IUBio

Phenotype with AC or Mu gene knock outs

David Weber dfweber at ilstu.edu
Tue Feb 16 16:04:24 EST 1999


This obviously is a critical question, and I offer one small piece of
information that is somewhat relevant to this question.  Each of the
monosomic types have at least 50% aborted pollen; therefore, there is at
least one gene that is necessary for development of the gametophyte (that
does not have a functional duplicate locus on another chromosome) on each A
chromosome.  Also, hypoploids for B-A translocations (which have lost one
copy of a portion of one chromosome arm) also have at least 50% aborted
pollen, and B-A translocations for at least 19 of the 20 chromosome arms
have been recovered that behave in this way.  Thus, at least one gene that
is necessary for gametophyte deveopment (that does not have a functional
duplicate locus on an other arm) is present between the breakpoint and the
end of the chromosome arm for each of these.

>Dear All,
>             Given the predicated ancient tetraploid nature of the maize
>genome, I am interested in finding out how often an insertion into a gene (via
>Ac or Spm or Mu transposon tagging) leads to a detectable phenotype. Our very
>preliminary data suggests that we see a phenotype more often than we would
>predicate from the known number of single copy genes in the maize genome.
>Please note that our calculations already assume that Ac, Spm or Mu hits
>transcribed sequences 95% of the time.
>
>Any help would be appreciated
>
>Keith Edwards

David F. Weber, Professor of Genetics	Phone: 309-438-2685
Department of Biological Sciences	FAX: 309-438-3722
Campus Box 4120			email:  dfweber at ilstu.edu
Illinois State Univeristy
Normal, IL 61790-4120

Visit my web site:	http://www.bio.ilstu.edu/weber/




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