Glowing BLUE DNA Gels

[Klaus Salger]

Subject: Re: Glowing BLUE DNA Gels
Newsgroups: bionet.molbio.methds-reagnts
References: <Cx3wov.yo at ncifcrf.gov>
Distribution: world

I would like to know if everybody who got these blue glowing gels used the
same TAE-recipe as John
:  					50X
:  
:  	Trizma HCL			228.8 g
:  	Trizma base			66.4 g
:  	Na acetate (anhydrous)		82.0 g
:  	0.5 M EDTA, pH 8.0		200 ml
:  	dH2O				to 1 litre.
I prepare may 50xTAE as described in Maniatis, Tris base, acetate, EDTA
(no Na+, no Cl-) and didn't ever see this blue glow though sometimes using
rather old 1xTAE, running it for several times, sometimes running it very
hot and preparing the gels by boiling in the microwave.
May be this slight difference in buffer composition leads to problems under
certain conditions.
Cheers
  Klaus

--
Klaus Salger
Zoologisches Institut
AG MacWilliams
Luisenstr. 14
80333 Muenchen
Germany

phone : ++49 (0)89 5902 -502
FAX   :                 -450
e-mail: salger at zi.biologie.uni-muenchen.de