small DNA fragment recovery

[lesley brown]

In article <ds28-300994103719 at 132.236.156.63>, ds28 at cornell.edu (David B.
Stern) wrote:

> What is the best way to get high yields of small DNA fragments (50-100bp)
> isolated from agarose gels?
> I have tried Qiagen and other glass products, but my yields always seem way
> low.
> Do freeze squeeze, phenol extractions or Gelase type products work as well?
> Thanks
> phk1 at cornell.edu
> Phil Kogan

I haven't tried the low melting point protocol listed in the reply, but I
have used beta agarase (to digest the gel slice) which usually works well. 
Alternatively, I use NA45 paper from Schleicher and Schuell to "catch" the
DNA (stick it in the gel below the band and electrophorese for a few
minutes) and elute the DNA in high salt buffer.  The recovery is probably
not as good as beta agarasing the gel slice, but it's less messy.  Let me
know if you want a more detailed protocol.

Suzy