ABI sequencing and Stratagene Taq Extender

[Dirk Bornemeier]

In article <405625695wnr at genesys.demon.co.uk>, Duncan at genesys.demon.co.uk wrote:

> In article: <anderson-290994104324 at 128.252.181.14> 
anderson at pharmdec.wustl.edu (Eric C. Anderson) writes:
> > distance.  from what i understand of both products, there isn't anything
> > obvious that would make one or the other of them screw things up, and it
> > seem that if it worked (i.e. gave another 100bp or so of good sequence) it
> > would be a cheap and easy way to stretch the sequence, as opposed to
> > switching to the Dye Primer or Sequenase chemistry kits.
> 
> There was a thread similar to this a few weeks back but results never 
> appeared. Basically taq extender is dilute Pfu approx 1/16. Pfu has a 
> 3-5' exonuclease and as such will remove mis-incorportaed bases 
> including ddNTP's. This used to be a standard test for whether your 
> unknown pol had proof-reading exo or not.


I have been told that ABI is reformulating their Taq kits using a Taq that
has proofreading capabilities and these kits will be rolled out soon. 
Details are currently unavailable.


Dirk Bornemeier
Ann Arbor, MI