How annoying (was Re: RT-PCR question)

[Michael Poidinger]

In article <36s199$647 at netnews.upenn.edu> smoore at mail1.sas.upenn.edu (Sean David Moore) writes:
>From: smoore at mail1.sas.upenn.edu (Sean David Moore)
>Subject: Re: RT-PCR question
>Date: 4 Oct 1994 16:52:57 GMT

>If all of the reagents are "ok", then the reaction will work..unless the 
>target isnt there.   

Well thank you very much David. How insightful. Well worth the bandwidth to 
post such helpful advice as this.  See everyone? you are doing it wrong! 
rather than trying to help people with their problems, just tell them that it 
should work OK if their reagents are OK, and everything will be OK!

flame off..

In reply to Adi, it would help us if you posted more info about your 
technique, what kit you are using if any, PCR conditions, reagent 
concentrations etc etc.


>Adi Santoso (santoso at badlands.NoDak.edu) wrote:
>: i try several time RT-PCR with 3' primer using oligo dT (16 mers) just 
>: never work. I check all the reagents and template RNA, they are all 
>: oke...!!! any suggestion for me.  I just almost give up with it..

Mike


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Dr Mike Poidinger        
Microbiology, UWA       ==> Animal Welfare NOT Animal Rights       
Australia                        
mikep at uniwa.uwa.edu.au 
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