Taq left at RT - gulp! -

[Susan Cole]

In article <9409280157.AA27179 at cscgpo.anu.edu.au>,
 <Klaus.Matthaei at ANU.EDU.AU> wrote:
>>Geez what a boo-boo...
>>Accidentally left Taq out (covered though) at 25C for 24hr!!!
>>Primers, buffersw, etc as well...


Considering the Taq normally goes up to 94oC thirty times during a PCR it 
should be fine (and probably is -- I've used Taq that was left at RT for 
several days without any problems).  The nucleotides, however, go bad 
very rapidly at RT, and I would probably pitch them.  

Susan