Quantitating small amounts of DNA
basil at ovisun.ovi.ac.za
Mon Feb 28 08:56:43 EST 1994
We take up small amounts of purified DNA in <100ul and measure A260/A280 using
a micro-cell which takes a minimum of about 60ul with a path length of 1cm.
This enables us to use all the DNA we have recovered.
If your spekker is accurate down to an absorbance of 0.005 this will convert
to about 12ng of DNA in 60ul. If you have less than this you don't have
enough for anything much!
More information about the Methods