copy number of the gene of interest

Mike McMullen mcmullen at teosinte.agron.missouri.edu
Thu Apr 9 16:30:44 EST 1998


It only matters if you care which gene is actually expressed in a specific
tissue, developmental stage or condition.  Get rid of the coding region
and the 3' UTR will most probably be gene-specific.

Mike

On 9 Apr 1998 limj03 at mcrcr6.med.nyu.edu wrote:

> Dear Maize Netters,
>
> Now I am facing a puzzle, so that I would like to get any help from you. The
> puzzle is as follows:
>
> I have recently cloned and sequenced a maize gene with sequence homology to
> the Arabidopsis gene SCARECROW. In order to study, its expression pattern,
> I have tried to make a gene specific probe for in situ. To test whether it
> is really gene specific, I performed Southern, but the probe (310bp)
> recognized two bands. The probe is made based on the sequences of the 3'end
> of the gene. It contains 3'UTR and coding sequences (half and half). The
> simplest explanation is that there may be two very related genes in maize
> genome. But I recently noticed that maize is more or less allotetraploidy.
> Does it matter? Any comment is welcome.
>
> Thank you!
>
> Jun Lim
> e-mail: limj03 at mcrcr6.med.nyu.edu
> New York University
> Bio Dept. Rm 1009
> Washington Square East, NY10003






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