McIlvaine buffer

[Ross Whetten]

The CRC Handbook of Biochemistry gives the composition of McIlvaine buffer
as a mixture of 0.1 molar citric acid and 0.2 molar disodium phosphate.
Depending on the pH you want, you mix varying amounts of the two components
together. For example, 98 ml of citric acid + 2 ml of Na2HPO4 gives pH 2.2,
42 ml of citric acid + 58 ml Na2HPO4 gives pH 5.6, and 2.75 ml citric acid
and 97.25 ml Na2HPO4 gives pH 8.0 (all pH determinations given at 21 C).



Ross Whetten 
Research Assistant Professor 
Forest Biotechnology Group
North Carolina State University
Raleigh, North Carolina 27695-8008  USA
telephone or fax (919)515-7801
e-mail rosswhet at unity.ncsu.edu
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Ross Whetten 
Research Assistant Professor