To whomever it may concern,
Hello, I'm a graduate student at Texas Tech University, and I am having
trouble finding a protocol for callus induction of maize from
aseptically germinated seedlings. I use Relprim corn in my research and
my callus growth is very slow. I have used basal MS media with 30g/l
sucrose, 8g/l agar and 4mg/L 2,4-D and tried growing cultures in the
dark at 25C and 30C. I have also tried growing the cultures in a 16/8
photoperiod at 25C and 30C and still with no luck. My ultimate goal is
to study the binding of a radiolabeled substrate with suspension
cultured cells. I would greatly appreciate any advice.
Thank you,
Chris Truitt
Dept. of Chemistry
Texas Tech University
Lubbock TX 79416
806-742-9008
ironmanchris at hotmail.com
