Pharmarcia Red Sepharose CL-6B

[Ing-Nang Wang]

I wonder if anyone has experience on Pharmacia Red Sepharose CL-6B? 
Recently, I tried to partially purify 6-phosphogluconate dehydrogenase (use
NADP) from E. coli.  However, I found it very difficult to elute the enzyme
from the column.  The elution buffer I used is HEPES 50 mM + MgCl2 10 mM +
NADP from 1 to 3 mM.  Any idea on this?

-- 
Ing-Nang Wang
ingnang at sbbiovm.sunysb.edu