alternative to Whatman 3MM paper for drying down gels?
tan at aeolus.vmsmail.ethz.ch
Wed Feb 9 03:03:47 EST 1994
In article <Rasmussen-080294114008 at ls-10.biology.utah.edu>
Rasmussen at Bioscience.utah.edu (Randy P. Rasmussen) writes:
>In article <2j5ivn$jit at elna.ethz.ch>, tan at aeolus.vmsmail.ethz.ch (Song Tan)
>> Does anyone have any suggestions for cheaper alternatives to Whatman
>> 3MMChr (commonly known as 3MM) paper for drying sequencing and bandshift gels?
>Why don't you try gluing your gels to one of the glass plates (Garoff and
>Ansorge, Analytical Biochemistry 115, 450-457, 1981).
[details of gluing gels to plate deleted]
Unfortunately, we use 50x20 cm sequencing glass plates and our X-ray cassettes
are only 43 cm long. We slice off the top 10 cm of the gel since there's never
any readable sequence at the top anyway. Gluing the gels to the plates would
pose a problem when it's time to expose the gels in the cassettes (glass plates
won't fit into the cassettes. we could use other containers to expose the
gels, but we like using the cassettes). Nevertheless, many thanks for your
response, Randy. I'm sure it will come in useful in the future.
What I was really hoping for was some other source or kind of filter paper as a
substitute for 3MM. It seems a little silly to me that we're using "high"
quality chromatography paper to dry down gels. True, it works and perhaps we
shouldn't mess with a good thing. Frances Hannan's posting of using the paper
between X-ray films does sound promising, but I would still be interested in
hearing from anyone with other ideas.
Institute for Molecular Biology and Biophysics
ETH-Honggerberg (Swiss Federal Institute of Technology)
8093 Zurich, Switzerland
email: tan at aeolus.vmsmail.ethz.ch
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