Extraction of DNA from agarose gels

[futers at biovax.leeds.ac.uk]

In article <36em01$5p at emory.mathcs.emory.edu>, medtjm at bimcore.emory.edu (T. J. Murphy) writes:
>In article 15518 at leeds.ac.uk, futers at biovax.leeds.ac.uk writes:
>> In article <GABRE.40.2E87E76D at wwg3.uovs.ac.za>, GABRE at wwg3.uovs.ac.za writes:
>> >Hi,
>> >
>> >We are currently looking for an efficient and fast method for the isolation 
>> >of DNA fragments from agarose gels.> >
>> 
>> The quickest method I know is as follows:-
>>     1. Cut band out of agarose gel.
>>     2. Put into 1.5ml tupe and freeze in liquid nitrogen.
>>     3. Place frozen agarose slice onto a piece of Nescofilm.
>>     4. Squeeze between finger and thumb and as the liquid is
>>        squeezed out, collect with a pipettor.
>> 
>>   You are left with only a small amount of agarose in the Nescofilm
>> and you should get a high recovery of the DNA which can be used 
>> directly in ligations etc.
>>            Hope this helps,
>>                   Simon Futers (futers at biovax.leeds.ac.uk)
>> 
>> 
>
>What is the best agarose to use for these kinds of crush protocols????
>
>
>TJ Murphy
>Asst. Professor	      
>Dept of Pharmacology
>Emory University School of Medicine   	    
>
>

I have always used "ordinary" agarose.  I do not think there is any best
agarose for this technique.
                     Simon Futers (futers at biovax.leeds.ac.uk)