Shahram Mori (smori at opus.nmsu.edu) wrote: : I agree completely with Annette. Use 6mers for cDNA synthesis ( 1hr at : 25C) and forget oligo dt's. Make your library using random 10mers. : cheers, I meant 35C not 25. Sorry! cheers, -- Shahram Mori _/\_ Program in Molecular Biology _\ /_ Dept. of chemistry and Biochemistry Box 3C \_ _/ NMSU Las Cruces NM || 88003