DNA/phenol problem

[Chuck Cerveny]

> In article <38ftvv$c2 at er1.rutgers.edu>, jwelsh at er1.rutgers.edu (Jennifer_ Welsh) writes:
> > Dear Netters,
> > 
> > I've been doing a standard (mod. Birnboim and Doly) alkaline lysis DNA
> > prep on 40ml E. coli cultures.  All of a sudden I was having trouble
> > getting a decent yield.  I did the protocol in parallel using my
> > solutions and another person's.  An agarose gel shows the yield to be
> > the same before phenol extraction but not after (the same phenol was
> > used for both preps).  Does anyone have any ideas about what is going
> > wrong?  I checked the pH of the NaOAc.  It's correct.  All advice
> > gratefully accepted.
> > 
> > Thanks in advance,
> > 
> > Jennifer Welsh
> > 
> > -- 
> > If you want me, look at welsh at biovax.rutgers.edu
> 
Sounds like you may have a problem with your phenol pH.  See if you can
either borrow someone else's phenol for a run or equilibrate a new bottle
of the stuff.
-- 
Chuck Cerveny
ccerveny at u.washington.edu

"Good times and riches and son of a bitches, I've seen more than I can
recall"  J. Buffett