Cheung C. Yue
ccy at po.CWRU.Edu
Tue Feb 4 15:50:24 EST 1992
In a previous article, muriana at aclcb.purdue.edu (Peter M. Muriana) says:
> If I had a <mixed> DNA probe of <unknown> sequence, such as that
> which may be obtained by bacterial genomic subtraction, yet the
> amount of subtracted/mixed probe obtained was too little to obtain
> sufficient detection using chemiluminescence labelling.
> Could this mixed probe be amplified using a PCR adaptation similar
> to an oligo-labelling, except with Taq polymerase instead of
> Klenow and excess random primers? (even though the product may
> not identically represent the proportions of probe present in the
> original mixed probe).
I don't see why not, except I would probably ligate an adapter sequence
to the ends of the probe sequence (e.g. Science 246:780) and use that as
the PCR primer. Similar approaches have been applied to cDNA previously.
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