Glowing Blue DNA Gels
Eric R. Hugo
e_hugo at dsu1.dsu.nodak.edu
Thu Apr 21 05:18:39 EST 1994
I tend to feel that the glowing blue fluorescence that is sometimes seen
is more likely an artifact of contamination rather than too much EB.
Personally, I think it might be due to all of the fluorescent brighteners used
in everything from laundry detergent to toothpaste. I've noticed that if I
reposition a gel without a glove on (I know--Bad scientist <whap whap on the
wrist>;-) ) I often see blue glowing fingerprints on the edge of the gel. I
suspect that this is due to brightener contamination being transferred from my
clothes. Personally, I could do without all the additives but I guess I'm not
quite ready to start doing my laundry by beating my clothes on a rock.
More information about the Methods