ANCHORED-PCR

[Shahram Mori]

sl6dp at cc.usu.edu wrote:
: I am planning to use 'anchored PCR' to isolate isoforms of a gene,
: the standard primers that I have found in the literature (Loh et al.)
: seem to form a loop, besides, they have very high Tm (Oligo program). 
: Have anyone used these primers, do they really work?
Why don't you design your own anchors? you can use Oligo design programs
to design them and get rid of these nasty 2ndary structures.
cheers,


--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003