How to Avoid PCR Errors

[Brian Foley]

	In reporting 12 mutations in 23 kb of sequenced PCR product
I must also state that my data are skewed because my method was not
aimed at publishing a TAQ error rate:
	I stopped sequencing many clones as soon as I encountered
a PCR mutation.  Thus my results are biased toward sequencing clones
without mutations and not seuqncing clones that may have had more
than one mutation.
	I had Reverse Transcribed poly-A mRNA and the PCR amplified 
a 2.6 kb cDNA.  I then cloned the PCR products and sequenced them. 
These mutations could have been introduced either by the RT (MoMULV) 
the TAQ polymerase, or by the E.coli.

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*  Brian Foley               *     If we knew what we were doing   *
*  Molecular Genetics Dept.  *     it wouldn't be called research  *
*  University of Vermont     *                                     *
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