T-Vector for PCR cloning

[Seth Findley]

I have seen a few postings recently regarding T-vectors.

I have one almost made, but have little interest in finishing the 
construction. If somebody wants to finish this, I can send the stuff:

I cloned two T end-producing restriction sites (Eam1105I) into the polylinker
of pBS.(There were very few choices for how to do this).* 

There is unfortunately still another Eam1105I site in pBS ampicillin(R) gene 
that still needs to be converted. 

I have made an oligo to delete the site via a silent substitution (by any 
number of techniques).  

If somebody wants to do the mutagenesis, I can send you: the oligo, the 
T-site polylinker and a cheery "good luck to you!"

* I used an couple of oligos to introduce a small fragment into pBS-II-SK 
polylinker. The fragment (20 bp) contains two Eam 1105I sites (NEB) in an 
inverted orientation relative to one another.(look up the site).
Because the recognition site has a few internal N's I made them produce 
ends which have the required T overhangs. 


-Seth