copy number of the gene of interest

limj03 at mcrcr6.med.nyu.edu limj03 at mcrcr6.med.nyu.edu
Thu Apr 9 14:49:50 EST 1998


Dear Maize Netters,

Now I am facing a puzzle, so that I would like to get any help from you. The
puzzle is as follows:

I have recently cloned and sequenced a maize gene with sequence homology to
the Arabidopsis gene SCARECROW. In order to study, its expression pattern,
I have tried to make a gene specific probe for in situ. To test whether it
is really gene specific, I performed Southern, but the probe (310bp)
recognized two bands. The probe is made based on the sequences of the 3'end
of the gene. It contains 3'UTR and coding sequences (half and half). The
simplest explanation is that there may be two very related genes in maize
genome. But I recently noticed that maize is more or less allotetraploidy.
Does it matter? Any comment is welcome.

Thank you!

Jun Lim
e-mail: limj03 at mcrcr6.med.nyu.edu
New York University
Bio Dept. Rm 1009
Washington Square East, NY10003






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