RT-PCR question

[Sean David Moore]

If all of the reagents are "ok", then the reaction will work..unless the 
target isnt there.   



Adi Santoso (santoso at badlands.NoDak.edu) wrote:
: i try several time RT-PCR with 3' primer using oligo dT (16 mers) just 
: never work. I check all the reagents and template RNA, they are all 
: oke...!!! any suggestion for me.  I just almost give up with it..

: ADI
: Dept. of Biochemistry
: North Dakota State University

--
Sean Moore

VAMC Philadelphia,
Medical College of Pennsylvania,
and the University of Pennsylvania...

.

smoore at sas.upenn.edu