Sequencing problem, HELP!!

[Zainul Zain]

Greeting to all bio netters,

I recently had a problem with my sequencing gel. When I load my sample 
they did not run in a straight line as they should be, instead, they 
dispersed from the straight line.

                     
                     _     
                 _       _
               _            _   This is how they looks like on running gel!

This happen to me when I change the acralymide soln. to the new long 
ranger solution. I prepare 6% gel and followed exactly the protocol 
provided by the manufacturer.

Please advice!!!

You can e-mail to the addres above.
Many thanks!!


Robaiza 
Medical School
University Sains Malaysia
Kelantan, Malaysia