Fading problem with flourecein labelling

James B. Hutchins jbh at anat.UMSMED.EDU
Wed Dec 1 20:44:03 EST 1993


In article <2div26$6hv at netnews.upenn.edu> cychan at mail.sas.upenn.edu
(Cecilia Y Chan) writes:
>
>I am having a lot of problems with fading using flourescein label for Ab. 
>I have tried various mounting reagents, but they all seem to fade
[stuff deleted]

You do not say what you have tried, so excuse me if this is redundant...

We have had good luck with three formulations:

1) Commercial media such as FluorSave (Calbiochem) or whatever the name of
Molecular Probes' similar product is (Slow Fade, I think).  (I have no
connection to either of these companies.)

2) A product from Mallinckrodt diagnostics division called Conray (sold as
a contrast agent for X-rays, I think).

3) Homemade formulas containing n-propyl-gallate in buffered glycerol (I can
send references/recipes to anyone interested).

Have you tried any of these?  If so, I'm stumped!

Jim

-- 
Jim Hutchins                    []     E-Mail: jbh at anat.umsmed.edu
Asst Prof of Anatomy            []     Asst Prof of Neurology
Univ Mississippi Med Ctr        []     Jackson, MS



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