extraction of DNA from agarose gels

[g_watson at icrf.icnet.uk]

We use DEAE paper.  Cut the gel just below the band of interest.  Then insert a
piece of DEAE paper (Schleicher  & Schuell NA 45 Ref 417 082).  Run the gel a
little longer until the band is in the paper (you can see the ethidium bromide
in the paper in daylight sometimes). (Yes we put the ethidium bromide in the
gel).  
	Then just cut the paper up and put it into an Eppendorf tube and heat
at 70 deg C for 15 mins in 250 microL of 0.5M NaCl.  I do this twice and then
precipitate with 500 microL of ethanol.  Yield is fine, and I use this for
ligations all the time.  In fact I even clean up the opened vector like this
since its no more hassel to do it with the vector and the insert.
	Graham Watson
	ICRF
	London
	G_Watson at ICRF.ICNET.UK