autoclaving and PEG

John Brunstein brunstei at UNIXG.UBC.CA
Wed Feb 23 14:02:45 EST 1994


	OK, I've heard first-hand of fungal spores that could survive
autoclaving (in fact, the problem was only apparent if the media was
autoclaved and then stored at 60C for a few hours before being poured,
suggesting that the bugs needed the heat to induce growth) and while 5%
SDS sounds pretty awful I can believe there exist a few bugs which could
live in it.....BUT concentrated KOH?  Where's the carbon source.....CO2?
If you could actually isolate a strain able to live under those conditions
it ought to be worth a Nature paper.
	Just my Solution III induced thoughts on the subject...






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