Sequencing problem, HELP!!

[gli at UCDAVIS.EDU]

On 1 Oct 1994, Zainul Zain wrote:

> Greeting to all bio netters,
> 
> I recently had a problem with my sequencing gel. When I load my sample 
> they did not run in a straight line as they should be, instead, they 
> dispersed from the straight line.
> 
>                      
>                      _     
>                  _       _
>                _            _   This is how they looks like on running gel!
> 
> This happen to me when I change the acralymide soln. to the new long 
> ranger solution. I prepare 6% gel and followed exactly the protocol 
> provided by the manufacturer.
> 
> Please advice!!!
> 
> You can e-mail to the addres above.
> Many thanks!! 

Your glass plate is not good enough. It happened here before. After we 
change a new glass plate. Everything is OK.

Gang Li
Bodega Marine Lab. 
UC Davis