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Problem with ELISAs

Simon Easterbrook-Smith sbe at biochem.usyd.edu.au
Sun Nov 15 17:14:05 EST 1998

Dear Robin

How fresh is your H2O2?

We have sometimes seen similar symptoms to yours if the bottle is a bit elderly.


In article <72ht2h$53i$1 at nntp.Stanford.EDU>, robinb at leland.Stanford.EDU
(Robin Blumenthal) wrote:

|| We are having an interesting problem in our lab with our ELISAs. I was
|| wondering if anyone out there might have any ideas.
|| We are doing sandwich ELISAs for cytokines and Igs (both human and murine)
|| using a biotin-linked secondary Ab, strep-HRP and OPD as a substrate.  The
|| problem we are having is that the developing has slowed down incredibly.
|| Assays that once took 5 min to develop are now taking over 30 min and
|| still not coming all the way up.  We are in the process of testing all the
|| components but in a latest test two different batches of HRP-strep gave
|| the same results.  We are a bit stumped.....anyone have any ideas?
|| We would appreciate any ideas!
|| Thank you
|| -- 
|| ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|| Robin Blumenthal, Ph.D.                          
|| Department of Pediatrics, Rm S303
|| Stanford University 
|| 300 Pasteur Drive
|| Stanford, California 94305
|| robinb at leland.stanford.edu                 
|| (650) 723-8224    (650) 498-4632 FAX
|| ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


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