cDNA Challenge*************

[Shahram Mori]

Andy Law (Big Nose) (lawa at bbsrc.ac.uk) wrote:
: In article <3623o2$l5t at dns1.NMSU.Edu> Shahram Mori, smori at nmsu.edu writes:
: >
: >Dear Molbionetters,
: >I have been using the Perkin Elmer RNA PCR kit to make cDNA from my Total
: >RNA samples. My RNA is in good condition and has been DNase treated. I
: >follow the protocol in GeneAMP kit and yet achieve no cDNA's.

: How did you kill the DNAse? We have had some big problems with DNAse
: spilling over after it had supposedly been killed and digesting all the
: cDNA products.

: Andy Law

: ( Lawa @ bbsrc.ac.uk                     Big Nose in Edinburgh )


Dear Andy, 
I have been ETOH and NaAcetate pptating my RNA after I did two extractions
of Phenol/Chloroform to get rid of the proteins.I hope that it did it.
cheers,
--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003