PEG : to autoclave or not to autoclave, that is the ques

[Jarmo Niemi]

In <2jou5u$lpk at mule.fhcrc.org> Tim writes:

> Is there a reason why protocols for PEG precipitation of phage specify
> that it should be filter sterilized? 
> What's supposed to happen if you autoclave it?????

In my experience, solutions with PEG and NaCl, even when autoclaved
separately, turn yellow after some time. Filtered PEG-NaCl solutions
seem to do this much more slowly. What happens, I don't know, and DNA
prepared with yellow PEG-NaCl can be sequenced, IMH (In my hands...).

Jarmo Niemi   Biochemistry, University of Turku, Finland, janiemi at finabo.abo.fi