In my experience (specifically with maize the gene families for the
ubiquitins, shsps, and hsp 80s) a probe confined *strictly* to the 3'UTR
will generally be gene-specific at normal stringencies even when the ORFs
of the related genes have very high homology. I suggest you use pcr to
lift out the 3' UTR starting at a point just beyond the stop codon of your
coding sequence.
---
Robert A. Bouchard Phone: 330-263-2433 [Note: new]
Department of Biology FAX: 330-263-2378 [area code]
Mateer Hall eMail: RBOUCHARD at ACS.wooster.edu
931 College Street, The College of Wooster, Wooster, Ohio 44691-2363
