From fguerrero from biothera.com Wed May 6 12:25:02 2009 From: fguerrero from biothera.com (Faimola Guerrero) Date: Wed May 6 18:45:34 2009 Subject: [Immunology] Human Peripheral blood's Neutrophil isolation Message-ID: Hi I am trying to use your protocol to isolate neutrophils. What is the purpose of the ACD is it a substitute for heparin? -- Faimola Guerrero From sirgreat76 from hotmail.com Thu May 7 22:38:18 2009 From: sirgreat76 from hotmail.com (ali ali) Date: Fri May 8 08:55:44 2009 Subject: [Immunology] RE: Immuno Digest, Vol 45, Issue 1 In-Reply-To: <200905071704.n47H40b20802@net.bio.net> References: <200905071704.n47H40b20802@net.bio.net> Message-ID: Hi, There are many anticogulants e.g haparin, ACD and EDTA are few of them.Usage of ACD/EDTA/heparin actually depends what you want to do with isolated neutrophiles. In our experience of DNA typing, we are using ACD instead of EDTA. Acid Citrate Dextros has advantage especially in DNA amplification (PCR reactions) as EDTA cause amplification problem in amplification, and Heparin is expensive. with regards Raza Ali Scientific research officer SIUT. > Date: Thu, 7 May 2009 12:04:00 -0500 > From: immuno-request@oat.bio.indiana.edu > Subject: Immuno Digest, Vol 45, Issue 1 > To: immuno@magpie.bio.indiana.edu > > Send Immuno mailing list submissions to > immuno@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/immuno > or, via email, send a message with subject or body 'help' to > immuno-request@net.bio.net > > You can reach the person managing the list at > immuno-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Immuno digest..." > > > Today's Topics: > > 1. Human Peripheral blood's Neutrophil isolation (Faimola Guerrero) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 06 May 2009 12:25:02 -0500 > From: Faimola Guerrero > Subject: [Immunology] Human Peripheral blood's Neutrophil isolation > To: > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Hi I am trying to use your protocol to isolate neutrophils. What is the > purpose of the ACD is it a substitute for heparin? > > -- > Faimola Guerrero > > > > ------------------------------ > > _______________________________________________ > Immuno mailing list > Immuno@net.bio.net > http://www.bio.net/biomail/listinfo/immuno > > End of Immuno Digest, Vol 45, Issue 1 > ************************************* _________________________________________________________________ Hotmail? has a new way to see what's up with your friends. http://windowslive.com/Tutorial/Hotmail/WhatsNew?ocid=TXT_TAGLM_WL_HM_Tutorial_WhatsNew1_052009 From support from scipub.com Tue May 12 23:33:00 2009 From: support from scipub.com (support@scipub.com) Date: Wed May 13 09:26:07 2009 Subject: Memorandum of Understanding for Confrence "[Immunology] Acapulco and Hawaii 2009 Immunology Conferences" Message-ID: <20090513043300.51621.qmail@scipub.org> Dear Sir/Madam, We are pleased to inform you that "American Journal of Immunology" decided to dedicate a special issue for publication of all accepted articles presented at your conference for more citation and world wide readership. American Journal of Immunology is available in both, online and print format with full length articles for free and unlimited data download. American Journal of Immunology would bear printing and publication cost of all articles. Would you please kind enough to spare some time to respond and forward this e-mail, add our name in sponsors list and share your ideas with us. I look forward to hearing from you in very near future. Regards Editor Science Publications http://scipub.org From dobrien001 from rochester.rr.com Thu May 14 21:06:01 2009 From: dobrien001 from rochester.rr.com (David O'Brien) Date: Fri May 15 11:27:03 2009 Subject: [Immunology] CD56CD16 bright, CD56CD16 dim Message-ID: <20090515020601110.ZTOI8008@hrndva-omta01.mail.rr.com> We have a client who wishes to do a dot plot with CD56PE CD16PE on the y axis vs CD3 on x axis. The client wants to separate CD56CD16 bright cells from CD56CD16 dim cells. We have run some normal patients and found that only one population of cells shows up. There is no clear delineation between a bright and dim population for CD56CD16 staining. Has anyone done this before and does anyone know how to set the gate so that one could delineate bright and dim cells? From snehalrm from gmail.com Wed May 20 10:30:30 2009 From: snehalrm from gmail.com (snehal mhatre) Date: Wed May 20 10:54:03 2009 Subject: [Immunology] Non radioactive Cytotoxic activity assay Message-ID: <36383dd50905200830n6be6b5f3sc206828d81b020c6@mail.gmail.com> I am Snehal Mhatre, PhD student at IIH, Parel (Mumbai, India). I have to work on the activity of NK cells and I am looking out for a non-radioactivity method. Can you help me out me in this? Iwould like to know the different methods which I can use and also the reproducibility of the methods. thank you From linalit from yahoo.com Thu May 21 00:36:14 2009 From: linalit from yahoo.com (lit lei cheng) Date: Thu May 21 11:55:22 2009 Subject: [Immunology] Re: Immuno Digest, Vol 45, Issue 5 Message-ID: <221628.8621.qm@web32405.mail.mud.yahoo.com> Hi Snehal Mhatre, ? You can use lactate dehydrogenase assay (colorimetric assay) to detect the NKCA. ? regards, Lit ? --- On Thu, 21/5/09, immuno-request@oat.bio.indiana.edu wrote: From: immuno-request@oat.bio.indiana.edu Subject: Immuno Digest, Vol 45, Issue 5 To: immuno@magpie.bio.indiana.edu Date: Thursday, 21 May, 2009, 1:07 AM Send Immuno mailing list submissions to ??? immuno@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit ??? http://www.bio.net/biomail/listinfo/immuno or, via email, send a message with subject or body 'help' to ??? immuno-request@net.bio.net You can reach the person managing the list at ??? immuno-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Immuno digest..." Today's Topics: ???1. Non radioactive Cytotoxic activity assay (snehal mhatre) ---------------------------------------------------------------------- Message: 1 Date: Wed, 20 May 2009 21:00:30 +0530 From: snehal mhatre Subject: [Immunology] Non radioactive Cytotoxic activity assay To: immuno@magpie.bio.indiana.edu Message-ID: ??? <36383dd50905200830n6be6b5f3sc206828d81b020c6@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I am Snehal Mhatre, PhD student at IIH, Parel (Mumbai, India). I have to work on the activity of NK cells and I am looking out for a non-radioactivity method. Can you help me out me in this? Iwould like to know the different methods which I can use and also the reproducibility of the methods. thank you ------------------------------ _______________________________________________ Immuno mailing list Immuno@net.bio.net http://www.bio.net/biomail/listinfo/immuno End of Immuno Digest, Vol 45, Issue 5 ************************************* Get your new Email address! Grab the Email name you've always wanted before someone else does! http://mail.promotions.yahoo.com/newdomains/aa/ From bereza from enzyme-halle.mpg.de Fri May 29 04:32:09 2009 From: bereza from enzyme-halle.mpg.de (Magdalena Bereza) Date: Fri May 29 13:01:52 2009 Subject: [Immunology] fluorescent ELISA Message-ID: <01a57ce69690fb975e80931abf955765.squirrel@mail.enzyme-halle.mpg.de> Hallo, I would like to perform competitive ELISA with fluorescent labelled antibody fragment (VHH exactly). Have anybody done such assays? Could you provide me some tips which fluorescent dye should I use and which ELISA plate to use - I would prefer stereptavidin coated, but have no idea if they are suitable for fluorescent assays? Will be grateful for some hints. Magda