cDNA Challenge*************

[Andy Law Big Nose]

In article <3623o2$l5t at dns1.NMSU.Edu> Shahram Mori, smori at nmsu.edu writes:
>
>Dear Molbionetters,
>I have been using the Perkin Elmer RNA PCR kit to make cDNA from my Total
>RNA samples. My RNA is in good condition and has been DNase treated. I
>follow the protocol in GeneAMP kit and yet achieve no cDNA's.

How did you kill the DNAse? We have had some big problems with DNAse
spilling over after it had supposedly been killed and digesting all the
cDNA products.

Andy Law

( Lawa @ bbsrc.ac.uk                     Big Nose in Edinburgh )