DNA purification

[Wiggy]

In article <devitta-1210941350360001 at bcs93.bham.ac.uk>,
devitta at uk.ac.birmingham (Chutney) wrote:

> I need help.
> Can anyone give me a simple and fool proof method for the isolation
> of large DNA.  The piece I am bothered about is just under 30 kb
> and at present I am using a low % agarose gel and then prep-a-gene but I
> seem to be getting low yields.
> Any help would be appreciated.
> Please mail me a reply
> Thanks

What do you want to use the recovered DNA for? This may influence the
technique that you use to get it back from the gel, but if you want the
most fool proof method with good yield have you tried the freeze squeeze
method that was posted to this group a week or so ago.

Wigs (Upstairs!)