Is there anyone who have an experience of CTL assay with rat?

Seung woo Lee monk at postech.ac.kr
Sun Jun 8 21:50:47 EST 1997


Hi guys!

I had hard times to detect CTL response to specific viral antigen in
rat (Buffalo rat; RT1b). The major problem is the target cell. I used
the hepatoma cell line of BUF rat, but this cell has high percentage
of spontaneous Cr release and I wonder whether this cell has right
component such as MHC, accessory molecules etc. 
Is there anyone who have an experience of CTL assay with rat or give
me any idea to solving this problem?
 
Seung woo Lee
monk at postech.ac.kr



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