LMP agarose gels run in TAE buffer (used to be:Purification from Low Melt)
smrodems at students.wisc.edu
Mon Feb 28 23:20:13 EST 1994
In article <vernon.16.0009A61A at micro.uct.ac.za>, vernon at micro.uct.ac.za
(Vernon Coyne) wrote:
> This is something that I have always done, but not known the reason why it is
> so. Could you please enlighten me as to the reason one should prepare LMP
> agarose gels in TAE instead of TBE when purifying DNA fragments from the gel.
> (I also always use TAE gels for Southern blotting to nylon membranes).
I have heard that borate can cause DNA damage so that some people stay away
from borate containing buffers when the want to use the DNA for further
cloning. However, I have been using TBE recently with the GeneClean II
system and have had no problems. For me its easier to use TBE because our
lab makes it up in 20L carboys. Also, I believe TBE gives better
resolution so I've stopped using TAE altogether.
Steve "Some day I will get the hell out of Wisconsin" Rodems
"Then I am here for the Lee family renioun, shur-wajo-shur"
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