Postdoctoral Research Associate
Department of Plant and Microbial Biology
University of California, Berkeley, CA USA
A postdoctoral position is available immediately to participate in a
project focused on the generation and characterization of Ds-mediated
gene delivery in wheat. Recently it was shown that such a system
leads to a dramatic increase in gene expression stability in barley
(Koprek, T., et al., Transposon-mediated single-copy gene delivery
leads to increased transgene expression stability in barley. Plant
Physiology, 2001, accepted). In the present study, wheat plants
containing transgenes delivered with the Ds-system will be
characterized in terms of transgene expression stability relative to
barley and to genes introduced by Agrobacterium and microparticle
bombardment. Characterization of the physical nature of gene
insertion sites e.g., sequence, redundancy, methylation state and
chromatin condensation will be carried out, and comparisons of the
differences and similarities between the two plant species will be
done. Candidate will also be involved in studies focused on assessing
the impact of in vitro culturing and other stresses (e.g., nutrient,
water) on transgene expression stability of Ds-delivered transgenes
compared to those delivered by other methods. The successful
candidate will have monocot transformation experience and advanced
skills relating to the biochemical and molecular analysis of
transgenic plants and the creation and analysis of vectors. This
project is expected to last a minimum of two years with a possibility
of extension. Please send curriculum vitae (preferably by e-mail)
and the names, phone numbers and e-mail addresses of three references
to Dr. Peggy G. Lemaux, 111 Koshland Hall, University of California,
Berkeley, CA 94720; FAX: 510-642-7356; e-mail:
balonso at nature.berkeley.edu. The University of California is an
equal opportunity/affirmative action employer.
