Free sulfhydryl determination

[Shaun D. Black]

We find that Ellman's reagent (dithio-bis-nitrobenzoate; DTNB) works well
for this application.  Certain problems have been noted, especially at
extremes of pH, but in our hands, the method is fast and quantitative.  Let
me know if you'd like any methodologic details.  Best regards,  Shaun

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  = Shaun D. Black, PhD   | Internet address:     shaun at jason.uthct.edu = 
  = Dept. of Biochemistry | University of Texas Health Center, at Tyler = 
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