> I am looking for any ideas on how to extract (not necessarily purify)
> either IgM or C4 (Complement 4) from raw human serum. We are exploring
> ways of elevating either protein in calibrator products and are looking
> for any helpful suggestions. Thanks.
> -Janene Lenard
> Protein Chemist
Janene, perhaps these citations will be useful.
Chemical Abstract Services Phone 614-447-3600 X3198
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TI Purification of human immunoglobulin M by affinity chromatography on
AU Wichman, Anders; Borg, Hakan
CS Res. Dep. Biochem., AB Kabi, Stockholm, Swed.
SO Biochim. Biophys. Acta (1977), 490(2), 363-9
TI Separation of different forms of the fourth component of human
complement by fast protein liquid chromatography
AU Hessing, Martin; Paardekooper, Jolanda; Hack, C. Erik
CS Cent. Lab. Neth. Red Cross Transfus. Serv., Amsterdam, Neth.
SO J. Immunol. Methods (1993), 157(1-2), 39-48