PEG : to autoclave or not to autoclave, that is the question...

Bernard Heymann bheymann at bragg.bio.purdue.edu
Wed Feb 16 11:31:15 EST 1994


In article <2jou5u$lpk at mule.fhcrc.org>, Tim Buss <tbuss at fred.fhcrc.org>
wrote:

> Is there a reason why protocols for PEG precipitation of phage specify
> that it should be filter sterilized? 
> What's supposed to happen if you autoclave it?????

PEG and related detergents form peroxides and aldehydes very easily when
treated harshly.

-- 
Bernard Heymann
bheymann at bragg.bio.purdue.edu



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