From arin280 from gmail.com Thu Apr 9 21:20:42 2009 From: arin280 from gmail.com (Arin280) Date: Fri Apr 10 10:13:44 2009 Subject: [Immunology] A Useful Website for Immunology Researchers - MyNetResearch.com Message-ID: Dear Immunology Researcher, With a team of academics, I recently launched the research website, MyNetResearch.com. Our website helps to make your research more productive. Specifically, you can: - manage your research papers and grant proposals online and access them from any location - conduct online surveys and citation searches - receive expert advice on research and statistical design - read about the latest research in hundreds of different subjects - exchange ideas with thousands of other researchers - collaborate with thousands of other researchers on your research papers Currently, over 6,000 researchers from 95 countries and multiple research specialties use MyNetResearch for their research. I=92m trying to spread the word about MyNetResearch and to let researchers know about this great resource. Regular membership is completely free. I invite you to try MyNetResearch. You can sign up at: https://www.mynetresearch.com/Signup/SignUp.aspx Sincerely, Bay Arinze, Ph.D., Founding Editor MyNetResearch, Empowering Collaboration=99 From hemendernamdev from trinitronbiotech.com Wed Apr 15 10:14:05 2009 From: hemendernamdev from trinitronbiotech.com (Hemender Kumar) Date: Wed Apr 15 12:01:40 2009 Subject: [Immunology] Plasma to serum protocols? Message-ID: Dear Sir, I need this protocol. Please help me. Thanks and best regards, Dr Hemender Kumar Manager QA/QC Trinitron Biotech Limited, Abuja, Nigeria From hemendernamdev from trinitronbiotech.com Wed Apr 15 10:14:05 2009 From: hemendernamdev from trinitronbiotech.com (Hemender Kumar) Date: Thu Apr 16 10:50:31 2009 Subject: [Immunology] Plasma to serum protocols? Message-ID: Dear Sir, I need this protocol. Please help me. Thanks and best regards, Dr Hemender Kumar Manager QA/QC Trinitron Biotech Limited, Abuja, Nigeria From mssudheesh from rediffmail.com Fri Apr 17 12:26:37 2009 From: mssudheesh from rediffmail.com (m s sudheesh) Date: Fri Apr 17 16:38:52 2009 Subject: [Immunology] ELISA protocol Message-ID: <20090417172637.64300.qmail@f4mail-235-241.rediffmail.com> Dear All, I am working on the development of nanoparticulate vaccine. While performing ELISA potocol the end point titre was expressed as the logarithm of the reciprocal of the last dilution which gave an optical density at 490nm above the optical density of negative control. What should be the negative control is it preimmune sera or the sera of animals trated with blank nanoparticles or the well containing only substrate. Kindly help me. M.S.Sudheesh,Research Fellow, Pharm Biotech Div, Department of Pharmaceutical Sciences, Dr. H.S.Gour University, Sagar, M.P,India,470001 Phone +91-9826415402 From carlos.duarte from cigb.edu.cu Fri Apr 17 16:50:17 2009 From: carlos.duarte from cigb.edu.cu (Carlos Duarte Cano) Date: Fri Apr 17 18:01:11 2009 Subject: [Immunology] ELISA protocol In-Reply-To: <20090417172637.64300.qmail@f4mail-235-241.rediffmail.com> Message-ID: <00925AA44B42854BB6C9BE98C589E86A012B6A3C@frodo.cigb.edu.cu> Dear Dr Sudheesh You may use both but the second one is better since some inespecific stimulation can slightly increase the background in the ELISA. The best is to have a group of sera and to use the mean and the STD to calculate the cut off. regards Carlos Duarte -----Original Message----- From: immuno-bounces@oat.bio.indiana.edu [mailto:immuno-bounces@oat.bio.indiana.edu] On Behalf Of m s sudheesh Sent: Friday, April 17, 2009 1:27 PM To: immuno@magpie.bio.indiana.edu Subject: [Immunology] ELISA protocol Dear All, I am working on the development of nanoparticulate vaccine. While performing ELISA potocol the end point titre was expressed as the logarithm of the reciprocal of the last dilution which gave an optical density at 490nm above the optical density of negative control. What should be the negative control is it preimmune sera or the sera of animals trated with blank nanoparticles or the well containing only substrate. Kindly help me. M.S.Sudheesh,Research Fellow, Pharm Biotech Div, Department of Pharmaceutical Sciences, Dr. H.S.Gour University, Sagar, M.P,India,470001 Phone +91-9826415402 _______________________________________________ Immuno mailing list Immuno@net.bio.net http://www.bio.net/biomail/listinfo/immuno From itabajara.vaz from ufrgs.br Mon Apr 20 17:12:05 2009 From: itabajara.vaz from ufrgs.br (Itabajara Vaz - UFRGS) Date: Mon Apr 20 19:19:13 2009 Subject: [Immunology] bacterias 41(DE3) e C43(DE3) Message-ID: <49ECF335.2080508@ufrgs.br> Hi, I am needing the C41(DE3), C41(DE3)pLysS and C43(DE3), C43(DE3)pLysS (E. coli bacterias) Does anybody have these bacterias? //Can anybody help me? Thank you very much, **C41(DE3), C41(DE3)pLysS and C43(DE3), C43(DE3)pLysS E. coli BL21(DE3) strains, like Lucigen's E. cloni EXPRESS Competent Cells provide reliable expression of many proteins cloned into T7 expression vectors (e.g., pET or Lucigen's pSMART?-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal. http://www.lucigen.com/catalog/index.php?cPath=47_58 sincerely -- Itabajara da Silva Vaz Junior Centro de Biotecnologia - UFRGS C.P. 15005 Av. Bento Goncalves 9500 Predio 43421 - Campus do Vale 91501-970 Porto Alegre RS Brasil Fone (51) 33086078 Fax (51)3308-73-09 [00 55 51 3308 7309] http://www.ufrgs.br/depbiot/201/cbt201.htm http://www.ufrgs.br/favet/imunovet From LiXin from uams.edu Tue Apr 21 11:24:50 2009 From: LiXin from uams.edu (Li, Xin) Date: Tue Apr 21 12:17:40 2009 Subject: [Immunology] Ceramide ELISA Message-ID: Dear Henk, I am very interested in the Ceramide ELISA. Did you develop the ELISA kit? Is it available in your lab or somewhere else? I appreciate your guide about it. Thank you! Regards, Xin Li, Ph.D. Myeloma Institute for Research and Therapy Winthrop P. Rockefeller Cancer Institute, Room 938 University of Arkansas for Medical Sciences 4301 W. Markham, Slot 776 Little Rock, Arkansas 72205 Tel: (501) 296-1503 ext. 1461 Fax: (501) 686-6442 E-mail: lixin@uams.edu Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From hssandeshrao from gmail.com Wed Apr 22 07:32:31 2009 From: hssandeshrao from gmail.com (sandesh rao) Date: Wed Apr 22 07:49:19 2009 Subject: [Immunology] indirect ELISA protocol Message-ID: Dear all, I am working on plant derived vaccines. I would like to know whether an indirect ELISA can be standardized to quantify the amount of antigen expressed in plants. Kindly let me know if any protocol is available. Sandesh Rao, Senior Research Fellow, Plant vaccine Lab, UAS, B'lore,India. - 560065. From jtaranjuez from gmail.com Wed Apr 22 10:28:36 2009 From: jtaranjuez from gmail.com (Justine Tigno-Aranjuez) Date: Wed Apr 22 11:37:41 2009 Subject: [Immunology] help with specific ICS protocol Message-ID: Hello All, I need to do some staining for mouse CD4, IFN-g, IL-17, and CCR6. I haven't tried this combination before and have difficulty doing ICS in general (loss of surface stains). Would anybody have any experience on a good clone and fluor combination for these markers. I am really grateful for any help. Thank you. Justine Tigno-Aranjuez