixg.ubc.ca From Ugom at fagmed.uit.no Tue Jun 24 04:46:27 1997 From: Ugom at fagmed.uit.no (Ugo Moens) Date: Mon Mar 7 07:30:07 2005 Subject: GFP immunization Message-ID: Hello, Has anybody tried to make his/her own antibodies against GFP or does anyone have experience with plasmid inocculation with GFP expression plasmids? Give such plasmid inocculation strong antigen-responses? We have inocculated Balb/c mice with pGFP-C1 (one single injection with 50 micrograms in each leg) plasmid DNA. This resulted in weak anti-GFP antibody response. I appreciate your response. Ugo Moens -- Ugo Moens University of Tromsø Institute of Medical Biology Dept. of Gene Biology N-9037 Tromsø Norway email: ugom@fagmed.uit.no From bryan at freja.fkem2.lth.se Tue Jun 24 03:03:32 1997 From: bryan at freja.fkem2.lth.se (Bryan Finn) Date: Mon Mar 7 07:30:07 2005 Subject: BIACORE data conversion Message-ID: <5onv0k$3pr$1@news.lth.se> Hi, We're looking for a small program or script that will convert the data from a Biacore into a format readable by other scientific plotting/speadsheet software (preferably ascii format). Does anyone have such a program for PC, Mac or UNIX? Thanks in advance. Bryan Finn ___________________________________________________________________ | | | Dr. Bryan Finn | | Department of Physical Chemistry 2 Tel: +46-46-222-8254 | | Chemical Center Fax: +46-46-222-4543 | | University of Lund | | POB 124 e-mail: bryan@bor.fkem2.lth.se | | S-221 00 Lund Sweden WWW: http://www.fkem2.lth.se/~bryan | |_________________________________________________________________| From Gerhard.nebe-von-caron at unilever.com Mon Jun 23 20:58:04 1997 From: Gerhard.nebe-von-caron at unilever.com (Gerhard Nebe-v.Caron) Date: Mon Mar 7 07:30:07 2005 Subject: Effects of fixatives on fluorescence ? Message-ID: <33AF9A3E.657F@unilever.com> Hi folks I wonder if somebody else has observed a reduction of phycoerythrine fluorescence upon paraformaldehyde fixation. In an attempt to measure antibody clusters in heparinised blood by flow cytometry, I used a distilled water lyse as commercial systems did not perform satisfactory. Unfortunately all PE markers tested so far (cd14, cd45ro, cd 4) show significantly higher fluorescence compared to cells treated with commercial lysing systems which leaves me with problems regarding compensation. So I will try to compare expression levels in gradient blood and in unlysed samples over the next few days, I thought somebody might have another idea. Thanks in advance for any input Gerhard From kristian at bioc.unizh.ch Tue Jun 24 12:27:33 1997 From: kristian at bioc.unizh.ch (Kristian Mueller) Date: Mon Mar 7 07:30:07 2005 Subject: BIACORE data conversion References: <5onv0k$3pr$1@news.lth.se> Message-ID: <33B00385.E75@bioc.unizh.ch> Hello Bryan Finn, why can?t you use the BIAcore software itself, which easily exports ASCII readible by spreadsheets like excel? In windows 3.1 there is a macro recorder allowing to automate some tasks. Regards Kristian Bryan Finn wrote: > > Hi, > We're looking for a small program or script that will convert the data from > a Biacore into a format readable by other scientific plotting/speadsheet software > (preferably ascii format). Does anyone have such a program for PC, Mac or UNIX? > > Thanks in advance. > > Bryan Finn > > ___________________________________________________________________ > | | > | Dr. Bryan Finn | > | Department of Physical Chemistry 2 Tel: +46-46-222-8254 | > | Chemical Center Fax: +46-46-222-4543 | > | University of Lund | > | POB 124 e-mail: bryan@bor.fkem2.lth.se | > | S-221 00 Lund Sweden WWW: http://www.fkem2.lth.se/~bryan | > |_________________________________________________________________| -- ___________________________ Kristian Mueller Dept. of Biochemistry phone: ++41-1-257-5588 University of Zurich fax: ++41-1-257-5712 Winterthurerstr. 190 e-mail: kristian@bioc.unizh.ch CH-8057 Zurich, Switzerland http://130.60.168.169/kristian From Jan.Michel at Klinik.Uni-Regensburg.de Tue Jun 24 20:42:08 1997 From: Jan.Michel at Klinik.Uni-Regensburg.de (Jan Michel) Date: Mon Mar 7 07:30:07 2005 Subject: antibody against housekeeping protein? Message-ID: <33B07770.7C35@Klinik.Uni-Regensburg.de> Hello folks, does anybody know where to buy an antibody against some sort of housekeeping protein such as G3PDH which can be used for western bloting? Thanks in advance! Jan From bioinquiry at aol.com Tue Jun 24 11:38:16 1997 From: bioinquiry at aol.com (Bioinquiry) Date: Mon Mar 7 07:30:07 2005 Subject: Scholarships for Cell Culture Workshop Message-ID: <19970624163800.MAA15491@ladder01.news.aol.com> Scholarships for Cell Culture Workshop Several corporate sponsored academic scholarships are available for two Cell Culture Workshops to be held at the Biotechnology Training Institute, Bridgewater, New Jersey. The workshops, scheduled for July 22-25 and October 14-17, will cover aseptic technique, establishment of primary cell cultures, manipulation of cultured cells, cell cloning, validation and cell line maintenance, growth optimization, transfection techniques, cell staining, and protein/DNA/RNA harvesting methods. The Cell Culture Workshop schedule can be obtained via email at "bioinquiry@aol.com" or by contacting the Institute at 908-253-3444. Several academic scholarships have been established for faculty, fellows, post-doctorates, and graduate students. Each scholarship covers cost of tuition and room. Travel expenses may be reimbursed at the discretion of the corporate sponsor. Interested individuals need to apply for a scholarship by submitting a current CV and a one page letter which briefly explains how cell culture will have a positive impact on their research. The CV and letter should be FAXed to 908-575-1660 or mailed to: Biotechnology Training Institute Cell Culture Scholarships 672 Route 202-206 North Bridgewater, NJ 08807 For additional information, please contact the Biotechnology Training Institute at 908-253-3444. From mkc125 at psu.edu Tue Jun 24 10:29:50 1997 From: mkc125 at psu.edu (Melissa Callahan) Date: Mon Mar 7 07:30:07 2005 Subject: blocking Fc receptors Message-ID: I'm trying to detect CD14 on THP-1 cells (human moncyte line) but am having some trouble. I've been using human IgG to block the Fc receptors then following with anti-CD14 and then FITC conjugated secondary Ab. Is there any reason why this should not work? I'm kinda at a loss here so any help would be greatly appreciated. Thanks in advance-- Missy From stavrosz at med.auth.gr Tue Jun 24 23:22:31 1997 From: stavrosz at med.auth.gr (Stavros P. Zanos) Date: Mon Mar 7 07:30:07 2005 Subject: References in Neuro-immune-endocrinology (List of replies). Message-ID: <5oq6em$2oe@evia.ccf.auth.gr> Some days ago I placed a query on a number of newsgroups, including this one, about some introductory or advanced references in the field of neuro-immune-endocrinology, being especially interested in the effects of cytokines on the hypothalamus-pituitary-gonadal glands axis. I received some replies, which I used to compile the following list: A. Journals of interest: Progress in Neuroendocrinimmunology (Thieme Medical Publishers) Journal of Neuroimmunology (Elsevier Science) Advances in Neuroimmunology (Elsevier Science) Brain, Behavior, and Immunity (Academic Press) Journal of Neuroendocrinology (Blackwell Science, Editor: Leng) Frontiers in Neuroendocrinology (Academic Press) B. Articles of interest: del Rey A, Furukawa H, Monge-Arditi G, Kabiersch A, Voigt KH, Besedovsky HO. Alterations in the pituitary-adrenal axis of adult mice following neonatal exposure to interleukin-1. Brain Behav Immun 1996, 10:3 235-48. Besedovsky HO, del Rey A. Immune-neuro-endocrine interactions: facts and hypotheses. Endocr Rev 1996, 17:1 64-102. Del Rey A, Besedovsky HO. Metabolic and neuroendocrine effects of pro-inflammatory cytokines. Eur J Clin Invest 1992, 22 Suppl 1: 10-5. Besedovsky HO, del Rey A. Immune-neuroendocrine circuits: integrative role of cytokines. Front Neuroendocrinol 1992 13:1 61-94. Besedovsky HO, del Rey A, Klusman I, Furukawa H, Monge Arditi G, Kabiersch A. Cytokines as modulators of the hypothalamus-pituitary-adrenal axis. J Steroid Biochem Mol Biol 1991 40:4-6 613-8. Besedovsky HO, del Rey A. Feed-back interactions between immunological cells and the hypothalamus-pituitary-adrenal axis. Neth J Med 1991 Oct 39:3-4 274-80. Berkenbosch F, de Rijk R, Del Rey A, Besedovsky H. Neuroendocrinology of interleukin-1. Adv Exp Med Biol 1990, 274: 303-14. Thomas DN; Post RM; Pert A. Central and systemic corticosterone differentially affect dopamine and norepinephrine in the frontal cortex of the awake freely moving rat. Ann N Y Acad Sci, 1994 746: 467-9 Brines R. Neuroendocrineimmunology today. Immunol Today. 1994; 15(11):503. Dardenne M & Savino W. Control of thymus physiology by peptidic hormones and neuropeptides. Immunol Today. 1994; 15(11): 518-23. Wilder RL. Neuroendocrine-immune system interactions and autoimmunity. Annu Rev Immunol. 1995; 13:307-38. Immune-neuroendocrine interactions. Immunol Today. 1995; 16(7):318-22 Science. 1997; 275; 1897-8 Endocrine Rev. 1996; 17: 64-102 Black, P. H. Immune system-central nervous system interactions: Effect and immunomodulatory consequences of immune system mediators on the brain. antimicrobial Agents and Chemotherapy 1994; 38(1): 7-12 Blalock, J.E. A molecular basis for bi-directional communication between the immune and neuroendocrine systems. Physiological Reviews 1989; 69(1):1-31 Blalock, J.E. The syntax of immune-neuroendocrine communication. Immunology Today 1994; 15(11):504-511 B. Special thanks to (in order of reply): Pier Carlo Montecucchi < pcmontecucchi@compuserve.com>, Siham Salmen Halabi , Josh Backon , Carolyn Field , Angelina Lo . From umnarj at mozart.inet.co.th Wed Jun 25 13:20:34 1997 From: umnarj at mozart.inet.co.th (Umnarj Paeratakul) Date: Mon Mar 7 07:30:07 2005 Subject: "Aquacide" Protein Concentrator Message-ID: <33B16172.55FD@mozart.inet.co.th> Dear friends, Long time ago, I concentrate protein in dilute solution by putting it in a dialysis bag (with very small holes), sprinkle AQUACIDE over the bag, and leave it overnight at 4 degrees. Next morning, aquacide will suck up all the water from the dialysis bag and leave the concentrated protein in there. My problem is now I cannot remember what company make AQUACIDE, or if you know a similar product, I would appreciate your help. Please reply by e-mail umnarj@mozart.inet.co.th Thank you in advance Umnarj From MEDKGM at leeds.ac.uk Wed Jun 25 09:50:02 1997 From: MEDKGM at leeds.ac.uk (MEDKGM@leeds.ac.uk) Date: Mon Mar 7 07:30:08 2005 Subject: EAhy.926 how do I get some? Message-ID: <33B19FD2.634@leeds.ac.uk> I am trying to get hold of EAhy.926, a transformed HUVEC cell line but none of the papers I've traced so far give a supplier. I would be grateful if anyone could e-mail me with any info Kevin Mercer K.G.Mercer@leeds.ac.uk From fredhyde at citilink.com Wed Jun 25 11:28:33 1997 From: fredhyde at citilink.com (Frederick W. Hyde) Date: Mon Mar 7 07:30:08 2005 Subject: Anti-alpha-fetoprotein MAb available Message-ID: <33B14731.57A8@citilink.com> Hello, I'm not sure if this is where this message can be posted but... I've just completed a project and have a substantial amount of monoclonal antibody to human alpha-fetoprotein (Bob Vessella clone AFP-27) in tissue culture available for sale. The antibody is stored frozen at -80C and is at a concentration of 1.1 mg/ml in McCoy's 5A medium with 5% FBS. I have perhaps 1-1.5 grams of the antibody available. If you are interested, please E-mail me at fredhyde@citilink.com. Thanks in advance, Fred From sef at med.unc.edu Wed Jun 25 04:27:01 1997 From: sef at med.unc.edu (Stacy Ferguson) Date: Mon Mar 7 07:30:08 2005 Subject: "Aquacide" Protein Concentrator References: <33B16172.55FD@mozart.inet.co.th> Message-ID: <5oqo95$qsm$1@fddinewz.oit.unc.edu> In article <33B16172.55FD@mozart.inet.co.th>, Umnarj Paeratakul wrote: >Dear friends, > >Long time ago, I concentrate protein in dilute solution by >putting it in a dialysis bag (with very small holes), >sprinkle AQUACIDE over the bag, and leave it overnight >at 4 degrees. Next morning, aquacide >will suck up all the water from the dialysis bag and >leave the concentrated protein in there. > >My problem is now I cannot remember what company make >AQUACIDE, or if you know a similar product, I >would appreciate your help. Please reply by >e-mail umnarj@mozart.inet.co.th I've used it in the past and if I recall correctly, it was sold by Fluka. However, it's not necessary to look that hard for it because it's just a cheap grade of polyethylene glycol (as opposed to the small batch nucleic acid grade). Most labs have the stuff lying around so you're probably set. I still use it because it's cheaper than Centricon or similar systems and more importantly, I tend to lose less protein to the dialysis tubing/PEG procedure. Stacy From stavrosz at med.auth.gr Tue Jun 24 23:22:31 1997 From: stavrosz at med.auth.gr (Stavros P. Zanos) Date: Mon Mar 7 07:30:08 2005 Subject: References in Neuro-immune-endocrinology (List of replies). Message-ID: <5oqb7d$8pc@evia.ccf.auth.gr> Some days ago I placed a query on a number of newsgroups, including this one, about some introductory or advanced references in the field of neuro-immune-endocrinology, being especially interested in the effects of cytokines on the hypothalamus-pituitary-gonadal glands axis. I received some replies, which I used to compile the following list: A. Journals of interest: Progress in Neuroendocrinimmunology (Thieme Medical Publishers) Journal of Neuroimmunology (Elsevier Science) Advances in Neuroimmunology (Elsevier Science) Brain, Behavior, and Immunity (Academic Press) Journal of Neuroendocrinology (Blackwell Science, Editor: Leng) Frontiers in Neuroendocrinology (Academic Press) B. Articles of interest: del Rey A, Furukawa H, Monge-Arditi G, Kabiersch A, Voigt KH, Besedovsky HO. Alterations in the pituitary-adrenal axis of adult mice following neonatal exposure to interleukin-1. Brain Behav Immun 1996, 10:3 235-48. Besedovsky HO, del Rey A. Immune-neuro-endocrine interactions: facts and hypotheses. Endocr Rev 1996, 17:1 64-102. Del Rey A, Besedovsky HO. Metabolic and neuroendocrine effects of pro-inflammatory cytokines. Eur J Clin Invest 1992, 22 Suppl 1: 10-5. Besedovsky HO, del Rey A. Immune-neuroendocrine circuits: integrative role of cytokines. Front Neuroendocrinol 1992 13:1 61-94. Besedovsky HO, del Rey A, Klusman I, Furukawa H, Monge Arditi G, Kabiersch A. Cytokines as modulators of the hypothalamus-pituitary-adrenal axis. J Steroid Biochem Mol Biol 1991 40:4-6 613-8. Besedovsky HO, del Rey A. Feed-back interactions between immunological cells and the hypothalamus-pituitary-adrenal axis. Neth J Med 1991 Oct 39:3-4 274-80. Berkenbosch F, de Rijk R, Del Rey A, Besedovsky H. Neuroendocrinology of interleukin-1. Adv Exp Med Biol 1990, 274: 303-14. Thomas DN; Post RM; Pert A. Central and systemic corticosterone differentially affect dopamine and norepinephrine in the frontal cortex of the awake freely moving rat. Ann N Y Acad Sci, 1994 746: 467-9 Brines R. Neuroendocrineimmunology today. Immunol Today. 1994; 15(11):503. Dardenne M & Savino W. Control of thymus physiology by peptidic hormones and neuropeptides. Immunol Today. 1994; 15(11): 518-23. Wilder RL. Neuroendocrine-immune system interactions and autoimmunity. Annu Rev Immunol. 1995; 13:307-38. Immune-neuroendocrine interactions. Immunol Today. 1995; 16(7):318-22 Science. 1997; 275; 1897-8 Endocrine Rev. 1996; 17: 64-102 Black, P. H. Immune system-central nervous system interactions: Effect and immunomodulatory consequences of immune system mediators on the brain. antimicrobial Agents and Chemotherapy 1994; 38(1): 7-12 Blalock, J.E. A molecular basis for bi-directional communication between the immune and neuroendocrine systems. Physiological Reviews 1989; 69(1):1-31 Blalock, J.E. The syntax of immune-neuroendocrine communication. Immunology Today 1994; 15(11):504-511 B. Special thanks to (in order of reply): Pier Carlo Montecucchi < pcmontecucchi@compuserve.com>, Siham Salmen Halabi , Josh Backon , Carolyn Field , Angelina Lo . From gheavner at voicenet.com Wed Jun 25 18:46:47 1997 From: gheavner at voicenet.com (George A. Heavner) Date: Mon Mar 7 07:30:08 2005 Subject: BIACORE data conversion References: <5onv0k$3pr$1@news.lth.se> Message-ID: <33B1ADE7.6640@voicenet.com> From eric-rumsey at uiowa.edu Wed Jun 25 13:39:42 1997 From: eric-rumsey at uiowa.edu (Eric Rumsey) Date: Mon Mar 7 07:30:08 2005 Subject: WWW: Hardin Meta Directory update - Allergy/Immunology Message-ID: This is to announce an updated version of the Hardin Meta Directory web page for Allergy/Immunology. All links have been checked to confirm connection and new links have been added, including the following: -Antibody Resource Page, Kevin Shreder, Univ Calif/San Diego -Cliniweb: Immunologic Diseases, Oregon Health Sciences Univ A *new and important feature* for Hardin MD is that we are now using a link checker to check the connection rate for the lists that are included. Generally the lists with better connection rates are toward the top of their size category (although size of the list is also a consideration in placement). Lists on the updated Hardin MD Allergy/Immunology page with especially good connection rates include: -MedWeb: Immunology -MedMark - Immunology, I Shin, MedMark Team, Korea -Antibody Resource Page, Kevin Shreder, Univ Calif/San Diego -Allergy Internet Resources, Ballew Kinnaman, Allergy Mailing List -Latex Allergy Links, NA Mitchell -Karolinska MIC-KIBIC MeSH Index - Immunologic Diseases -Medical Matrix - Allergy -Medical Matrix - Immunology -Cliniweb: Immunologic Diseases, Oregon Health Sciences Univ -Asthma and Allergy WWW Resources Page, Patricia Wrean. Of course, using our link checker, the links on our own pages have superlative connection rates, generally above 98%. The URL for the Hardin Meta Directory Allergy/Immunology page is - http://www.arcade.uiowa.edu/hardin-www/md-allergy.html Please check it out ! Please post this message to appropriate listservs. If you would like to receive e-mail notices for all Hardin MD updates (1-2 messages per wk), please e-mail me directly at rumsey@blue.weeg.uiowa.edu. DO NOT REPLY DIRECTLY TO THIS MESSAGE. * * * * * * * * * Eric Rumsey, Hardin Library for the Health Sciences University of Iowa, Iowa City IA 52242 319-335-9875 (voice), 319-335-9897 (fax) Hardin Meta Directory of Internet Health Sources http://www.arcade.uiowa.edu/hardin-www/md.html Reviewed in Consumer Reports, Feb 1997, p 29 From dana32 at aol.com Wed Jun 25 20:35:36 1997 From: dana32 at aol.com (Dana32) Date: Mon Mar 7 07:30:08 2005 Subject: CTLL-2 assay help Message-ID: <19970626013500.VAA17945@ladder02.news.aol.com> Hello ! Can somebody out there provide me a method of calculation for the CTLL-2 assay ? My e-mail address is Dana32@aol.com Thank you ! Dana From gkremmid at mad.adelaide.edu.au Thu Jun 26 02:33:10 1997 From: gkremmid at mad.adelaide.edu.au (Gabriel Kremmidiotis) Date: Mon Mar 7 07:30:08 2005 Subject: (none) Message-ID: <199706260733.RAA09171@pulse.health.adelaide.edu.au> unsubscribe From emiliano.mugnaini at labmed.uio.no Fri Jun 27 05:19:45 1997 From: emiliano.mugnaini at labmed.uio.no (Emiliano Mugnaini) Date: Mon Mar 7 07:30:08 2005 Subject: Conjugated Cytokines Wanted Message-ID: <33B393BF.3272@labmed.uio.no> Hello, I am interested in obtaining the cytokines listed below in conjugated (FITC or PE) or biotinylated form: IFN - alpha/beta TNF - alpha IL - 2,4,7,10,12. Is there anyone who might know where such can be found? I have already begun to inquire with R&D Systems, Genzyme, and Bender Medsystems. If necessary, I would be interested in having the cytokines custom conjugated or delivered in carrier-free form so that I could biotinylate them myself. Thank you, Emil Mugnaini From emiliano.mugnaini at labmed.uio.no Fri Jun 27 03:37:22 1997 From: emiliano.mugnaini at labmed.uio.no (Emiliano Mugnaini) Date: Mon Mar 7 07:30:08 2005 Subject: Conjugated Cytokines Wanted Message-ID: <33B37BC0.492E@labmed.uio.no> Hello, I would like to purchase the cytokines listed below already conjugated to either FITC or PE, or biotinylated: IFN - alpha/beta, gamma. TNF - alpha. IL - 2,4,7,10,12. Is there anyone who might know where I could get a hold of such things? I have started already to inquire with R & D Systems, Genzyme, and Bender medsystems. I would be interested ordering a custom conjugation or having them in a carrier-free form so that I could biotinylate them myself, if that were necessary. Thank you, Emil Mugnaini From mdoherty at pop.niaid.nih.gov Thu Jun 26 14:13:14 1997 From: mdoherty at pop.niaid.nih.gov (Mark Doherty) Date: Mon Mar 7 07:30:08 2005 Subject: CTLL-2 assay help References: <19970626013500.VAA17945@ladder02.news.aol.com> Message-ID: In article <19970626013500.VAA17945@ladder02.news.aol.com>, dana32@aol.com (Dana32) wrote: > Hello ! Can somebody out there provide me a method of calculation for the > CTLL-2 assay ? > My e-mail address is Dana32@aol.com > Thank you ! > It's pretty straightforward. You need a standard - which is a growth factor the CTLL-2 will respond to (normally either IL-2 or IL-15) at a known concentration, which you can titrate onto your CTLL-2 cells at the same time as you put your samples on. How much of this standard you use is going to varydepending on how much activity tere is in your samples, but generally a good point to start your standard titration is about 10 u/ml, and titrate down two fold from there. Once you have a standard that overlaps the factor you have in your samples, it's easy to calculate the activity ofthe samples. If 1 u/ml of your IL-2 standard gives you 60,000 cpm and your sample gives you (say) 80,000 cpm then you have 1.33 u/ml in your sample. If your sample gives you 32,000 then you have 0.53 u/ml and so on. Of course to attribute this activity to your growth factor of interest, you should show that you can block it with saturating levels of the appropriate monoclonal antibody. Cheers, Mark From rjjensen at inav.net Fri Jun 27 18:34:35 1997 From: rjjensen at inav.net (Robert J Jensen) Date: Mon Mar 7 07:30:08 2005 Subject: "Aquacide" Protein Concentrator References: <33B16172.55FD@mozart.inet.co.th> <5oqo95$qsm$1@fddinewz.oit.unc.edu> Message-ID: <33B44E0B.4701@inav.net> Aquacide is sold by Calbiochem. From malik at uranus.ebi.ac.uk Fri Jun 27 10:30:28 1997 From: malik at uranus.ebi.ac.uk (Ansar Malik) Date: Mon Mar 7 07:30:09 2005 Subject: ANNOUNCEMENT: Immunogenetics IMGT database - new release Message-ID: IMMUNOGENETICS IMGT DATABASE =================================== Professor Marie-Paule LEFRANC Laboratoire d'ImmunoGenetique Moleculaire, LIGM UMR CNRS 9942, Institut de Genetique Moleculaire BP 5051, 1919 route de Mende, 34033 MONTPELLIER Cedex 1 FRANCE Telephone: (33) 67 61 36 34 FAX : (33) 67 04 02 31/67 04 02 45 e-mail : lefranc@ligm.crbm.cnrs-mop.fr INTRODUCTION ============ The ImMunoGeneTics database, IMGT, is an integrated specialised database containing nucleotide sequence information of genes important in the function of the immune system. It collects and annotates sequences belonging to the immunoglobulin superfamily which are involved in immune recognition, these are the B cell antigen receptor (Immunoglobulin or Ig), the T cell antigen receptor (TCR) (LIGM-database) and the class I and class II molecules of the Human Leucocyte Antigens (HLA) system (IMGT/HLA-database under development). IMGT/LIGM DATABASE ================== An integrated immumogenetics database (IMGT/LIGM) specialising in Ig and TcR is under development through collaboration between LIGM, IFG and EMBL oustation EBI. This database consists of the Ig and TcR sequence entries. Collaborators: Release 97.06 contains 8622 fully annotated sequence entries. LIGM Montpellier :Marie-Paule Lefranc (coordinator) Veronique Giudicelli,Denys Chaume EMBL-EBI :Ansar Malik IFG :Werner Mueller ACCESS/DATA DISTRIBUTION ======================== SRS server: http://srs.ebi.ac.uk:5000/ NOTE: Selecting this option will bring you to the SRS qerry interface page, where you have to select "search Sequence Libraries" WWW server: http://www.ebi.ac.uk/imgt/ FTP server: ftp.ebi.ac.uk/pub/databases/imgt E_MAIL server: email netserv@ebi.ac.uk -- send "help IMGT" in the mail body. This database is available on CD-ROM as an acompanying database to the EMBL Nucleotide Sequence Database at nominal cost. ======================================================================= Ansar Malik Ph.D | Email:Malik@ebi.ac.uk EBI - European Bioinformatics Institute | URL: http://www.ebi.ac.uk Hinxton Hall, Hinxton | Tel: +44 (1223) 494417 Cambridge CB10 1RQ, UK | Fax: +44 (1223) 494968 ======================================================================== From smori at NMSU.Edu Fri Jun 27 21:22:12 1997 From: smori at NMSU.Edu (S. MORI) Date: Mon Mar 7 07:30:09 2005 Subject: NK/Macrophage interactionm Message-ID: <5p1sgk$k7n@bubba.NMSU.Edu> Dear Immunologists... I have been reading up on a drug currently in clinicical trials (maxamine) which apparently prevents the intersaction between Macrophages and NK cells...As it turns out, this interaction results in apoptosis of NK cells and therefore, lower efficiency of tumour killing by NK cells/// Has anyone heard of a paper that describes such an interaction??? I would love to follow up on this NK/Macro contact.... I would like to hear your comment on this... Thanks.. -- _/\_ _\ /_ \_ _/ || C A N A D A DNA$DNA$DNA$DNA$DNA$DNA$DNA$DNA$DNA$DNA$DNA$DNA$DNA$DNA$DNA$DNA Shahram Mori Program in Molecular Biology Department of Chemistry and Biochemistry Box 3C New Mexico State University Las Cruces NM 88003 RNA$RNA$RNA$RNA$RNA$RNA$RNA$RNA$RNA$RNA$RNA$RNA$RNA$RNA$RNA$RNA From mlj at sanasys.com Sun Jun 29 16:44:29 1997 From: mlj at sanasys.com (Maurice Jensen) Date: Mon Mar 7 07:30:09 2005 Subject: blocking Fc receptors References: Message-ID: <33B6D73D.310D@sanasys.com> Melissa Callahan wrote: > > I'm trying to detect CD14 on THP-1 cells (human moncyte line) but am having > some trouble. I've been using human IgG to block the Fc receptors then > following with anti-CD14 and then FITC conjugated secondary Ab. Is there any > reason why this should not work? I'm kinda at a loss here so any help would > be greatly appreciated. > Thanks in advance-- Missy I hope your Primary is not unconjugated followed by Anti Human-Fitc or it will not work as I described in my previous response. From frijters at concepts.nl Sun Jun 29 05:40:56 1997 From: frijters at concepts.nl (Raoul Frijters) Date: Mon Mar 7 07:30:09 2005 Subject: IL-4 Message-ID: <01bc8478$fb5c3f80$d80786c2@default> Hi, Does someone knows a site where I can find information about IL-4? (structure, function, etc.) R.Frijters From mlj at sanasys.com Sun Jun 29 16:41:04 1997 From: mlj at sanasys.com (Maurice Jensen) Date: Mon Mar 7 07:30:09 2005 Subject: blocking Fc receptors References: Message-ID: <33B6D670.3769@sanasys.com> Melissa Callahan wrote: > > I'm trying to detect CD14 on THP-1 cells (human moncyte line) but am having > some trouble. I've been using human IgG to block the Fc receptors then > following with anti-CD14 and then FITC conjugated secondary Ab. Is there any > reason why this should not work? I'm kinda at a loss here so any help would > be greatly appreciated. > Thanks in advance-- Missy Try using whole Human serum instead of just IgG. Pre-incubate your cells with 50% Human Serum for 10 min. at 4C then keep at least 5% Human serum in your staining solutions. Good Luck From john at rm101.demon.co.uk Mon Jun 30 13:39:59 1997 From: john at rm101.demon.co.uk (John C) Date: Mon Mar 7 07:30:09 2005 Subject: 8 well neutrophil slides- how to make them? Message-ID: <33b7fcc3.6307490@news.demon.co.uk> Hi all I need a good method for making 8 well neutrophill slides for ANCA testing, we have tried some methods but the quality is a bit poor. Cytospin methods are ok but are limited to single well slides(At least with our machine!). If any body has a good method or can point me in the right direction I would be eternally grateful. Many Thanks John From holeung at ucla.edu Fri Jun 27 20:21:44 1997 From: holeung at ucla.edu (Ng) Date: Mon Mar 7 07:30:09 2005 Subject: homology between antigen and MHC? Message-ID: <5p1ov8$24u6@uni.library.ucla.edu> Hello. In Stryer's Biochemistry textbook, he writes that MHC molecules contain the consensus sequence of the antigen peptides that they bind, "thus, peptides capable of being presented by an MHC protein are homologus to a portion of the MHC protein itself." I have been unable to find papers that mention this homology. No mention is made of this in the recent reviews I've looked through. Any leads? Thank you! -- Ho Leung Ng holeung@ucla.edu From K_Vora at lac.jci.tju.edu Mon Jun 30 16:18:40 1997 From: K_Vora at lac.jci.tju.edu (Kalpit A. Vora) Date: Mon Mar 7 07:30:09 2005 Subject: Immunostaining of mouse spleen with rabbit antisera Message-ID: Dear Readers, I am trying to stain mouse splenic cryosection with an polyclonal antisera raised in rabbits. I am having a terrible background staining problem. I do block with buffer containing 5% normal mouse serum(NMS) and 5% normal goat serum(NGS). Also all dilution of my primary and secondary antibodies are done in the above buffer containing NMS and NGS. Any pointers to improve my staining and decrease my background will be very helpful and thankyou in advance for the same. My e-mail address is as follows: kvora@lac.jci.tju.edu. Kalpit A Vora From frauwirt at bacillus.Berkeley.EDU Mon Jun 30 14:37:03 1997 From: frauwirt at bacillus.Berkeley.EDU (Ken Frauwirth BioKen) Date: Mon Mar 7 07:30:09 2005 Subject: homology between antigen and MHC? References: <5p1ov8$24u6@uni.library.ucla.edu> Message-ID: <5p91sv$1a0@agate.berkeley.edu> In article <5p1ov8$24u6@uni.library.ucla.edu>, Ng wrote: > Hello. In Stryer's Biochemistry textbook, he writes that MHC >molecules contain the consensus sequence of the antigen peptides that >they bind, "thus, peptides capable of being presented by an MHC protein >are homologus to a portion of the MHC protein itself." I have been >unable to find papers that mention this homology. No mention is made >of this in the recent reviews I've looked through. > Any leads? Thank you! I think Stryer may be confused on this one. I am currently doing my grad research on MHC Class II antigen processing, and I have never heard of this. While it *is* true that peptides from MHC molecules can be presented by other MHC molecules, I don't know of any study showing a homology between an MHC molecule and the peptides it is able to present. For what it is worth, the motifs for MHC binding are not very complex - usually just 2-3 amino acids in specific spacing arrangements - so there is a good chance that a protein as big as an MHC molecule is going to have peptides that can bind to most MHC molecules, including itself. Ken Frauwirth -- Ken Frauwirth (MiSTie #33025) _ _ frauwirt@mendel.berkeley.edu |_) * |/ (_ |\ | http://www.ocf.berkeley.edu/~frauwirt/ |_) | () |\ (_ | \| DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs From ez052183 at mailbox.ucdavis.edu Mon Jun 30 16:40:39 1997 From: ez052183 at mailbox.ucdavis.edu (David Verhoeven) Date: Mon Mar 7 07:30:09 2005 Subject: Lymphokine Elisa Message-ID: Does anyone know of a good protocol for detecting active lymphokines within serum samples? Thank you for you help, David Verhoeven From fd601220 at ntu.ac.uk Mon Jun 30 19:07:06 1997 From: fd601220 at ntu.ac.uk (fd601220@ntu.ac.uk) Date: Mon Mar 7 07:30:09 2005 Subject: Danger vs. self/non-self model Message-ID: <33B84A2A.5CC8@ntu.ac.uk> RecentHorizon tv prog convinces me that the self/non-self model is wrong; how will the fact that the 'danger' model isv.prob. the case change immunological treatments + reserch.How duz the danger model explain food allergies+ rejection of foreign grafts?