DNA from endosperm

Frances Bligh hfj.bligh at nottingham.ac.uk
Mon Apr 19 08:30:08 EST 1999


I've used the Nucleon Phytopure kit (available form Amersham Pharmacia
Biotech in the UK) on dried rice grain and got reasnable DNA from it.
Its fairly straightforward to use, especially if you have alot of
samples, although the DNAI got out was quite short, but as I mainly
use it for microsats this doesn't bother me. It's probably worth giving
it a go for maize. Try using a coffee grider to grind up the seed.
Frankie



On 12 Apr 1999 20:17:14 -0700, Amie Franklin
<franklin at candelab.berkeley.edu> wrote:

>Hi,
>I would like to genotype my mutator-gene knock out seeds directly by PCR
>analysis using DNA extracted from maize endosperm. Has anybody tried this
>or developed a protocol for extracting DNA from dry endosperm?
>
>Any help would be appreciated. thanks,
>Amie
>
>--
>Amie E. Franklin
>****************
>franklin at candelab.berkeley.edu
>345 LSA, MCB Dept.
>U.C.Berkeley
>Berkeley, CA 94720
>lab (510) 643-8277
>fax (510) 643-6791

Dr Frances Bligh,
Plant Science Division,
School of Biological Sciences,
University of Nottingham,
University Park,
Nottingham NG7 2RD
Tel: 0115 951 3216  e mail: hfj.bligh at nottingham.ac.uk




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