Need method for isolating pure T from Human PBLs

Joe Garth Bloggs Suebag at bham.ac.uk
Tue Apr 11 09:50:28 EST 1995


In article <3mbk4v$239 at netnews.upenn.edu>, David Peritt <Peritt_d at a1.mscf.upenn.edu> says:
>
>In article <3m4okj$n03 at decaxp.harvard.edu> Derek V. Chan,
>chan4 at fas.harvard.edu writes:
>>Yep!  Unfortunately, panning only yields at best 97% clean....
>
>Getting better than 97% is going to be difficult.  Try using two
>procedures back to back.  But the loss will incresase dramatically and
>are you worried about the effect on the cells going through these
>procedures?

How about trying negative selection, using a cocktail of the following antibodies:
anti-CD16, CD19, CD 11c, HLA-DR, Glycophorin, CD56 (optional) and routinely
have yields >98% pure. This is following an adherent depletion stage.

Ta ta

Sue



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