From ashis.rasaily from gmail.com  Mon Jul 13 00:51:11 2009
From: ashis.rasaily from gmail.com (ashis)
Date: Mon Jul 13 06:03:19 2009
Subject: [Immunology] hepatocytes and drug detoxification
Message-ID: <7b4fd8c8-23d2-4723-bbe5-ab0db70eb37e@v15g2000prn.googlegroups.com>

hi everybody,
                    any one working on hepatocyte culture and drug
detoxification? if yes we could discuss it thank you
 
ASHIS
From kittipongwarakarns from exchsvr.fwnc.net  Tue Jul 14 04:00:48 2009
From: kittipongwarakarns from exchsvr.fwnc.net (kittipongwarakarns)
Date: Tue Jul 14 08:12:04 2009
Subject: [Immunology] searching for data on extinction coefficients for
	equine seric Fab, F(ab')2
Message-ID: <0CA5AD19B687DA4EB16C6AF2B4B11C760C9EE0@FWNCEXCH2.fwnc.net>

Dear all,

 

I would like to know about extinction coefficients for equine seric Fab,
F(ab')2.

Did you find it before?

How did you get it?

 

Thank you in advance

 

Susan

From lloarca76 from yahoo.com  Tue Jul 14 14:55:47 2009
From: lloarca76 from yahoo.com (lloarca76@yahoo.com)
Date: Tue Jul 14 15:19:11 2009
Subject: [Immunology] neutrophil isolation protocol
Message-ID: <604835.83855.qm@web32405.mail.mud.yahoo.com>


Hello Bryan,
?
I read the protocol you posted online that describes how to isolate neutrophils from blood.? Is it possible to use this protocol to isolate neutrophils from liver biopsies of people with chronic liver diseases?
?
Actually, I need to isolate neutrophils, DCs and Kupffer cells and I haven't found a protocol that I can use for the separation of the three types of cells.? If you know a protocol that addresses this, I would greatly appreciate that information.
?
Thanks a lot,
?
Lorena Loarca
From vaxjobs from hotmail.com  Tue Jul 21 07:10:05 2009
From: vaxjobs from hotmail.com (Otto)
Date: Tue Jul 21 08:39:22 2009
Subject: [Immunology] cross-species single primary cell immunoglobulin
	production rates?
Message-ID: <1b1736c9-1368-4157-a161-8f09165fd27e@u38g2000pro.googlegroups.com>

I'm helping a modeler try to tackle a hairy vaccine dose prediction
problem, and need to scare up some data. Several hours of lit searches
have yielded some, but not enough information.

Once I got past all the hybridoma and tumor cell production rate
papers, I found several old papers on antibody production rates in
various primary cell systems.  Most of it was ex vivo, but little was
done to try to correlate that data with in vivo observation (either
tissue-specific rates or peripheral blood concentration). Also, there
is a broad range of estimates depending on the analysis done to make
those estimates (2000 molecules/cell/min to about seven times that).

Is anyone aware if there are direct comparisons done on rodent v.
primate Ab production rates?  In other words, is there a reason to
think that a maximally stimulated rodent plasma cell would produce
dramatically more or less IgM or IgG than a comparable human cell?
Thanks

v_a_x_j_o_b_s  @ h_o_t_m_a_i_l_d_o_t_c_o_m_