Fading problem with flourecein labelling
James B. Hutchins
jbh at anat.UMSMED.EDU
Wed Dec 1 20:44:03 EST 1993
In article <2div26$6hv at netnews.upenn.edu> cychan at mail.sas.upenn.edu
(Cecilia Y Chan) writes:
>I am having a lot of problems with fading using flourescein label for Ab.
>I have tried various mounting reagents, but they all seem to fade
You do not say what you have tried, so excuse me if this is redundant...
We have had good luck with three formulations:
1) Commercial media such as FluorSave (Calbiochem) or whatever the name of
Molecular Probes' similar product is (Slow Fade, I think). (I have no
connection to either of these companies.)
2) A product from Mallinckrodt diagnostics division called Conray (sold as
a contrast agent for X-rays, I think).
3) Homemade formulas containing n-propyl-gallate in buffered glycerol (I can
send references/recipes to anyone interested).
Have you tried any of these? If so, I'm stumped!
Jim Hutchins  E-Mail: jbh at anat.umsmed.edu
Asst Prof of Anatomy  Asst Prof of Neurology
Univ Mississippi Med Ctr  Jackson, MS
More information about the Immuno