Help: mutations in pATH vectors?

Duncan Clark Duncan at genesys.demon.co.uk
Wed Feb 16 14:13:47 EST 1994


Hi Loren,

LB has tryptophan in sufficient amounts to induce your expression. Try growing
in a minimal media plus casamino acids. Casamino acids are tryptophan free.
That should then allow induction with tryptophan or even tryptone.

Duncan 
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Duncan Clark                        | Internet:    duncan at genesys.demon.co.uk
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