ANCIENT DNA

[Tracy Aquilla]

In Article <cmeghen-171094154018 at gen023.gen.tcd.ie>, cmeghen at mail.tcd.ie
(Ciaran Meghen) wrote:
>QUESTION? What do YOU think of using autoclaving as a technique to wholly
>or partially remove contaminating nucleic acids from solutions. Does it
>work? I do it anyway just because I'm paranoid.
>
>************************************************************************
>Chris Troy,           E-mail: catroy at mail.tcd.ie
>Genetics Department,     Phone:  (353)-1-7021265
>Trinity College,         Fax:    (353)-1-6798558
>Dublin 2.
>Ireland.
>*************************************************************************

Autoclaving will have little effect on DNA in solutions. I do boiling
mini-preps of plasmid DNA quite frequently, and the yields are usually very
high.
    Tracy