ammonium sulfate precipitation of proteins

[Flip Hoedemaeker]

In article <Cxzs46.HHr at phibred.com>, bipin dalmia <dalmiabk at phibred.com> writes:
>[...]
>centrifugation at 20,000 g for 20 minutes and NO PELLET!!! can someone
>explain what happened and how i can recover my protein?
>bipin

I think keeping your mixture on ice for at least an hour will improve things.
Maybe you should also increase your G-force!

Flip