June 8, 2007
Dear Maize Cooperators,
As many of you know, we have been developing a sequence-indexed
collection of Ds insertions (http://www.plantgdb.org/prj/
AcDsTagging/). Each line contains one or two novel Ds insertions
that are maintained in a uniform W22 inbred line. To date we have
generated sequence flanking approximately 900 Ds insertion sites. We
are actively developing resources to make screening this collection
user friendly. These resources include several periodically updated
web pages:
A BLAST page to query our fDs collection with your favorite genes or
BACs, etc. of interest:
http://www.plantgdb.org/ZmGDB-cgi/blastGDB.pl
Ds insertion sites mapped to BAC assemblies:
http://www.plantgdb.org/ZmGDB/DisplayAcDsAnn.php
Ds insertion sites mapped to GSS assemblies:
http://www.plantgdb.org/prj/AcDsTagging/tagsummary.php?
tagtype=DsFlankingSeq
We have also generated a browsable list of putative gene insertion
sites using the MAGI assemblies and their gene annotation as a
framework:
http://evlabg4-04.gdcb.iastate.edu/fDs_hits/
We invite and encourage the maize research community to explore the
collection. Seed stocks for all lines are currently available and
can be ordered from the Brutnell lab ($40/line academia; $100/line
industry). You will need to fill out an order form (http://
www.plantgdb.org/prj/AcDsTagging/order_instructions.php )and we will
distribute approximately 10 seed to you. All Ds insertions segregate
1:1 in our testcross populations, so you will need to self pollinate
all the plants to ensure recovery of the Ds insertion and to uncover
recessive mutations. When you order a line we will also propagate
the materials in either our summer or winter nursery to ensure that
the seed stocks are maintained and to provide you with additional
seedstocks (@100k) once we harvest the bulked materials. None of
this material is transgenic and no MTA is required.
Please keep in mind that this is a growing collection. Each month
for the next 2 + years we will be placing approximately 200 new Ds
insertions on the physical map, so check back often!
Your feedback is greatly appreciated.
Best regards,
Tom Brutnell, Boyce Thompson Institute, Cornell University
Erik Vollbrecht, Iowa State University
Volker Brendel, Iowa State University
